Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   JYK05_RS11170 Genome accession   NZ_CP071129
Coordinates   2404288..2405487 (+) Length   399 a.a.
NCBI ID   WP_206467061.1    Uniprot ID   -
Organism   Caballeronia sp. M1242     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2399288..2410487
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JYK05_RS11140 (JYK05_11140) cgtA 2399513..2400619 (-) 1107 WP_206467058.1 Obg family GTPase CgtA -
  JYK05_RS11145 (JYK05_11145) rpmA 2400821..2401084 (-) 264 WP_175945147.1 50S ribosomal protein L27 -
  JYK05_RS11150 (JYK05_11150) rplU 2401116..2401427 (-) 312 WP_008342953.1 50S ribosomal protein L21 -
  JYK05_RS11155 (JYK05_11155) - 2401663..2402655 (+) 993 WP_206467059.1 polyprenyl synthetase family protein -
  JYK05_RS11165 (JYK05_11165) - 2402916..2404202 (+) 1287 WP_206467060.1 HlyC/CorC family transporter -
  JYK05_RS11170 (JYK05_11170) pilF 2404288..2405487 (+) 1200 WP_206467061.1 GspE/PulE family protein Machinery gene
  JYK05_RS11175 (JYK05_11175) - 2405487..2406698 (+) 1212 WP_206467062.1 type II secretion system F family protein -
  JYK05_RS11180 (JYK05_11180) - 2406730..2407641 (+) 912 WP_206467063.1 A24 family peptidase -
  JYK05_RS11185 (JYK05_11185) coaE 2407658..2408272 (+) 615 WP_206467064.1 dephospho-CoA kinase -
  JYK05_RS11190 (JYK05_11190) zapD 2408436..2409191 (+) 756 WP_175945315.1 cell division protein ZapD -
  JYK05_RS11195 (JYK05_11195) - 2409261..2409452 (+) 192 WP_175945160.1 DNA gyrase inhibitor YacG -
  JYK05_RS11200 (JYK05_11200) - 2409459..2409908 (-) 450 WP_206467065.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 399 a.a.        Molecular weight: 42795.14 Da        Isoelectric Point: 7.4886

>NTDB_id=542967 JYK05_RS11170 WP_206467061.1 2404288..2405487(+) (pilF) [Caballeronia sp. M1242]
MNSAPPAAFPRPADPTFETTSDGHLPAVSLLADTLREAAERGASDIHVEPSEHEWRIRLRVDGALHVLRRPPPHLRDAFV
TRIKVLARMDIAERRVPQDGRLRLALPGGQTGDYRVNSLPTLFGEKLVLRRLDALPRDLSLGALGLSDEQSSVVESAIRA
PHGLVLVTGPTGSGKTLSLYCFLQMLNAEARNICAVEDPAEIQLAGINQVSVREKAGLTFAVALRAFMRQDPDVIMVGEI
RDAETADVAVKAAQTGHLVLSTLHTNDAPAAIARLIDIGVAPYNLASALRLVTAQRLVRKLCDTCRAPSALTLPDGFRPF
EPRGCPACHGIGYRGRVGVHQLMPVSDGIRELIVGRAATHAVARLAQSEGMPALRDAAFARVREGVTSVAEAADATEIA

Nucleotide


Download         Length: 1200 bp        

>NTDB_id=542967 JYK05_RS11170 WP_206467061.1 2404288..2405487(+) (pilF) [Caballeronia sp. M1242]
ATGAATTCCGCTCCTCCCGCCGCGTTCCCACGCCCGGCCGACCCCACCTTCGAGACAACGTCCGACGGGCACTTGCCCGC
CGTTTCGCTGCTCGCCGACACCTTGCGCGAAGCCGCCGAGCGCGGCGCGTCGGATATTCACGTCGAGCCGTCCGAGCACG
AATGGCGCATCCGGCTGCGCGTGGATGGCGCGCTGCATGTGCTGCGCCGTCCGCCGCCGCATTTGCGCGATGCCTTCGTC
ACGCGAATCAAGGTGCTCGCGCGCATGGATATCGCAGAGCGGCGCGTGCCGCAGGACGGACGTCTGCGGCTCGCCTTGCC
GGGCGGCCAGACCGGCGACTATCGCGTGAATTCACTGCCGACGCTCTTCGGCGAGAAGCTGGTTCTCCGCCGGCTCGATG
CGTTGCCGCGAGACCTGTCGCTCGGCGCGCTCGGGCTCAGTGACGAGCAAAGCAGCGTTGTCGAATCCGCCATCCGCGCG
CCGCACGGCCTCGTGCTCGTGACCGGGCCGACCGGCAGCGGCAAGACGCTGTCGCTGTACTGTTTCCTTCAGATGCTCAA
CGCCGAAGCGCGCAACATCTGCGCCGTGGAAGATCCCGCCGAGATCCAGCTTGCGGGCATCAATCAGGTCAGCGTGCGCG
AAAAGGCCGGCCTGACCTTCGCCGTCGCGCTGCGTGCGTTCATGCGGCAGGACCCGGACGTCATCATGGTCGGCGAGATC
CGCGACGCCGAAACCGCCGACGTCGCCGTGAAAGCCGCGCAGACGGGCCACCTCGTTCTCTCGACGCTCCACACCAACGA
CGCGCCCGCCGCCATCGCGCGCCTGATCGACATCGGCGTCGCGCCGTACAACCTCGCGTCGGCGTTGCGGCTCGTGACGG
CGCAACGGCTCGTGCGCAAGCTCTGCGACACGTGCCGCGCGCCGTCCGCGCTGACACTCCCTGATGGCTTTCGTCCGTTC
GAGCCGCGCGGCTGCCCGGCGTGTCACGGCATCGGATATCGCGGGCGCGTCGGCGTGCATCAGTTGATGCCGGTGTCGGA
CGGCATTCGCGAGTTGATCGTCGGCCGCGCGGCGACTCACGCGGTTGCGCGGCTCGCGCAATCCGAAGGCATGCCCGCCC
TCCGCGACGCCGCTTTCGCGCGCGTGCGCGAAGGCGTGACGAGCGTCGCGGAAGCCGCCGACGCGACGGAGATCGCGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-300)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

48.861

98.997

0.484
  pilB Vibrio cholerae strain A1552

49.741

96.742

0.481
  pilB Acinetobacter baumannii D1279779

48.187

96.742

0.466
  pilB Acinetobacter baylyi ADP1

48.312

96.491

0.466
  pilB Vibrio parahaemolyticus RIMD 2210633

47.273

96.491

0.456
  pilB Vibrio campbellii strain DS40M4

47.273

96.491

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

43.796

100

0.451
  pilB Legionella pneumophila strain ERS1305867

45.833

96.241

0.441
  pilB Haemophilus influenzae Rd KW20

40.506

98.997

0.401
  pilB Haemophilus influenzae 86-028NP

40

98.997

0.396
  pilF Thermus thermophilus HB27

41.645

94.486

0.393
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.645

94.486

0.393
  pilB Glaesserella parasuis strain SC1401

41.689

91.98

0.383