Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JYB86_RS01735 Genome accession   NZ_CP070997
Coordinates   366591..368297 (-) Length   568 a.a.
NCBI ID   WP_005181778.1    Uniprot ID   -
Organism   Acinetobacter indicus strain LYS68A     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 361591..373297
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JYB86_RS01705 (JYB86_01705) - 361739..362062 (+) 324 WP_005181767.1 pyrimidine/purine nucleoside phosphorylase -
  JYB86_RS01710 (JYB86_01710) rlmB 362168..362920 (+) 753 WP_205779322.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  JYB86_RS01715 (JYB86_01715) - 362938..363858 (+) 921 WP_016659657.1 DMT family transporter -
  JYB86_RS01720 (JYB86_01720) coaE 363855..364463 (-) 609 WP_075175011.1 dephospho-CoA kinase -
  JYB86_RS01725 (JYB86_01725) pilD 364474..365334 (-) 861 WP_005181774.1 prepilin peptidase Machinery gene
  JYB86_RS01730 (JYB86_01730) pilC 365334..366560 (-) 1227 WP_127801106.1 type II secretion system F family protein Machinery gene
  JYB86_RS01735 (JYB86_01735) pilB 366591..368297 (-) 1707 WP_005181778.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JYB86_RS01740 (JYB86_01740) tpiA 368587..369378 (+) 792 WP_005181780.1 triose-phosphate isomerase -
  JYB86_RS01745 (JYB86_01745) secG 369392..369721 (+) 330 WP_005181783.1 preprotein translocase subunit SecG -
  JYB86_RS01770 (JYB86_01770) rimP 370590..371114 (+) 525 WP_205779323.1 ribosome maturation factor RimP -
  JYB86_RS01775 (JYB86_01775) nusA 371147..372631 (+) 1485 WP_005181786.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63758.21 Da        Isoelectric Point: 4.9207

>NTDB_id=542077 JYB86_RS01735 WP_005181778.1 366591..368297(-) (pilB) [Acinetobacter indicus strain LYS68A]
MSVQQAMPRFTGFIRRLVEEGLVTAENMQQAIVAAKKAETDIVPYLIQQLQISPLTIAEKISYEFGEPIFDLAVYDSRLI
VREGLDEKLFTKYRILPIFRRHNQLYIATSNPTNIEAIDAIRFNSKLNIEVVIVEHDKLERLIEQNFTEDSTFDFDEDFD
LEVGDNAPETQKDDDEPQGDEAPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRIDGVLRQIANPPLQLATRLASRL
KVMSQMDISEKRIPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKELFMEALDKPQG
MLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCQKCKAPVEIPRQSLLELGFTE
EDLNQPEFQLYQPVGCPECREGYKGRVGVYEVMKVTPEISRIIMEDGNALQIAAASAKSGFNNLRRSGLIKVMQGVTSLQ
EVNRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=542077 JYB86_RS01735 WP_005181778.1 366591..368297(-) (pilB) [Acinetobacter indicus strain LYS68A]
ATGTCAGTACAACAAGCAATGCCTAGATTTACAGGTTTTATTCGGCGACTGGTCGAAGAAGGGCTTGTGACGGCTGAAAA
TATGCAGCAAGCCATTGTCGCGGCGAAAAAAGCCGAGACCGATATTGTGCCGTATTTAATTCAGCAGTTGCAGATTTCAC
CGCTGACCATTGCTGAAAAAATTTCCTATGAATTTGGTGAACCGATTTTTGACTTGGCGGTGTACGATTCGCGTCTGATT
GTACGTGAAGGTCTGGATGAAAAACTGTTCACCAAATACCGAATTCTGCCCATTTTCCGACGTCACAATCAGCTTTATAT
TGCTACCAGTAACCCAACCAATATCGAAGCCATTGATGCGATCCGCTTTAACAGCAAGCTGAATATTGAAGTGGTGATTG
TTGAGCATGACAAGCTGGAACGGCTGATTGAACAGAACTTTACCGAAGACAGCACCTTTGACTTTGATGAAGACTTTGAT
CTGGAAGTTGGCGACAACGCTCCCGAAACACAAAAAGATGACGATGAGCCACAAGGCGATGAAGCCCCAATCGTCAAATA
TATTAACAAGTTACTGGTCGATGCAATCCGTATGGGCGCCTCGGATTTACACTTCGAGCCTTATGAAAAAAGCTACCGGG
TCCGTTACCGGATTGACGGCGTTTTGCGCCAGATTGCCAATCCTCCGTTACAACTGGCCACACGCCTTGCCTCACGTTTA
AAAGTCATGTCACAAATGGACATCTCGGAAAAACGTATTCCGCAGGATGGCCGGATCAAGCTCAAGCTGTCAAAAAACAA
AGCCATCGACTTCCGTGTCAACTCATTGCCGACCCTGTTTGGAGAAAAAATCGTACTGCGTATCCTCGATCCATCTAGCG
CCATGCTGGGGATTGATGCACTTGGTTATGAACCGGAGCAAAAAGAGCTGTTTATGGAGGCGCTGGACAAACCACAAGGC
ATGTTGCTGATCACCGGCCCGACCGGTTCCGGTAAAACCGTATCTTTATATACCGGCTTAAACATTCTCAACCGCGAAGA
CACCAATATTTCTACCGCCGAAGATCCGGTGGAAATTAACCTGGAAGGCATCAATCAGGTCAACGTCAACCCGAAAGTGG
GTCTGACCTTTGCCGCAGCACTAAAATCATTCCTGCGCCAGGATCCGGATATTGTCATGGTCGGTGAGATCCGTGATCTG
GAAACTGCAGAAATTGCCATTAAAGCCGCACAAACCGGTCACATGGTCATGTCGACGCTGCACACCAACAGTGCACCCGA
AACCCTGACCCGTTTACGCAACATGGGCGTCCCCTCATTTAACATCGCCACCTCGGTGAACCTGGTGATTGCCCAGCGTC
TGGCACGTCGTCTCTGCCAGAAATGCAAGGCCCCGGTGGAGATTCCACGCCAGAGCCTCTTGGAACTCGGTTTTACCGAA
GAAGATTTAAACCAGCCAGAGTTCCAGCTGTATCAGCCGGTTGGCTGCCCGGAATGTCGTGAAGGTTATAAAGGCCGTGT
CGGGGTCTATGAAGTAATGAAAGTCACCCCGGAAATCTCACGCATTATTATGGAAGATGGTAATGCCTTGCAGATTGCAG
CAGCCTCGGCAAAATCCGGGTTTAACAATTTACGCCGTTCAGGATTAATCAAGGTCATGCAGGGGGTCACTTCTTTACAG
GAAGTGAATCGTGTGACCAGCGAATAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.351

100

0.806
  pilB Acinetobacter baylyi ADP1

78.07

100

0.783
  pilB Legionella pneumophila strain ERS1305867

50.97

99.824

0.509
  pilF Neisseria gonorrhoeae MS11

51.429

98.592

0.507
  pilB Vibrio cholerae strain A1552

49.647

99.648

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

49.551

98.063

0.486
  pilB Vibrio campbellii strain DS40M4

50.672

91.725

0.465
  pilF Thermus thermophilus HB27

37.128

100

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

33.821

100

0.366