Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JW980_RS15235 Genome accession   NZ_CP070866
Coordinates   3083322..3085034 (+) Length   570 a.a.
NCBI ID   WP_205667274.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain XY27     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3078322..3090034
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JW980_RS15190 (JW980_15190) nusA 3078906..3080390 (-) 1485 WP_004692706.1 transcription termination factor NusA -
  JW980_RS15195 (JW980_15195) rimP 3080430..3080954 (-) 525 WP_004692708.1 ribosome maturation factor RimP -
  JW980_RS15225 (JW980_15225) secG 3081908..3082237 (-) 330 WP_004692710.1 preprotein translocase subunit SecG -
  JW980_RS15230 (JW980_15230) tpiA 3082251..3083042 (-) 792 WP_205667273.1 triose-phosphate isomerase -
  JW980_RS15235 (JW980_15235) pilB 3083322..3085034 (+) 1713 WP_205667274.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JW980_RS15240 (JW980_15240) pilC 3085065..3086291 (+) 1227 WP_078425747.1 type II secretion system F family protein Machinery gene
  JW980_RS15245 (JW980_15245) pilD 3086291..3087151 (+) 861 WP_058868793.1 prepilin peptidase Machinery gene
  JW980_RS15250 (JW980_15250) coaE 3087160..3087759 (+) 600 WP_058868792.1 dephospho-CoA kinase -
  JW980_RS15255 (JW980_15255) - 3087773..3088690 (-) 918 WP_205667275.1 DMT family transporter -
  JW980_RS15260 (JW980_15260) rlmB 3088723..3089472 (-) 750 WP_004692717.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  JW980_RS15265 (JW980_15265) - 3089615..3089938 (-) 324 WP_004692718.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63604.99 Da        Isoelectric Point: 5.0106

>NTDB_id=541162 JW980_RS15235 WP_205667274.1 3083322..3085034(+) (pilB) [Acinetobacter johnsonii strain XY27]
MSVSNGLPKFTGFIRRLVEDGRMTAENMQQAMLSAKKAQQDIVPYLIENHKLSPQMIAETISLEFGEPVFDLSVYDSVQI
LRDLVEDKLITKHRVLPIYRRGNILYIATSNPTNMDAMDAIRFNSKMNIEAIIVEHPQLEKLIEQNFAQADSFEFSDEDI
DLDLEQDPQHSNDEEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNALPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKDLFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKATVDIPEQSLLEMGF
TSTDIKDPEFKVYQPVGCAECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=541162 JW980_RS15235 WP_205667274.1 3083322..3085034(+) (pilB) [Acinetobacter johnsonii strain XY27]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGACGCATGACTGCTGAAAA
TATGCAGCAGGCAATGCTCTCTGCAAAAAAGGCTCAGCAAGACATCGTTCCTTATCTGATTGAAAATCATAAACTCTCCC
CACAAATGATCGCTGAAACGATTTCTTTAGAGTTTGGCGAGCCTGTTTTTGACTTAAGTGTCTATGATTCTGTACAAATT
CTACGGGATCTTGTCGAAGACAAACTTATCACCAAGCATCGTGTTTTGCCGATTTACCGTCGTGGCAATATCCTCTATAT
CGCTACCAGCAACCCAACCAACATGGATGCGATGGACGCGATTCGCTTCAATTCTAAAATGAATATTGAAGCGATTATTG
TTGAACATCCTCAACTTGAAAAACTAATCGAACAAAATTTCGCGCAAGCCGATAGCTTTGAATTTTCTGATGAAGATATT
GATTTAGACTTAGAGCAAGACCCTCAGCACTCAAATGACGAAGAAGATGATTCACCTCAAGGGGATGAAGCACCGATCGT
CAAATACATCAATAAATTACTGATCGATGCGATTCGTATGGGCGCATCAGATTTACACTTTGAACCTTATGAAAAATCTT
ACCGTGTTCGTTACCGTGTAGATGGGGTTCTGCGTCATATTGCCAGTCCGCCACTACAACTGGCGACTCGTCTTGCCTCA
CGTTTAAAAGTTATGTCACAAATGGACATCTCTGAAAAGCGCGTTCCACAAGATGGCCGCATTAAATTAAAACTGTCAAA
AAGTAAAGCCATCGACTTTCGTGTCAACGCACTTCCAACCTTGTTTGGTGAGAAATTGGTTCTGCGTATTCTCGATCCAT
CCAGTGCTATGCTGGGGATTGACGCTTTAGGTTATGAACCAGATCAAAAAGACCTGTTTATGCAGGCTTTAGACAAACCA
CAAGGCATGTTGTTAATTACTGGCCCGACGGGTTCAGGTAAAACGGTATCGCTATATACAGGTTTGAATATTCTAAATAA
AGAAGACACCAATATTTCAACTGCGGAAGACCCTGTTGAGATTAACTTAGAAGGCATTAATCAGGTTAACGTGAACAATA
AAGTCGGCCTCACCTTCTCTGCTGCACTGAAGTCATTCTTACGTCAGGATCCTGATATTGTGATGGTGGGTGAGATTCGT
GACCTTGAAACCGCTGAAATCGCGATTAAAGCCGCGCAGACAGGTCACATGGTCATGTCGACACTCCACACCAATAGTGC
ACCTGAAACCCTGACCCGCTTACGCAATATGGGGGTTCCATCTTTTAACATTGCGACATCGGTCAATTTGGTAATTGCAC
AACGTTTAGCACGCCGCTTATGTAGCCAATGCAAAGCCACTGTCGATATTCCTGAACAAAGCTTGCTCGAAATGGGCTTT
ACTTCCACAGATATCAAAGATCCAGAATTCAAAGTTTATCAACCGGTGGGATGTGCTGAGTGCCGTGAAGGCTATAAAGG
ACGTGTTGGTATTTATGAAGTCATGAAAGTCACGCCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCTTACAGA
TTGCGGAAGCATCTGCAAAGTTAGGATTCAACAATTTACGTCGTTCAGGTTTACTCAAGGTTATGCAGGGGGTCACTTCC
TTACAAGAAGTGAATCGTGTAACCAGCGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.877

100

0.809
  pilB Acinetobacter baylyi ADP1

76.842

100

0.768
  pilB Legionella pneumophila strain ERS1305867

53.933

93.684

0.505
  pilF Neisseria gonorrhoeae MS11

49.644

98.596

0.489
  pilB Vibrio cholerae strain A1552

48.325

99.474

0.481
  pilB Vibrio campbellii strain DS40M4

48.128

98.421

0.474
  pilB Vibrio parahaemolyticus RIMD 2210633

50.677

90.702

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

33.926

100

0.368
  pilF Thermus thermophilus HB27

36.602

100

0.367