Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   A0J47_RS02640 Genome accession   NZ_CP070624
Coordinates   546747..548474 (+) Length   575 a.a.
NCBI ID   WP_374079738.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. damselae strain RM-71     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 541747..553474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A0J47_RS02620 (A0J47_002620) pilM 543976..544974 (+) 999 WP_068945319.1 type IV pilus biogenesis protein PilM -
  A0J47_RS02625 (A0J47_002625) - 544971..545612 (+) 642 WP_068945318.1 PilN domain-containing protein -
  A0J47_RS02630 (A0J47_002630) - 545609..546199 (+) 591 WP_068945317.1 type 4a pilus biogenesis protein PilO -
  A0J47_RS02635 (A0J47_002635) - 546208..546723 (+) 516 WP_036764704.1 pilus assembly protein PilP -
  A0J47_RS02640 (A0J47_002640) pilQ 546747..548474 (+) 1728 WP_374079738.1 type IV pilus secretin PilQ Machinery gene
  A0J47_RS02645 (A0J47_002645) aroK 548804..549322 (+) 519 WP_036765246.1 shikimate kinase AroK -
  A0J47_RS02650 (A0J47_002650) aroB 549352..550431 (+) 1080 WP_005302661.1 3-dehydroquinate synthase -
  A0J47_RS02655 (A0J47_002655) - 550446..551954 (+) 1509 WP_068945316.1 SPOR domain-containing protein -
  A0J47_RS02660 (A0J47_002660) dam 552062..552904 (+) 843 WP_065172417.1 adenine-specific DNA-methyltransferase -
  A0J47_RS02665 (A0J47_002665) - 552888..553085 (-) 198 WP_036764707.1 DUF2970 domain-containing protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62724.21 Da        Isoelectric Point: 4.9029

>NTDB_id=540932 A0J47_RS02640 WP_374079738.1 546747..548474(+) (pilQ) [Photobacterium damselae subsp. damselae strain RM-71]
MKRMLSVSLGWQRCWVVMLCCWLGLSQAAIAAGMNIVNNQITDLDFKRDNRGRGVLTVQLAKPQSLVDMRRSGKQLHIDI
FDTAIKDDMLYVLDVVDFATVVRSIETFRTEKGSKLILNMAGVFDYDYQLKGSRLEVVLNAQKKKASSTAKAKQGKIDYK
GKPISINFQDVPVRNVLQLVAEYNDFNLVVSDTVQGNVTLRLDDVPWPQVLDIILQSKGLDKRVQGSVVLIAPKSELATN
ERQEMEAKRKEDELASLRSELIQINYAKAGDIADLLLGTKDGVSMLSQRGSLHVDERTNAIIIKDIPESIDSIEEIITAL
DIPVKQVQIEARIVTVDEGDIDELGVRWGVVNQNGSTTIGGSIEGNLASSGLLGDDATVDDFLNVNLGAITPGASSIAFQ
VAKLGDILLDLELSALQAESKAEIISSPRLVTTNKKAAYIEQGTEIPYLEAASSGAVSVDFKKAVLSLMVTPQITPDDKL
VLDLLVTQDKVGETVVTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIYQHQVNNSVRKVPLLGDIPGLGVLFRHKLQEMG
KRELLIFVTPKIITQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=540932 A0J47_RS02640 WP_374079738.1 546747..548474(+) (pilQ) [Photobacterium damselae subsp. damselae strain RM-71]
ATGAAAAGGATGTTATCGGTCAGCTTGGGTTGGCAACGTTGTTGGGTAGTTATGTTGTGCTGTTGGCTTGGTCTCTCCCA
AGCTGCCATTGCTGCTGGCATGAATATTGTGAATAACCAAATCACCGACTTAGATTTTAAGCGTGATAACCGTGGACGAG
GCGTGCTCACTGTTCAGTTAGCCAAACCTCAATCTTTGGTTGATATGCGCCGTTCAGGAAAGCAATTACACATCGATATC
TTTGATACCGCGATTAAAGATGACATGTTGTATGTACTCGATGTGGTCGACTTTGCAACTGTTGTTCGTTCGATTGAAAC
ATTTCGCACCGAGAAAGGCAGCAAACTTATTCTCAATATGGCTGGCGTGTTTGATTATGATTACCAATTAAAAGGCTCTC
GTCTTGAAGTTGTTTTGAACGCCCAGAAAAAGAAAGCCAGCAGTACAGCCAAGGCTAAGCAAGGCAAAATTGATTATAAA
GGTAAGCCCATCTCCATTAACTTCCAAGATGTTCCTGTGCGTAACGTATTGCAGTTGGTTGCCGAATATAATGATTTTAA
TCTCGTGGTTTCCGATACTGTGCAGGGTAATGTGACCTTACGTCTTGATGATGTGCCTTGGCCACAAGTGCTCGATATTA
TTTTGCAATCAAAAGGCTTGGATAAACGAGTTCAAGGTTCTGTGGTACTGATTGCGCCAAAATCTGAGTTGGCAACCAAT
GAGCGTCAAGAGATGGAAGCCAAACGTAAAGAAGATGAGTTAGCTTCGCTTCGTTCGGAGCTGATTCAAATTAATTACGC
TAAAGCAGGAGATATCGCAGACCTTTTACTCGGGACTAAAGATGGAGTCAGTATGCTATCTCAACGCGGGTCTTTGCATG
TTGATGAGCGTACTAATGCCATAATCATTAAAGATATTCCTGAGAGTATTGATAGCATTGAAGAGATTATCACGGCACTA
GATATTCCCGTTAAACAGGTTCAGATAGAAGCTCGTATTGTTACTGTTGATGAAGGGGATATTGACGAGTTAGGGGTACG
TTGGGGTGTCGTTAATCAAAACGGTAGTACAACCATTGGGGGCTCAATTGAAGGTAACTTGGCCAGTTCGGGCTTATTGG
GTGATGACGCGACTGTTGATGATTTCTTAAATGTAAATTTAGGTGCAATTACTCCTGGGGCATCGAGTATTGCATTTCAG
GTGGCAAAACTGGGCGATATTTTGCTCGATCTTGAACTATCCGCTCTACAGGCAGAATCGAAAGCAGAGATTATTTCTAG
TCCTCGATTAGTAACTACCAATAAAAAAGCGGCTTACATCGAACAAGGTACAGAGATCCCTTATTTAGAAGCGGCATCTA
GTGGTGCGGTATCGGTAGACTTTAAAAAGGCCGTATTGAGTTTGATGGTCACACCACAAATTACCCCTGATGATAAATTG
GTGCTCGATCTGCTGGTAACGCAAGATAAAGTAGGGGAAACAGTTGTTACCGGAACCGGAGAAGCGGTCGCTATCGATAC
TCAACGAATTGGCACGCAAGTTCTGGTCAATAATGGCGAGACTGTAGTCTTAGGGGGTATCTATCAGCATCAGGTGAATA
ATTCTGTCCGTAAAGTCCCTTTGTTGGGCGATATTCCAGGATTGGGCGTATTGTTCCGTCATAAGCTGCAAGAGATGGGC
AAGCGTGAACTGTTGATTTTCGTCACACCCAAAATTATCACCCAGTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(186-234)

Bacterial type II/III secretion system short domain (Secretin_N)

(260-326)

Bacterial type II and III secretion system protein (Secretin)

(415-573)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

61.296

99.304

0.609
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

61.296

99.304

0.609
  pilQ Vibrio campbellii strain DS40M4

61.111

97.043

0.593
  pilQ Legionella pneumophila strain Lp02

42.831

94.609

0.405
  pilQ Legionella pneumophila strain ERS1305867

42.831

94.609

0.405
  pilQ Pseudomonas aeruginosa PAK

41.103

97.739

0.402
  comQ Acinetobacter baylyi ADP1

40.212

98.609

0.397
  pilQ Acinetobacter baumannii D1279779

39.138

100

0.395
  pilQ Acinetobacter baumannii strain A118

39.138

100

0.395