Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JWZ97_RS06515 Genome accession   NZ_CP070491
Coordinates   1572117..1573832 (+) Length   571 a.a.
NCBI ID   WP_205433987.1    Uniprot ID   A0A974VY08
Organism   Methylococcus sp. EFPC2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1567117..1578832
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JWZ97_RS06495 (JWZ97_06495) - 1567203..1568111 (+) 909 WP_205433984.1 alpha/beta fold hydrolase -
  JWZ97_RS06500 (JWZ97_06500) pgaD 1568090..1568524 (-) 435 WP_205433985.1 poly-beta-1,6-N-acetyl-D-glucosamine biosynthesis protein PgaD -
  JWZ97_RS06505 (JWZ97_06505) pgaC 1568505..1569917 (-) 1413 WP_240342514.1 poly-beta-1,6-N-acetyl-D-glucosamine synthase -
  JWZ97_RS06510 (JWZ97_06510) pgaB 1569957..1571846 (-) 1890 WP_205433986.1 poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB -
  JWZ97_RS06515 (JWZ97_06515) pilB 1572117..1573832 (+) 1716 WP_205433987.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JWZ97_RS06520 (JWZ97_06520) pilC 1573836..1575050 (+) 1215 WP_205433988.1 type II secretion system F family protein Machinery gene
  JWZ97_RS06525 (JWZ97_06525) pilD 1575067..1575945 (+) 879 WP_205433989.1 A24 family peptidase Machinery gene
  JWZ97_RS06530 (JWZ97_06530) coaE 1575949..1576548 (+) 600 WP_205433990.1 dephospho-CoA kinase -
  JWZ97_RS06535 (JWZ97_06535) zapD 1576571..1577392 (+) 822 WP_240342515.1 cell division protein ZapD -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63360.06 Da        Isoelectric Point: 6.0865

>NTDB_id=540175 JWZ97_RS06515 WP_205433987.1 1572117..1573832(+) (pilB) [Methylococcus sp. EFPC2]
MASLAPKLQLSGLARSLVKEELLTEVQAQTLFEEAHQKKIHFVSHLVANKILDARSIAVTVSREFGLPLFDLDAMMTDTI
PVKLVSERLIRQHHALPLFKRGNRLYLAVSDPTNFQGLDEIKFHTRLSSDCVVVEEDKLSKLIDHALEAADTSMKDLLDT
DLDNLQISGGDEIEARADSDASDVDEAPIVRFVNKILLDAIKKGASDIHIEPYEKNFRIRFRHDGMLHEVTSPPANLASR
IAARLKVMSRMDISERRIPQDGRIKMALSRTRAIDFRVNTCPTLFGEKIVLRILDPTSAQIGIERLGFEPEQQENFLRAI
KKPYGMILVTGPTGSGKTVTLYTALNILNEPDVNISTAEDPVEITVPGINQVNVNVKTGLTFAEALRAFLRQDPDIIMVG
EIRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLNRLMQMGIPSFNIASSVLLIMAQRLARRLCEHCKQEVKLPPEALLD
AGFEENEMEGLTLYGPTGCDKCVKGYKGRVGIYQVMPISESINRIILEGGNVMQLAAQAEREGISDLRRSGLKKVKAGIT
SLEEIDRVTRD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=540175 JWZ97_RS06515 WP_205433987.1 1572117..1573832(+) (pilB) [Methylococcus sp. EFPC2]
ATGGCCTCACTGGCGCCTAAATTACAACTCAGCGGTCTTGCCAGAAGCCTGGTCAAAGAAGAATTGCTGACGGAAGTGCA
GGCGCAGACCTTGTTTGAAGAAGCGCACCAGAAAAAGATTCACTTCGTCTCCCATCTGGTCGCCAACAAGATCTTGGATG
CCCGTAGCATAGCGGTCACCGTTTCCCGAGAATTCGGCCTGCCCTTGTTCGATCTCGATGCCATGATGACCGATACGATC
CCGGTCAAACTGGTCAGCGAGCGGCTGATCCGCCAGCATCACGCCCTACCGCTGTTCAAACGGGGCAACCGGCTCTACCT
GGCCGTTTCGGACCCGACCAATTTCCAGGGACTTGACGAAATCAAGTTCCATACGCGCCTGTCATCCGACTGCGTGGTCG
TCGAAGAAGACAAGTTGTCCAAGCTGATAGACCACGCGCTGGAAGCCGCCGACACGTCCATGAAGGACCTGCTGGACACG
GATCTGGACAATCTGCAGATCTCGGGCGGCGACGAGATCGAGGCCCGAGCGGACAGCGACGCCAGTGATGTTGACGAAGC
TCCCATTGTGCGTTTCGTCAACAAGATACTCCTGGACGCCATTAAAAAAGGGGCTTCAGACATCCATATCGAGCCCTACG
AAAAAAATTTCCGCATCCGTTTCCGCCACGACGGCATGCTGCACGAGGTCACCAGTCCGCCGGCCAATCTGGCCTCGCGT
ATCGCGGCCCGCCTCAAGGTGATGTCGCGCATGGACATCTCCGAACGGCGCATCCCGCAGGACGGCCGCATTAAAATGGC
CTTGTCCCGCACCCGGGCCATCGACTTCCGCGTCAATACCTGCCCCACGCTGTTCGGCGAAAAAATCGTGTTGCGCATCC
TGGATCCGACCAGTGCGCAGATCGGTATCGAAAGGCTGGGCTTCGAACCGGAGCAGCAGGAGAACTTCCTGCGCGCGATC
AAGAAGCCCTACGGCATGATATTGGTCACCGGTCCGACCGGCAGCGGCAAGACCGTCACCTTGTATACGGCGTTGAACAT
CCTGAACGAGCCGGACGTGAATATCTCCACCGCCGAGGATCCGGTCGAAATCACGGTGCCCGGCATCAACCAGGTCAATG
TCAACGTCAAGACCGGACTCACCTTTGCCGAGGCTCTGCGCGCTTTCCTGCGCCAGGATCCGGATATCATCATGGTGGGC
GAAATACGCGATTTGGAAACCGCCGAAATCGCCGTCAAGGCCGCCCAAACCGGCCACTTGGTGCTTTCGACGCTGCACAC
CAACGACGCGCCTCAGACGCTCAACCGGCTCATGCAAATGGGCATCCCTTCATTCAATATCGCATCCTCGGTCCTCCTGA
TCATGGCTCAGCGCCTGGCCCGCCGCCTGTGCGAACACTGCAAGCAGGAAGTCAAGCTTCCACCGGAAGCCCTGCTGGAT
GCGGGATTCGAAGAAAATGAAATGGAAGGTCTGACGCTATACGGCCCCACTGGCTGCGACAAATGCGTCAAGGGATACAA
GGGGCGGGTGGGCATTTACCAGGTGATGCCCATATCGGAAAGCATCAACCGCATCATACTCGAGGGCGGTAACGTCATGC
AGTTGGCGGCGCAAGCCGAGCGCGAAGGGATCTCCGACCTGAGGCGCTCAGGCCTGAAAAAAGTGAAAGCCGGCATTACC
AGCCTGGAAGAAATCGACCGCGTGACACGGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

57.739

100

0.581
  pilB Acinetobacter baumannii D1279779

53.927

100

0.541
  pilB Acinetobacter baylyi ADP1

53.136

100

0.534
  pilF Neisseria gonorrhoeae MS11

48.936

98.774

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

48.496

98.949

0.48
  pilB Vibrio cholerae strain A1552

48.668

98.599

0.48
  pilB Vibrio campbellii strain DS40M4

47.78

98.599

0.471
  pilF Thermus thermophilus HB27

40.734

95.447

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.731

91.068

0.38
  pilB/pilB1 Synechocystis sp. PCC 6803

40.154

90.718

0.364