Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q91_RS01930 Genome accession   NC_018697
Coordinates   395144..396853 (-) Length   569 a.a.
NCBI ID   WP_015005195.1    Uniprot ID   K0C1P2
Organism   Cycloclasticus sp. P1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 390144..401853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q91_RS01900 (Q91_0377) - 390457..391392 (+) 936 WP_015005189.1 Nudix family hydrolase -
  Q91_RS01905 (Q91_0378) yacG 391403..391591 (-) 189 WP_015005190.1 DNA gyrase inhibitor YacG -
  Q91_RS01910 (Q91_0379) zapD 391593..392360 (-) 768 WP_238538349.1 cell division protein ZapD -
  Q91_RS01915 (Q91_0380) coaE 392401..393006 (-) 606 WP_015005192.1 dephospho-CoA kinase -
  Q91_RS01920 (Q91_0381) pilD 393013..393888 (-) 876 WP_015005193.1 A24 family peptidase Machinery gene
  Q91_RS01925 (Q91_0382) pilC 393918..395138 (-) 1221 WP_015005194.1 type II secretion system F family protein Machinery gene
  Q91_RS01930 (Q91_0383) pilB 395144..396853 (-) 1710 WP_015005195.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q91_RS01935 (Q91_0384) - 397151..397903 (+) 753 WP_015005196.1 glycosyltransferase family 2 protein -
  Q91_RS01940 (Q91_0385) - 397911..399263 (+) 1353 WP_015005197.1 flippase -
  Q91_RS01945 (Q91_0386) - 399339..400745 (+) 1407 WP_015005198.1 hypothetical protein -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63112.65 Da        Isoelectric Point: 4.8588

>NTDB_id=53782 Q91_RS01930 WP_015005195.1 395144..396853(-) (pilB) [Cycloclasticus sp. P1]
MAVSGEKFYLGGLARRLVLDGLITEDVALKYSEEAYKKKRPFVSFLVEKKVLDSQIIALSASEEFGVPLFDITALSKDVI
PKGIVDEKLVRKHHALPIFKRGNKLFVAVSDPTNLQALDEIKFQTRMSTEAILVEEDKLARVIDTAMDEEDTSMSDLLDD
DLENIDFDDPDAEPVDDIDSEIDDAPVVRFVNKILLDAIKKGASDIHFEPYEKSFRIRTRQDGILKEVASPPINISNKLI
SRIKVMSRMNISERRIPQDGRIKMALSKKRAVDFRVNTCPTLYGEKIVLRILDPTSAQVGIEQLGFEPEQKEAFLTAIQK
PYGMILVTGPTGSGKTVSLYTALNLLNDPNINISTAEDPVEITVSGINQVNVNPKTGLTFAEALRAFLRQDPDIIMVGEI
RDLETAEISVKAAQTGHLVLSTLHTNDAPQTLNRLNQMGVPPYNIAASVLLIMAQRLARKLCDKCKKEETLPTELLIKEG
FSPEEAERLTTYKAVGCDHCTNGYKGRIGIFQVMPVSEAMENLIIKGGLTTELEEQAAQEGVLTLRESGFKKVIAGITSI
EEINRITKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=53782 Q91_RS01930 WP_015005195.1 395144..396853(-) (pilB) [Cycloclasticus sp. P1]
ATGGCTGTATCCGGCGAAAAATTTTATTTAGGTGGCTTAGCAAGACGATTGGTCTTAGACGGTCTAATTACTGAAGACGT
TGCACTCAAATATTCTGAGGAGGCTTATAAAAAGAAAAGACCTTTTGTTAGCTTTCTTGTAGAGAAAAAAGTTTTAGATA
GTCAAATTATTGCTCTTTCTGCTTCTGAAGAGTTTGGCGTTCCATTATTTGATATTACTGCACTGAGCAAGGATGTTATT
CCCAAAGGTATTGTTGACGAAAAATTAGTTCGTAAACACCATGCATTACCGATATTTAAACGTGGCAATAAGCTGTTTGT
TGCAGTATCTGACCCGACTAACCTTCAAGCGCTTGATGAAATAAAGTTCCAAACAAGGATGAGTACCGAAGCCATTCTTG
TTGAAGAAGATAAGTTAGCACGTGTTATTGACACAGCTATGGATGAAGAAGATACATCCATGAGTGACTTATTAGATGAC
GATCTTGAAAATATTGATTTCGATGATCCCGATGCAGAGCCCGTGGATGATATTGACAGTGAGATTGATGATGCACCGGT
TGTGCGGTTTGTTAACAAAATCCTATTAGATGCTATTAAAAAAGGCGCGTCGGATATTCATTTTGAGCCGTATGAGAAAA
GTTTTAGAATCCGAACACGGCAAGATGGTATTTTGAAAGAAGTTGCTTCACCACCAATTAATATCTCAAACAAACTGATA
TCACGAATTAAAGTGATGTCGCGGATGAATATTTCAGAACGCCGTATTCCACAGGACGGTCGTATTAAAATGGCTCTTTC
TAAAAAAAGGGCCGTTGATTTTCGTGTTAACACCTGCCCTACTTTATACGGTGAGAAAATTGTACTGCGTATTTTGGATC
CGACCAGCGCACAAGTGGGTATCGAACAGCTAGGGTTTGAGCCTGAGCAAAAAGAAGCCTTCCTAACGGCTATTCAGAAA
CCTTATGGCATGATTCTCGTCACCGGCCCAACCGGTAGTGGTAAGACCGTTAGCCTTTATACCGCGCTTAACTTATTAAA
CGACCCAAACATCAATATTTCAACCGCTGAAGACCCCGTAGAAATTACCGTATCGGGTATTAACCAAGTTAATGTTAACC
CTAAAACCGGTCTAACTTTTGCTGAAGCGCTTCGAGCTTTTTTACGCCAAGATCCAGATATAATCATGGTCGGTGAGATT
CGAGACCTTGAAACCGCTGAAATTTCTGTTAAAGCCGCACAAACCGGTCACCTTGTTCTATCTACATTACACACAAACGA
TGCCCCACAAACCTTAAACCGTTTAAACCAAATGGGCGTTCCACCCTACAATATTGCAGCATCTGTGTTATTAATTATGG
CGCAACGTTTAGCAAGAAAACTATGCGATAAATGTAAAAAAGAAGAGACATTACCAACAGAGTTATTAATCAAAGAAGGC
TTTAGCCCTGAAGAAGCTGAAAGATTAACAACTTACAAGGCTGTAGGCTGCGATCATTGCACTAACGGCTATAAAGGGCG
TATTGGCATTTTCCAGGTGATGCCTGTTTCTGAAGCCATGGAAAACCTTATCATTAAAGGTGGTTTAACAACTGAACTTG
AAGAACAAGCTGCACAAGAGGGCGTTTTAACACTCAGGGAGTCTGGCTTTAAAAAGGTCATCGCTGGTATTACCAGCATA
GAAGAAATAAACCGAATCACTAAAGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K0C1P2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.478

100

0.561
  pilB Acinetobacter baylyi ADP1

51.779

98.77

0.511
  pilB Acinetobacter baumannii D1279779

51.337

98.594

0.506
  pilF Neisseria gonorrhoeae MS11

48.587

99.473

0.483
  pilB Vibrio cholerae strain A1552

49.715

92.619

0.46
  pilB Vibrio parahaemolyticus RIMD 2210633

48.099

92.443

0.445
  pilB Vibrio campbellii strain DS40M4

50.932

84.886

0.432
  pilF Thermus thermophilus HB27

39.963

94.552

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

40.347

91.037

0.367

Multiple sequence alignment