Detailed information NTG0053375

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: HMPREF1038_RS10035
• Genome accession: NC_018630
• Coordinates: 1861190..1861651 (-)
• Length: 153 a.a.
• NCBI ID: WP_000250531.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae gamPNI0373
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1856190..1866651

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HMPREF1038_RS10000 (HMPREF1038_02036)	-	1856494..1857318 (-)	825	WP_000727904.1	membrane protein insertase YidC	-
HMPREF1038_RS10005 (HMPREF1038_02037)	rnpA	1857293..1857664 (-)	372	WP_000739246.1	ribonuclease P protein component	-
HMPREF1038_RS13455 (HMPREF1038_02038)	-	1857681..1857812 (-)	132	WP_000768904.1	hypothetical protein	-
HMPREF1038_RS10010 (HMPREF1038_02039)	-	1857813..1859003 (-)	1191	WP_000167766.1	acetate kinase	-
HMPREF1038_RS10015 (HMPREF1038_02040)	comYH	1859054..1860007 (-)	954	WP_000345116.1	class I SAM-dependent methyltransferase	Machinery gene
HMPREF1038_RS12125	-	1860068..1860662 (-)	595	Protein_1886	class I SAM-dependent methyltransferase	-
HMPREF1038_RS10030 (HMPREF1038_02044)	comGG/cglG	1860799..1861212 (-)	414	WP_000265631.1	competence type IV pilus minor pilin ComGG	Machinery gene
HMPREF1038_RS10035 (HMPREF1038_02045)	comGF/cglF	1861190..1861651 (-)	462	WP_000250531.1	competence type IV pilus minor pilin ComGF	Machinery gene
HMPREF1038_RS10040 (HMPREF1038_02046)	comGE/cglE	1861614..1861916 (-)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
HMPREF1038_RS10045 (HMPREF1038_02047)	comGD/cglD	1861879..1862283 (-)	405	WP_000588020.1	competence type IV pilus minor pilin ComGD	Machinery gene
HMPREF1038_RS10050 (HMPREF1038_02048)	comGC/cglC	1862276..1862602 (-)	327	WP_000738633.1	comG operon protein ComGC	Machinery gene
HMPREF1038_RS10055 (HMPREF1038_02049)	comGB/cglB	1862604..1863620 (-)	1017	WP_001844679.1	competence type IV pilus assembly protein ComGB	Machinery gene
HMPREF1038_RS10060 (HMPREF1038_02050)	comGA/cglA/cilD	1863568..1864509 (-)	942	WP_001844680.1	competence type IV pilus ATPase ComGA	Machinery gene
HMPREF1038_RS10065 (HMPREF1038_02051)	-	1864585..1864950 (-)	366	WP_000286415.1	DUF1033 family protein	-
HMPREF1038_RS10070 (HMPREF1038_02052)	-	1865101..1866159 (-)	1059	WP_000649471.1	zinc-dependent alcohol dehydrogenase family protein	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17849.37 Da
• Isoelectric Point: 6.4832

>NTDB_id=53375 HMPREF1038_RS10035 WP_000250531.1 1861190..1861651(-) (comGF/cglF) [Streptococcus pneumoniae gamPNI0373]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide

• Length: 462 bp

>NTDB_id=53375 HMPREF1038_RS10035 WP_000250531.1 1861190..1861651(-) (comGF/cglF) [Streptococcus pneumoniae gamPNI0373]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGTAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	95.425	100	0.954
comGF/cglF	Streptococcus pneumoniae D39	95.425	100	0.954
comGF/cglF	Streptococcus pneumoniae R6	95.425	100	0.954
comGF/cglF	Streptococcus pneumoniae TIGR4	95.425	100	0.954
comGF/cglF	Streptococcus mitis SK321	92.81	100	0.928
comGF/cglF	Streptococcus mitis NCTC 12261	91.503	100	0.915