Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   JNO62_RS03000 Genome accession   NZ_CP069245
Coordinates   649440..650705 (-) Length   421 a.a.
NCBI ID   WP_019718222.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain PeaFJ1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 644440..655705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNO62_RS02970 (JNO62_02960) - 645511..646395 (+) 885 WP_011002738.1 ATP-binding protein -
  JNO62_RS02975 (JNO62_02965) - 646373..646834 (+) 462 WP_011002737.1 NUDIX domain-containing protein -
  JNO62_RS02980 (JNO62_02970) yacG 646827..647042 (-) 216 WP_011002736.1 DNA gyrase inhibitor YacG -
  JNO62_RS02985 (JNO62_02975) zapD 647053..647811 (-) 759 WP_016723794.1 cell division protein ZapD -
  JNO62_RS02990 (JNO62_02980) coaE 647929..648543 (-) 615 WP_011002734.1 dephospho-CoA kinase -
  JNO62_RS02995 (JNO62_02985) - 648556..649437 (-) 882 WP_029240316.1 prepilin peptidase -
  JNO62_RS03000 (JNO62_02990) pilC 649440..650705 (-) 1266 WP_019718222.1 type II secretion system F family protein Machinery gene
  JNO62_RS03005 (JNO62_02995) pilF 650750..652477 (-) 1728 WP_071013048.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JNO62_RS03010 (JNO62_03000) - 652725..654002 (-) 1278 WP_016723792.1 HlyC/CorC family transporter -
  JNO62_RS03020 (JNO62_03010) ispB 654290..655258 (-) 969 WP_111374599.1 octaprenyl diphosphate synthase -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 46555.83 Da        Isoelectric Point: 10.3648

>NTDB_id=533139 JNO62_RS03000 WP_019718222.1 649440..650705(-) (pilC) [Ralstonia pseudosolanacearum strain PeaFJ1]
MATRAPTANRSAAASARKTAATKAPTQYIFEWEGKDRKGKIFKGEMRAESITEVNAVLRKQGLSITKSKRRRAARGKKIV
PKDIAYFTRQLSTMLKAGVPLLQSVDIIARGHANPNFTQLLTEIRVDIESGSSMAQAFRRHPQHFDSLYCNLIDAGEQGG
ILDALLERLSMYMEKSIALKAQIKSAMIYPISVLTVAFLVTVVLMIFVVPAFKNVFASFGATLPAPTLVVMGISDFFVKW
WYIIIFTPVVGIFLYVRAFKRSEKVQRNTHKAILKMPIFGSIIRKATIARWTRTLATMFSAGTPLVESMESVAGAAGNWV
YYDATMEIQQAVRIGTSLTNAMQATHVFDNMVLQMTQIGEESGALDNMLLKVAEFYEREVDDAVANISALIEPLIIVILG
VLIGGMVVAMYLPIFKLGQVV

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=533139 JNO62_RS03000 WP_019718222.1 649440..650705(-) (pilC) [Ralstonia pseudosolanacearum strain PeaFJ1]
ATGGCCACACGAGCACCGACCGCGAACCGGTCCGCCGCCGCATCGGCACGCAAGACCGCCGCCACGAAGGCGCCCACGCA
GTACATCTTCGAGTGGGAAGGCAAGGACCGCAAAGGCAAGATCTTCAAGGGCGAGATGCGGGCGGAGAGCATCACCGAAG
TCAATGCGGTGCTGCGCAAGCAAGGGCTGTCGATCACCAAGTCCAAGCGTCGGCGTGCCGCGCGCGGCAAGAAGATCGTA
CCCAAGGACATCGCCTACTTCACGCGCCAGCTGTCGACCATGCTGAAAGCGGGCGTGCCGCTGCTGCAATCGGTCGACAT
CATCGCGCGCGGGCACGCCAACCCGAACTTCACCCAGCTGCTGACGGAAATCCGTGTCGACATCGAGTCGGGCAGCAGCA
TGGCGCAGGCCTTCCGCCGCCATCCGCAGCACTTCGATTCGCTGTACTGCAACCTGATCGACGCCGGTGAGCAGGGCGGT
ATTCTCGACGCGCTGCTCGAGCGGCTGTCGATGTACATGGAAAAGTCGATCGCGCTGAAGGCGCAGATCAAGTCGGCCAT
GATCTATCCGATCTCGGTGCTGACGGTGGCTTTCCTGGTCACGGTGGTGCTGATGATCTTCGTGGTGCCGGCGTTCAAGA
ACGTGTTCGCCAGCTTCGGCGCCACGCTGCCGGCCCCGACGCTGGTGGTGATGGGCATCTCGGACTTCTTCGTGAAGTGG
TGGTACATCATCATCTTCACGCCCGTCGTCGGCATCTTCCTGTATGTGCGGGCTTTCAAGCGCTCGGAGAAGGTGCAGCG
CAACACGCACAAGGCCATCCTGAAGATGCCGATCTTCGGCAGCATCATCCGCAAGGCCACCATCGCGCGCTGGACGCGTA
CGCTGGCAACCATGTTCTCGGCCGGGACACCGCTGGTGGAGTCGATGGAATCCGTCGCGGGCGCCGCCGGCAACTGGGTC
TACTATGACGCCACCATGGAGATCCAGCAGGCGGTGCGCATCGGTACCAGCCTCACCAACGCCATGCAGGCGACCCATGT
GTTCGACAACATGGTGCTGCAGATGACGCAGATCGGCGAGGAATCCGGCGCGCTGGACAACATGCTGCTGAAGGTGGCCG
AATTCTACGAGCGCGAGGTCGATGACGCGGTGGCCAACATCTCCGCCCTGATCGAGCCGCTCATCATCGTGATCCTGGGC
GTGCTGATCGGGGGCATGGTGGTTGCCATGTACCTGCCGATCTTCAAGCTGGGCCAAGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

50.495

95.962

0.485
  pilG Neisseria gonorrhoeae MS11

50.125

94.774

0.475
  pilG Neisseria meningitidis 44/76-A

49.875

94.774

0.473
  pilC Acinetobacter baumannii D1279779

47.912

96.675

0.463
  pilC Acinetobacter baylyi ADP1

46.683

96.675

0.451
  pilC Legionella pneumophila strain ERS1305867

45.926

96.2

0.442
  pilC Vibrio campbellii strain DS40M4

39.604

95.962

0.38
  pilC Vibrio cholerae strain A1552

39.75

95.012

0.378