Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   JNO62_RS03005 Genome accession   NZ_CP069245
Coordinates   650750..652477 (-) Length   575 a.a.
NCBI ID   WP_071013048.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain PeaFJ1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 645750..657477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNO62_RS02975 (JNO62_02965) - 646373..646834 (+) 462 WP_011002737.1 NUDIX domain-containing protein -
  JNO62_RS02980 (JNO62_02970) yacG 646827..647042 (-) 216 WP_011002736.1 DNA gyrase inhibitor YacG -
  JNO62_RS02985 (JNO62_02975) zapD 647053..647811 (-) 759 WP_016723794.1 cell division protein ZapD -
  JNO62_RS02990 (JNO62_02980) coaE 647929..648543 (-) 615 WP_011002734.1 dephospho-CoA kinase -
  JNO62_RS02995 (JNO62_02985) - 648556..649437 (-) 882 WP_029240316.1 prepilin peptidase -
  JNO62_RS03000 (JNO62_02990) pilC 649440..650705 (-) 1266 WP_019718222.1 type II secretion system F family protein Machinery gene
  JNO62_RS03005 (JNO62_02995) pilF 650750..652477 (-) 1728 WP_071013048.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JNO62_RS03010 (JNO62_03000) - 652725..654002 (-) 1278 WP_016723792.1 HlyC/CorC family transporter -
  JNO62_RS03020 (JNO62_03010) ispB 654290..655258 (-) 969 WP_111374599.1 octaprenyl diphosphate synthase -
  JNO62_RS03025 (JNO62_03015) rplU 655544..655855 (+) 312 WP_011002728.1 50S ribosomal protein L21 -
  JNO62_RS03030 (JNO62_03020) rpmA 655893..656153 (+) 261 WP_003271630.1 50S ribosomal protein L27 -
  JNO62_RS03035 (JNO62_03025) obgE 656330..657430 (+) 1101 WP_011002727.1 GTPase ObgE -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63740.58 Da        Isoelectric Point: 7.1546

>NTDB_id=533140 JNO62_RS03005 WP_071013048.1 650750..652477(-) (pilF) [Ralstonia pseudosolanacearum strain PeaFJ1]
MTLGLSLAQSRRIAPTLLAQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPPVLSANRHF
QQLRLLPLGRRDNRIIVATSDPSDPKPLEELRQKMNVAIETVIVEHDKLVRQLSLSNEAPAEIKSLIPKQETTQIEYDPG
AAAAVRRTPTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKEMVERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNKLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPKETL
LDAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILAHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=533140 JNO62_RS03005 WP_071013048.1 650750..652477(-) (pilF) [Ralstonia pseudosolanacearum strain PeaFJ1]
ATGACTCTCGGACTCTCTCTTGCGCAGAGCCGGCGAATTGCGCCGACATTACTCGCCCAGCTGGAACAAAGCGCCCGCGA
AAAGCGCACGCAGCTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCTCATGATCTCGCCGTCTTCGTGGCGGGCA
AATACCAGATGCCGCTGCTGGACCTGGCGCAATACAAGCTCGACACCGTGCCGCCCGTGCTGTCGGCCAACAGGCATTTT
CAGCAGTTGCGCCTGTTGCCGCTGGGCCGGCGTGACAACCGCATCATCGTGGCCACTTCGGACCCGAGCGACCCCAAGCC
GCTCGAGGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAGCTGA
GCCTCTCCAACGAGGCACCGGCCGAGATCAAGTCGCTCATCCCCAAGCAGGAAACCACGCAGATCGAATACGACCCGGGT
GCGGCCGCGGCGGTCCGCCGCACCCCGACCGACGGCATCGACGATGCGCCGGTGGTGCGCTTCCTGCAGAAGCTGTTTAC
CGAGGCGCTGCTGCGCGGCACCTCCGACTTGCATTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGCATCGACG
GCATCCTCGAGCAGGTCGCGCAGCCGCCGCTGGACATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCGCGCCTG
GACATATCGGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTCCTGATCACCGTTCCGAAAGACAAGAACGACAAGGA
CAACAAGGAGATGGTCGAGCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTCTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCGACCGAGCGGCTCGATATCGACCAGCTCGGCTATGAGCCCGAGCAGAAGCAGCTCCTGCTGGAC
GTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAGACGGTGTCGCTGTACACCTTCCT
GAACAAGCTGAACCAGGGCGACATCAACATTTCCACCGCGGAAGACCCGGCGGAAATCCAGCTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCGGGGCTGACCTTCGCCGCGGCGCTGAAGTCCTTTCTGCGGCAGGATCCCGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACCGCCGACATCTCCATCAAGGCCGCGCAAACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGACGCGCCGACCACGCTGACACGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCGCATCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCCTGCAAGAAGCCGGGTCCGCTGCCCAAGGAAACGCTG
CTGGACGCGGGCTTCAAGGAAGCAGACCTGGACGGCTCGTGGCAGCCGTACCATCCGGTCGGCTGCGAGGCATGCAACGG
CAGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCGCCCACG
GCACGGCGCTGCAGATCGCCGAACAGGCGCGCAAGGAAGGCGTGCTCTCGCTGCGCGATTCCGGCCTGCGGAAAGTGAAG
CAGGGGCTGACTTCCCTTGAGGAAGTGTTGGCCACGACCAACCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

52.93

94.957

0.503
  pilB Acinetobacter baumannii D1279779

49.738

99.652

0.496
  pilB Acinetobacter baylyi ADP1

49.554

97.565

0.483
  pilB Legionella pneumophila strain ERS1305867

47.163

98.087

0.463
  pilB Vibrio cholerae strain A1552

44.821

97.391

0.437
  pilB Vibrio parahaemolyticus RIMD 2210633

45.203

94.261

0.426
  pilB Vibrio campbellii strain DS40M4

44.526

95.304

0.424
  pilF Thermus thermophilus HB27

38.256

97.739

0.374