Detailed information NTG0533105

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLI11_RS09350
• Genome accession: NZ_CP069223
• Coordinates: 1855338..1855985 (-)
• Length: 215 a.a.
• NCBI ID: WP_004255240.1
• Uniprot ID: A0A1V0NHR8
• Organism: Lactococcus lactis subsp. lactis strain I.1.1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1850338..1860985

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLI11_RS09320 (LLI11_09275)	atpF	1850409..1850915 (-)	507	WP_058221761.1	F0F1 ATP synthase subunit B	-
LLI11_RS09325 (LLI11_09280)	atpB	1850930..1851643 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LLI11_RS09330 (LLI11_09285)	-	1851688..1851903 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LLI11_RS09335 (LLI11_09290)	-	1852087..1852863 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
LLI11_RS09340 (LLI11_09295)	comEC	1853148..1854707 (-)	1560	WP_405023164.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LLI11_RS13910 (LLI11_13895)	comEC	1854725..1855357 (-)	633	WP_284198769.1	ComEC/Rec2 family competence protein	Machinery gene
LLI11_RS09350 (LLI11_09300)	comEA	1855338..1855985 (-)	648	WP_004255240.1	ComEA family DNA-binding protein	Machinery gene
LLI11_RS09355 (LLI11_09305)	-	1856045..1857406 (-)	1362	WP_058221762.1	ABC transporter permease	-
LLI11_RS09360 (LLI11_09310)	-	1857403..1858335 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LLI11_RS09365 (LLI11_09315)	-	1858440..1858838 (-)	399	WP_004255236.1	hypothetical protein	-
LLI11_RS09370 (LLI11_09320)	-	1858942..1859505 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
LLI11_RS09375 (LLI11_09325)	-	1859678..1860856 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23651.83 Da
• Isoelectric Point: 5.0062

>NTDB_id=533105 LLI11_RS09350 WP_004255240.1 1855338..1855985(-) (comEA) [Lactococcus lactis subsp. lactis strain I.1.1]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSKTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=533105 LLI11_RS09350 WP_004255240.1 1855338..1855985(-) (comEA) [Lactococcus lactis subsp. lactis strain I.1.1]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAAAAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A1V0NHR8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386
comEA	Staphylococcus aureus MW2	37.089	99.07	0.367