Detailed information NTG0532745

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLNCDO700_RS09845
• Genome accession: NZ_CP069179
• Coordinates: 1944287..1944943 (-)
• Length: 218 a.a.
• NCBI ID: WP_014735120.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain NCDO700
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1939287..1949943

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLNCDO700_RS09820 (LLNCDO700_09800)	atpF	1939358..1939864 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
LLNCDO700_RS09825 (LLNCDO700_09805)	atpB	1939879..1940592 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LLNCDO700_RS09830 (LLNCDO700_09810)	-	1940639..1940854 (-)	216	WP_014570694.1	F0F1 ATP synthase subunit C	-
LLNCDO700_RS09835 (LLNCDO700_09815)	-	1941037..1941813 (-)	777	WP_011835694.1	alpha/beta hydrolase family protein	-
LLNCDO700_RS09840 (LLNCDO700_09820)	-	1942097..1944306 (-)	2210	Protein_1921	DNA internalization-related competence protein ComEC/Rec2	-
LLNCDO700_RS09845 (LLNCDO700_09825)	comEA	1944287..1944943 (-)	657	WP_014735120.1	helix-hairpin-helix domain-containing protein	Machinery gene
LLNCDO700_RS09850 (LLNCDO700_09830)	-	1945001..1946362 (-)	1362	WP_011835696.1	ABC transporter permease	-
LLNCDO700_RS09855 (LLNCDO700_09835)	-	1946359..1947291 (-)	933	WP_011835697.1	ABC transporter ATP-binding protein	-
LLNCDO700_RS09860 (LLNCDO700_09840)	-	1947397..1947795 (-)	399	WP_042747837.1	hypothetical protein	-
LLNCDO700_RS09865 (LLNCDO700_09845)	-	1947912..1948472 (-)	561	WP_011835699.1	GNAT family N-acetyltransferase	-
LLNCDO700_RS09870 (LLNCDO700_09850)	-	1948646..1949824 (-)	1179	WP_014735121.1	SLC13 family permease	-

Sequence

Protein

• Length: 218 a.a.
• Molecular weight: 23674.86 Da
• Isoelectric Point: 5.1730

>NTDB_id=532745 LLNCDO700_RS09845 WP_014735120.1 1944287..1944943(-) (comEA) [Lactococcus cremoris strain NCDO700]
MEKILDKLKEYWKIMLLVVCALIAGGILYIFTNSPKPAETLSIENLSSSSTKSSVSKFNSSSSEKNKNEIMVDLKGAVAK
PNVYQISSDERLVDLIRQAGGFTDQADPKSINLSAKLKDEEVIYVPKVGESSSSESTDSPTGSSVSNQVSTTSGPKVNIN
KADLTELQKLTGIGQKKAQDIIDFRMKNGDFKSIEDLGKVSGFGDKTLEKLKDEISID

Nucleotide

• Length: 657 bp

>NTDB_id=532745 LLNCDO700_RS09845 WP_014735120.1 1944287..1944943(-) (comEA) [Lactococcus cremoris strain NCDO700]
ATGGAAAAAATTTTAGATAAATTAAAAGAATACTGGAAAATAATGCTTTTAGTTGTTTGTGCACTTATTGCTGGTGGAAT
TCTTTATATTTTCACCAATTCACCAAAGCCAGCTGAAACTCTGTCAATAGAAAATTTGAGCTCTAGTTCTACCAAAAGCT
CTGTCAGTAAATTTAATAGTAGCAGTAGTGAAAAAAATAAAAATGAAATTATGGTTGATTTAAAAGGAGCGGTTGCAAAA
CCTAATGTATACCAAATTTCGTCAGATGAGCGCCTTGTTGATTTAATTAGGCAAGCGGGAGGATTTACTGACCAAGCAGA
CCCAAAATCAATCAATCTGTCAGCGAAACTTAAAGATGAAGAAGTGATTTATGTACCAAAAGTTGGGGAAAGTTCAAGTT
CAGAAAGTACTGACAGTCCTACTGGTAGCTCTGTCAGTAATCAAGTTTCAACGACAAGTGGTCCAAAAGTAAATATCAAT
AAAGCAGACCTGACAGAATTACAAAAACTGACTGGAATTGGTCAAAAAAAAGCGCAAGATATTATTGATTTTCGCATGAA
AAATGGTGACTTTAAATCAATAGAGGATTTGGGTAAAGTATCTGGCTTTGGGGATAAAACATTAGAAAAACTGAAAGATG
AGATTTCTATTGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (71-126)

• Helix-hairpin-helix motif (HHH_3) (154-215)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	98.165	100	0.982
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	41.41	100	0.431
comEA/celA/cilE	Streptococcus mitis SK321	41.778	100	0.431
comEA/celA/cilE	Streptococcus mitis NCTC 12261	40.529	100	0.422
comEA/celA/cilE	Streptococcus pneumoniae R6	40.991	100	0.417
comEA/celA/cilE	Streptococcus pneumoniae Rx1	40.991	100	0.417
comEA/celA/cilE	Streptococcus pneumoniae D39	40.991	100	0.417
comEA	Latilactobacillus sakei subsp. sakei 23K	37.021	100	0.399
comEA	Staphylococcus aureus MW2	35.931	100	0.381
comEA	Staphylococcus aureus N315	36.866	99.541	0.367