Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K9U12_RS11365 Genome accession   NZ_CP083481
Coordinates   2316567..2317952 (+) Length   461 a.a.
NCBI ID   WP_001025167.1    Uniprot ID   -
Organism   Escherichia coli strain elppa9     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2311567..2322952
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9U12_RS11340 (K9U12_11345) aroP 2312115..2313488 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  K9U12_RS11345 (K9U12_11350) ampE 2313531..2314385 (-) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  K9U12_RS11350 (K9U12_11355) ampD 2314382..2314933 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K9U12_RS11355 (K9U12_11360) nadC 2315021..2315914 (+) 894 WP_001135153.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K9U12_RS11360 (K9U12_11365) pilA 2316117..2316557 (+) 441 WP_020233150.1 prepilin peptidase-dependent pilin Machinery gene
  K9U12_RS11365 (K9U12_11370) pilB 2316567..2317952 (+) 1386 WP_001025167.1 type II secretion system protein GspE Machinery gene
  K9U12_RS11370 (K9U12_11375) hofC 2317942..2319144 (+) 1203 WP_021522995.1 protein transport protein HofC -
  K9U12_RS11375 (K9U12_11380) guaC 2319179..2320222 (-) 1044 WP_001217337.1 GMP reductase -
  K9U12_RS11380 (K9U12_11385) - 2320378..2320422 (-) 45 WP_120795372.1 protein YacM -
  K9U12_RS11385 (K9U12_11390) coaE 2320447..2321067 (+) 621 WP_228571342.1 dephospho-CoA kinase -
  K9U12_RS11390 (K9U12_11395) zapD 2321067..2321810 (+) 744 WP_001194736.1 cell division protein ZapD -
  K9U12_RS11395 (K9U12_11400) yacG 2321820..2322017 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  K9U12_RS11400 (K9U12_11405) mutT 2322219..2322617 (-) 399 WP_000736024.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50655.35 Da        Isoelectric Point: 6.6256

>NTDB_id=532404 K9U12_RS11365 WP_001025167.1 2316567..2317952(+) (pilB) [Escherichia coli strain elppa9]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGFYGRTALFE
VLPITPIIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=532404 K9U12_RS11365 WP_001025167.1 2316567..2317952(+) (pilB) [Escherichia coli strain elppa9]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTATTCATGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATACGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGGAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCCCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATTC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGACCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCGCATTGTCGCCGGCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCGCTGGCAAGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTACCCATAACACCGATCATACGCCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACACACGCCCGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.469

100

0.416
  pilB Legionella pneumophila strain ERS1305867

49.74

83.297

0.414
  pilB Haemophilus influenzae 86-028NP

39.915

100

0.408
  pilB Vibrio campbellii strain DS40M4

48.187

83.731

0.403
  pilB Vibrio parahaemolyticus RIMD 2210633

46.41

84.599

0.393
  pilB Vibrio cholerae strain A1552

46.41

84.599

0.393
  pilB Acinetobacter baylyi ADP1

45.22

83.948

0.38
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilB Haemophilus influenzae Rd KW20

43.928

83.948

0.369
  pilB Acinetobacter baumannii D1279779

43.864

83.08

0.364
  pilF Thermus thermophilus HB27

41.032

88.286

0.362