Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K9U12_RS11360 Genome accession   NZ_CP083481
Coordinates   2316117..2316557 (+) Length   146 a.a.
NCBI ID   WP_020233150.1    Uniprot ID   -
Organism   Escherichia coli strain elppa9     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2311117..2321557
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9U12_RS11340 (K9U12_11345) aroP 2312115..2313488 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  K9U12_RS11345 (K9U12_11350) ampE 2313531..2314385 (-) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  K9U12_RS11350 (K9U12_11355) ampD 2314382..2314933 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K9U12_RS11355 (K9U12_11360) nadC 2315021..2315914 (+) 894 WP_001135153.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K9U12_RS11360 (K9U12_11365) pilA 2316117..2316557 (+) 441 WP_020233150.1 prepilin peptidase-dependent pilin Machinery gene
  K9U12_RS11365 (K9U12_11370) pilB 2316567..2317952 (+) 1386 WP_001025167.1 type II secretion system protein GspE Machinery gene
  K9U12_RS11370 (K9U12_11375) hofC 2317942..2319144 (+) 1203 WP_021522995.1 protein transport protein HofC -
  K9U12_RS11375 (K9U12_11380) guaC 2319179..2320222 (-) 1044 WP_001217337.1 GMP reductase -
  K9U12_RS11380 (K9U12_11385) - 2320378..2320422 (-) 45 WP_120795372.1 protein YacM -
  K9U12_RS11385 (K9U12_11390) coaE 2320447..2321067 (+) 621 WP_228571342.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=532403 K9U12_RS11360 WP_020233150.1 2316117..2316557(+) (pilA) [Escherichia coli strain elppa9]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTNMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=532403 K9U12_RS11360 WP_020233150.1 2316117..2316557(+) (pilA) [Escherichia coli strain elppa9]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCAACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGTAATGGCATCCCCTCGCCTACCACTACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGTTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTTCGCTTTGATGACGCAAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363