Detailed information NTG0530536

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: JM964_RS00735
• Genome accession: NZ_CP068708
• Coordinates: 119007..119441 (+)
• Length: 144 a.a.
• NCBI ID: WP_024395445.1
• Uniprot ID: -
• Organism: Streptococcus suis strain ID33329
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 114007..124441

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JM964_RS00700 (JM964_00700)	-	114749..115114 (+)	366	WP_024395451.1	DUF1033 family protein	-
JM964_RS00705 (JM964_00705)	-	115149..116099 (-)	951	WP_014637311.1	S66 peptidase family protein	-
JM964_RS00710 (JM964_00710)	comYA	116185..117135 (+)	951	WP_105140681.1	competence type IV pilus ATPase ComGA	Machinery gene
JM964_RS00715 (JM964_00715)	comYB	117047..118084 (+)	1038	WP_238492613.1	competence type IV pilus assembly protein ComGB	Machinery gene
JM964_RS00720 (JM964_00720)	comYC	118086..118367 (+)	282	WP_024395447.1	competence type IV pilus major pilin ComGC	Machinery gene
JM964_RS00725 (JM964_00725)	comGD	118348..118755 (+)	408	WP_024395446.1	competence type IV pilus minor pilin ComGD	-
JM964_RS00730 (JM964_00730)	comYE	118727..119020 (+)	294	WP_002936548.1	competence type IV pilus minor pilin ComGE	Machinery gene
JM964_RS00735 (JM964_00735)	comGF/cglF	119007..119441 (+)	435	WP_024395445.1	competence type IV pilus minor pilin ComGF	Machinery gene
JM964_RS00740 (JM964_00740)	comGG	119419..119829 (+)	411	WP_024395444.1	competence type IV pilus minor pilin ComGG	-
JM964_RS00745 (JM964_00745)	comYH	119886..120839 (+)	954	WP_044776365.1	class I SAM-dependent methyltransferase	Machinery gene
JM964_RS00750 (JM964_00750)	-	120889..122076 (+)	1188	WP_014637319.1	acetate kinase	-
JM964_RS00755 (JM964_00755)	-	122337..122888 (+)	552	WP_014637320.1	folate family ECF transporter S component	-
JM964_RS00760 (JM964_00760)	-	122944..124200 (+)	1257	WP_024401511.1	bifunctional folylpolyglutamate synthase/dihydrofolate synthase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16433.77 Da
• Isoelectric Point: 6.9832

>NTDB_id=530536 JM964_RS00735 WP_024395445.1 119007..119441(+) (comGF/cglF) [Streptococcus suis strain ID33329]
MLKSKIPAFTLLECLVALIILSGSLLVFEGLSKLLVQEAHYQGQTVQKEWLVFSSQLRSEWDQAELVKVENGKVYVNKGE
QALAFGKSRSDDFRKTNDRGQGYQPMLYQVDSAAISQENQLVRIDFSFKNGEERTFIYAFKEKS

Nucleotide

• Length: 435 bp

>NTDB_id=530536 JM964_RS00735 WP_024395445.1 119007..119441(+) (comGF/cglF) [Streptococcus suis strain ID33329]
TTGTTAAAAAGTAAAATTCCTGCTTTTACTCTCTTGGAATGCTTGGTAGCTTTAATCATCCTATCAGGAAGTCTTCTGGT
CTTTGAAGGCTTGTCAAAACTGCTGGTCCAAGAAGCGCATTACCAAGGTCAGACCGTTCAAAAGGAATGGTTGGTCTTCT
CTAGTCAATTGCGGTCGGAGTGGGACCAGGCTGAATTGGTCAAGGTTGAAAATGGCAAGGTCTATGTCAACAAAGGTGAA
CAGGCTTTGGCCTTTGGCAAGTCACGGTCGGATGATTTTCGGAAAACAAATGACAGGGGACAGGGTTACCAGCCCATGCT
CTATCAGGTGGATAGCGCAGCCATTTCCCAAGAAAACCAGCTGGTGCGCATCGACTTTAGCTTTAAAAACGGAGAGGAGC
GGACCTTTATCTATGCTTTTAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (37-134)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae TIGR4	53.901	97.917	0.528
comGF/cglF	Streptococcus pneumoniae Rx1	53.901	97.917	0.528
comGF/cglF	Streptococcus pneumoniae D39	53.901	97.917	0.528
comGF/cglF	Streptococcus pneumoniae R6	53.901	97.917	0.528
comGF/cglF	Streptococcus mitis NCTC 12261	54.478	93.056	0.507
comYF	Streptococcus mutans UA140	50	100	0.5
comYF	Streptococcus mutans UA159	49.306	100	0.493
comGF/cglF	Streptococcus mitis SK321	51.493	93.056	0.479
comGF	Lactococcus lactis subsp. cremoris KW2	46.809	97.917	0.458