Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JHS79_RS14430 Genome accession   NZ_CP068641
Coordinates   2902946..2904631 (+) Length   561 a.a.
NCBI ID   WP_140126226.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain VP157     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2897946..2909631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHS79_RS14410 (JHS79_14245) pdhR 2899552..2900319 (-) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  JHS79_RS14415 (JHS79_14250) ampD 2900725..2901276 (-) 552 WP_140126227.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  JHS79_RS14420 (JHS79_14255) nadC 2901369..2902256 (+) 888 WP_015297249.1 carboxylating nicotinate-nucleotide diphosphorylase -
  JHS79_RS14425 (JHS79_14260) pilA 2902521..2902940 (+) 420 WP_025442238.1 pilin Machinery gene
  JHS79_RS14430 (JHS79_14265) pilB 2902946..2904631 (+) 1686 WP_140126226.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JHS79_RS14435 (JHS79_14270) pilC 2904655..2905878 (+) 1224 WP_005479682.1 type II secretion system F family protein Machinery gene
  JHS79_RS14440 (JHS79_14275) pilD 2905943..2906812 (+) 870 WP_005493974.1 prepilin peptidase Machinery gene
  JHS79_RS14445 (JHS79_14280) coaE 2906813..2907427 (+) 615 WP_025633754.1 dephospho-CoA kinase -
  JHS79_RS14450 (JHS79_14285) zapD 2907455..2908195 (+) 741 WP_015297254.1 cell division protein ZapD -
  JHS79_RS14455 (JHS79_14290) yacG 2908264..2908458 (+) 195 WP_005462546.1 DNA gyrase inhibitor YacG -
  JHS79_RS14460 (JHS79_14295) rplS 2908840..2909193 (-) 354 WP_005462554.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62382.27 Da        Isoelectric Point: 5.4089

>NTDB_id=530165 JHS79_RS14430 WP_140126226.1 2902946..2904631(+) (pilB) [Vibrio parahaemolyticus strain VP157]
MLTNLSTVLRQKGLLTFSQEESLIEQVKASGISMPEALLSSGFFTSSELAEHLSSIFGLSQPELSQYEYASLCQQLGLRE
LITRHNALPLHRTPSTLLLAVADPTNQQAEDDFRFATGLQVELVLVDFRELSAAIRRLYGRAIGQERSQLKEINQEELAS
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPNHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLEALRRPQGMI
LMTGPTGSGKTVSLYTGLNILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETIIRLSNMGVESFNLASSLSLIIAQRLARKLCPYCKQPQEHTVQLQHLGIQTTDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDESLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=530165 JHS79_RS14430 WP_140126226.1 2902946..2904631(+) (pilB) [Vibrio parahaemolyticus strain VP157]
ATGTTAACAAACCTCTCAACGGTTCTTCGTCAAAAAGGATTATTGACGTTTTCCCAAGAAGAATCGTTAATAGAGCAAGT
TAAAGCTTCTGGCATCTCGATGCCGGAAGCTTTACTCAGTTCTGGATTCTTCACATCAAGCGAACTGGCCGAGCACTTAA
GTTCTATTTTTGGCTTAAGTCAGCCCGAGTTATCTCAATATGAATATGCTTCTCTTTGCCAACAGCTCGGCCTACGTGAA
TTAATCACACGACATAATGCACTCCCACTCCACCGTACTCCTTCAACTTTATTATTAGCGGTTGCCGACCCCACGAATCA
ACAAGCTGAAGATGATTTCCGCTTTGCCACTGGTTTGCAGGTCGAACTGGTTCTGGTCGATTTTCGCGAACTAAGTGCCG
CAATTCGTCGCTTGTACGGGCGTGCCATTGGTCAGGAACGCTCCCAGCTCAAAGAGATCAACCAAGAAGAGCTAGCCAGC
TTAGTGGATGTCGGTGCAGATGAAATCGACAATATCGAAGACTTGAGCCAAGACGAATCTCCTGTTAGCCGTTACATCAA
CCAAATTCTGTTGGACGCTGTACGCAAAGGCGCATCCGATATCCACTTTGAACCTTATGAAAAGATGTACCGAGTTCGTC
TACGTTGCGATGGCATTTTGATCGAAACTCAGCAACCGCCAAATCACTTGAGTCGTCGCTTATCAGCTCGTATAAAAATT
CTCTCTAAACTTGATATTGCCGAGCGACGTTTACCACAAGATGGGCGAATTAAACTCAAACTGAATCAAGACACCGCGAT
TGATATGCGGGTATCCACTCTACCTACCTTGTTTGGAGAGAAGATCGTACTACGACTGCTCGATAGCAGCTCCGCATCAC
TGGATATCGACAAGCTTGGTTATAGTGAGCAACAAAAACAGCTGTATTTAGAGGCTCTGCGTCGTCCACAAGGTATGATT
CTGATGACAGGTCCAACAGGAAGCGGCAAAACCGTTTCGTTGTACACTGGGCTAAATATTCTCAATAAACCAGAGATCAA
CATCTCCACAGCGGAAGACCCAGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTCCAACCTAAGATTGGTTTTG
GCTTTGCAGAAGCACTACGTTCTTTTCTGCGTCAAGATCCCGATGTGGTCATGGTGGGTGAAATTCGAGACCTAGATACT
GCGGAAATCGCGATTAAGGCGTCCCAAACCGGTCACTTAGTGCTCTCTACTTTGCATACCAACTCTGCAGCTGAAACCAT
CATTCGCTTATCAAACATGGGCGTGGAAAGTTTTAACCTCGCCTCTTCACTTAGCTTGATTATTGCCCAACGCCTAGCTC
GCAAACTTTGTCCATATTGTAAACAACCGCAAGAGCATACCGTTCAACTTCAGCACCTCGGCATCCAAACAACTGACAAC
ATCTTTAAAGCCAATCCAGATGGTTGCAACGAATGTACCCATGGTTATTCTGGCCGAACGGGTATCTATGAAGTCATGCG
CTTTGATGAATCTCTATCCGAAGCTCTTATTAAAGGAGCCTCGGTACATGAACTGGAAAAGCTTGCCATTGCGAACGGCA
TGAGCACCTTGCAAATGTCCGGAATAGAAAAACTCAAACAAGGCATCACAAGCTTTAGTGAGTTACAACGCGTGCTCTAT
TTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

97.861

100

0.979
  pilB Vibrio campbellii strain DS40M4

90.553

100

0.906
  pilB Vibrio cholerae strain A1552

74.021

100

0.742
  pilB Acinetobacter baumannii D1279779

51.504

94.831

0.488
  pilB Acinetobacter baylyi ADP1

48.294

99.287

0.479
  pilB Legionella pneumophila strain ERS1305867

49.351

96.078

0.474
  pilF Neisseria gonorrhoeae MS11

44.858

100

0.451
  pilB/pilB1 Synechocystis sp. PCC 6803

37.669

100

0.398
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.332

100

0.367
  pilF Thermus thermophilus HB27

36.525

100

0.367