Detailed information NTG0530167

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: JHS79_RS14440
• Genome accession: NZ_CP068641
• Coordinates: 2905943..2906812 (+)
• Length: 289 a.a.
• NCBI ID: WP_005493974.1
• Uniprot ID: A0A249W3M2
• Organism: Vibrio parahaemolyticus strain VP157
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2900943..2911812

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JHS79_RS14420 (JHS79_14255)	nadC	2901369..2902256 (+)	888	WP_015297249.1	carboxylating nicotinate-nucleotide diphosphorylase	-
JHS79_RS14425 (JHS79_14260)	pilA	2902521..2902940 (+)	420	WP_025442238.1	pilin	Machinery gene
JHS79_RS14430 (JHS79_14265)	pilB	2902946..2904631 (+)	1686	WP_140126226.1	type IV-A pilus assembly ATPase PilB	Machinery gene
JHS79_RS14435 (JHS79_14270)	pilC	2904655..2905878 (+)	1224	WP_005479682.1	type II secretion system F family protein	Machinery gene
JHS79_RS14440 (JHS79_14275)	pilD	2905943..2906812 (+)	870	WP_005493974.1	prepilin peptidase	Machinery gene
JHS79_RS14445 (JHS79_14280)	coaE	2906813..2907427 (+)	615	WP_025633754.1	dephospho-CoA kinase	-
JHS79_RS14450 (JHS79_14285)	zapD	2907455..2908195 (+)	741	WP_015297254.1	cell division protein ZapD	-
JHS79_RS14455 (JHS79_14290)	yacG	2908264..2908458 (+)	195	WP_005462546.1	DNA gyrase inhibitor YacG	-
JHS79_RS14460 (JHS79_14295)	rplS	2908840..2909193 (-)	354	WP_005462554.1	50S ribosomal protein L19	-
JHS79_RS14465 (JHS79_14300)	trmD	2909235..2909978 (-)	744	WP_005462562.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
JHS79_RS14470 (JHS79_14305)	rimM	2910006..2910554 (-)	549	WP_005462552.1	ribosome maturation factor RimM	-
JHS79_RS14475 (JHS79_14310)	rpsP	2910583..2910831 (-)	249	WP_005379962.1	30S ribosomal protein S16	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32377.43 Da
• Isoelectric Point: 7.1971

>NTDB_id=530167 JHS79_RS14440 WP_005493974.1 2905943..2906812(+) (pilD) [Vibrio parahaemolyticus strain VP157]
MEVFQYYPWLFVVFASIFGLIVGSFLNVVIYRLPKIMELEWRRECAESFPEYKIKPPQEVLTLSVPRSSCQNCATPIRIR
DNIPVISWLLLKGKCHHCHTAISPRYPLIELLTAACAGFVAYHFGFSYFTVALIFFTFVLIAATFIDLDTMLLPDQLTLP
LTWAGIALALTEISPVSLQDAVIGAIAGYLCLWSVYWGFKLLTGKEGMGYGDFKLLAALGAWLGWQSLPMIILLSSVVGV
IFGLVQLRLQKQGIERAFPFGPYLAIAGWASLIWGDQILSWYFTSILGV

Nucleotide

• Length: 870 bp

>NTDB_id=530167 JHS79_RS14440 WP_005493974.1 2905943..2906812(+) (pilD) [Vibrio parahaemolyticus strain VP157]
ATGGAAGTGTTCCAGTACTACCCTTGGCTATTCGTGGTGTTTGCCAGTATCTTCGGTTTAATTGTGGGTAGTTTTCTTAA
TGTAGTCATTTATCGGCTACCTAAAATCATGGAATTAGAATGGCGCCGTGAATGCGCCGAATCATTCCCTGAATATAAGA
TTAAGCCACCTCAAGAAGTTTTGACATTAAGCGTGCCTCGCTCTTCGTGCCAAAACTGCGCGACGCCAATTCGTATTCGA
GACAATATTCCTGTAATCAGCTGGCTACTGTTAAAAGGAAAATGCCATCATTGTCATACCGCAATTAGTCCTCGATATCC
GCTGATAGAATTACTCACTGCAGCGTGCGCTGGTTTTGTGGCTTATCATTTCGGCTTTAGCTACTTTACGGTTGCGTTAA
TCTTTTTCACATTTGTCTTAATAGCCGCGACTTTTATCGATCTCGATACTATGCTGCTACCGGACCAATTAACATTACCA
TTAACTTGGGCTGGCATTGCGTTAGCATTAACAGAAATAAGCCCTGTTAGTCTACAAGACGCCGTAATTGGTGCGATTGC
CGGTTATTTGTGTCTTTGGTCAGTATATTGGGGTTTCAAACTGCTTACTGGTAAAGAAGGCATGGGTTACGGTGATTTTA
AATTGCTGGCTGCCCTTGGCGCTTGGCTAGGATGGCAATCATTACCAATGATCATCCTACTTTCTTCCGTCGTCGGTGTG
ATATTCGGGCTCGTTCAGTTACGACTACAAAAACAAGGTATAGAGAGAGCTTTCCCTTTCGGCCCATATTTAGCGATTGC
AGGATGGGCAAGCTTAATCTGGGGCGATCAAATTCTAAGTTGGTACTTCACGTCAATTTTAGGGGTGTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

3D structure

• AlphaFold DB: A0A249W3M2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	88.927	100	0.889
pilD	Vibrio cholerae strain A1552	73.611	99.654	0.734
pilD	Acinetobacter baumannii D1279779	52.239	92.734	0.484
pilD	Acinetobacter nosocomialis M2	51.866	92.734	0.481
pilD	Neisseria gonorrhoeae MS11	47.101	95.502	0.45