Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABCA12_RS16010 Genome accession   NZ_CP068253
Coordinates   3355397..3356572 (+) Length   391 a.a.
NCBI ID   WP_004914541.1    Uniprot ID   S7Y0P3
Organism   Acinetobacter junii strain CAM121     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3350397..3361572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABCA12_RS15985 (ABCA12_3258) - 3351061..3351534 (-) 474 WP_042891865.1 hypothetical protein -
  ABCA12_RS15990 (ABCA12_3259) guaD 3351720..3353045 (+) 1326 WP_201897937.1 guanine deaminase -
  ABCA12_RS15995 (ABCA12_3261) hpt 3353262..3353789 (+) 528 WP_004914531.1 hypoxanthine phosphoribosyltransferase -
  ABCA12_RS16000 (ABCA12_3262) - 3354018..3354269 (+) 252 WP_004949988.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  ABCA12_RS16005 (ABCA12_3263) - 3354319..3355016 (-) 698 Protein_3129 DsbC family protein -
  ABCA12_RS16010 (ABCA12_3264) pilU 3355397..3356572 (+) 1176 WP_004914541.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABCA12_RS16015 (ABCA12_3265) mnmE 3356628..3357983 (-) 1356 WP_026057420.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  ABCA12_RS16020 (ABCA12_3266) yidC 3358074..3359831 (-) 1758 WP_201897938.1 membrane protein insertase YidC -
  ABCA12_RS16025 (ABCA12_3267) yidD 3359837..3360157 (-) 321 WP_004950001.1 membrane protein insertion efficiency factor YidD -
  ABCA12_RS16030 (ABCA12_3268) rnpA 3360167..3360562 (-) 396 WP_004950005.1 ribonuclease P protein component -
  ABCA12_RS16035 (ABCA12_3269) rpmH 3360591..3360725 (-) 135 WP_000831329.1 50S ribosomal protein L34 -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 44323.84 Da        Isoelectric Point: 6.3220

>NTDB_id=528652 ABCA12_RS16010 WP_004914541.1 3355397..3356572(+) (pilU) [Acinetobacter junii strain CAM121]
MYTTELLEEAKTFMFHMLTKVVEYGGSDLFITADFPPSIKLQGLMRPLGQQALTAEKTKLFAYSLMNDKQRLDFENDWEC
NFAINVPNVSRFRVNVFKQQLQIGMVIRTITSEIPTFQKLKLPESLKNVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHTWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPEERRNQLLMDLSSNMKAIISQRLVRTQDGQGRRAAIEILLNTPLIADNILKGQFHVLKEIMTKS
RELGMQTFDQALFDLYNEGAISYDEALRNADSVNELRLQIKLKSSRQDGMPVSTTSLNMVKEEEVEEQEEA

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=528652 ABCA12_RS16010 WP_004914541.1 3355397..3356572(+) (pilU) [Acinetobacter junii strain CAM121]
ATGTATACCACTGAATTATTAGAAGAAGCCAAAACCTTTATGTTTCATATGCTCACTAAAGTAGTTGAGTACGGGGGATC
TGATTTATTTATTACCGCAGATTTCCCACCTAGCATTAAGCTACAGGGTTTGATGCGCCCTTTAGGACAACAAGCGCTTA
CCGCAGAAAAAACCAAGTTGTTTGCTTATAGTTTAATGAATGACAAACAACGTCTAGACTTCGAAAATGATTGGGAATGT
AACTTTGCCATTAATGTCCCGAATGTTTCAAGGTTTCGGGTCAATGTATTCAAACAACAATTACAAATAGGCATGGTGAT
TCGAACCATTACGTCTGAAATTCCAACCTTTCAAAAACTAAAACTCCCAGAATCACTAAAAAATGTGATTATGGAAAAAC
GAGGTTTGGTACTGGTGGTTGGCGGAACAGGATCAGGAAAATCAACCTCTTTAGCAGCCATGATTGATCATCGAAATGAG
AACTCTGCTGGACATATCATTACCGTTGAAGATCCTGTTGAATACGTCCATAAACACAAAAAATCCATGATCACGCACCG
TGAGGTTGGAGTTGATTGTCATACATGGCATCACGCGTTGAAAAATACGCTACGCCAAGCACCAGATGTGATTTTAATTG
GTGAAATACGTGATACTGAAACGATGGAACATGCGATTGCTTTTGCTGAAACAGGGCATCTATGTTTGGGAACACTGCAC
GCCAACAATGCCAATCAGGCACTGGATCGTATTATTAACTTTTTCCCAGAGGAACGTCGCAATCAACTGTTGATGGATCT
CTCATCCAATATGAAAGCGATTATTTCTCAACGTCTCGTGCGAACTCAAGATGGACAAGGTCGTCGAGCTGCGATTGAAA
TCCTCCTCAATACACCATTAATCGCAGACAATATTTTGAAAGGGCAATTCCATGTGCTCAAAGAGATCATGACCAAATCT
CGCGAGTTAGGTATGCAGACCTTTGATCAGGCTTTGTTTGATCTATATAACGAAGGTGCAATTAGTTACGATGAAGCATT
GCGTAACGCCGACTCTGTCAATGAGTTACGCTTGCAAATTAAATTAAAAAGTTCACGCCAAGATGGGATGCCCGTTTCAA
CCACTTCACTGAACATGGTCAAAGAAGAGGAAGTTGAGGAGCAAGAAGAAGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S7Y0P3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.197

93.606

0.545
  pilU Acinetobacter baylyi ADP1

55.62

88.747

0.494
  pilU Vibrio cholerae strain A1552

52.601

88.491

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.284

85.678

0.371
  pilT Acinetobacter nosocomialis M2

44.172

83.376

0.368
  pilT Acinetobacter baumannii D1279779

44.172

83.376

0.368
  pilT Acinetobacter baumannii strain A118

44.172

83.376

0.368
  pilT Acinetobacter baylyi ADP1

42.56

85.934

0.366