Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6I50_RS07645 Genome accession   NZ_CP068195
Coordinates   1566978..1568690 (-) Length   570 a.a.
NCBI ID   WP_087011197.1    Uniprot ID   A0A1R7Q9V2
Organism   Acinetobacter johnsonii strain FDAARGOS_1093     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1561978..1573690
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6I50_RS07615 (I6I50_07615) - 1562076..1562399 (+) 324 WP_004692718.1 pyrimidine/purine nucleoside phosphorylase -
  I6I50_RS07620 (I6I50_07620) rlmB 1562542..1563291 (+) 750 WP_004692717.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  I6I50_RS07625 (I6I50_07625) - 1563324..1564241 (+) 918 WP_201707084.1 DMT family transporter -
  I6I50_RS07630 (I6I50_07630) coaE 1564255..1564854 (-) 600 WP_201707085.1 dephospho-CoA kinase -
  I6I50_RS07635 (I6I50_07635) pilD 1564861..1565721 (-) 861 WP_201707086.1 A24 family peptidase Machinery gene
  I6I50_RS07640 (I6I50_07640) pilC 1565721..1566947 (-) 1227 WP_004692713.1 type II secretion system F family protein Machinery gene
  I6I50_RS07645 (I6I50_07645) pilB 1566978..1568690 (-) 1713 WP_087011197.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6I50_RS07650 (I6I50_07650) tpiA 1568970..1569761 (+) 792 WP_195728536.1 triose-phosphate isomerase -
  I6I50_RS07655 (I6I50_07655) secG 1569775..1570104 (+) 330 WP_004692710.1 preprotein translocase subunit SecG -
  I6I50_RS07685 (I6I50_07685) rimP 1571058..1571582 (+) 525 WP_004692708.1 ribosome maturation factor RimP -
  I6I50_RS07690 (I6I50_07690) nusA 1571622..1573106 (+) 1485 WP_004692706.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63593.92 Da        Isoelectric Point: 5.0082

>NTDB_id=527945 I6I50_RS07645 WP_087011197.1 1566978..1568690(-) (pilB) [Acinetobacter johnsonii strain FDAARGOS_1093]
MSVSNGLPKFTGFIRRLVEDGRMTAENMQQAMLSAKKAQQDIVPYLIENHKLSPQMIAETISLEFGEPVFDLSVYDSVQI
LRDLVEDKLITKHRVLPIYRRGNILYIATSNPTNMDAMDAIRFNSKMNIEAIIVEHPQLEKLIEQNFAQADSFEFSDEDI
DLDLEQDHQNSNDEEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKDLFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAAVDIPEQSLLEMGF
TSTDIKDPDFKVYQPVGCAECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=527945 I6I50_RS07645 WP_087011197.1 1566978..1568690(-) (pilB) [Acinetobacter johnsonii strain FDAARGOS_1093]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGACGCATGACTGCTGAAAA
TATGCAGCAGGCAATGCTCTCTGCAAAAAAGGCTCAGCAAGACATCGTTCCTTATCTGATTGAAAATCATAAACTCTCCC
CACAAATGATCGCTGAAACGATTTCTTTAGAGTTTGGCGAGCCTGTTTTTGACTTAAGTGTCTATGATTCGGTACAAATT
CTACGGGATCTGGTCGAAGACAAACTTATCACCAAGCATCGTGTATTGCCGATTTACCGTCGTGGCAATATTCTCTATAT
CGCCACCAGCAATCCAACCAACATGGATGCGATGGACGCGATTCGTTTCAATTCTAAAATGAACATTGAAGCGATTATTG
TTGAACATCCTCAGCTTGAAAAACTAATCGAACAAAATTTCGCGCAAGCTGATAGCTTTGAATTTTCTGATGAAGATATT
GATTTAGACCTAGAACAAGACCATCAGAACTCAAATGACGAAGAAGATGACTCACCTCAAGGCGATGAAGCACCGATTGT
CAAATACATCAATAAATTACTGATCGATGCGATTCGTATGGGCGCATCAGATTTACACTTTGAACCTTATGAAAAATCTT
ACCGTGTTCGTTATCGCGTAGATGGAGTTCTGCGTCATATTGCCAGTCCGCCACTACAACTGGCGACTCGTCTTGCCTCA
CGTTTAAAAGTTATGTCACAAATGGACATCTCTGAAAAGCGCGTTCCACAAGATGGCCGAATTAAATTAAAACTGTCAAA
AAGTAAAGCCATCGACTTTCGTGTCAACTCACTTCCAACCTTGTTTGGTGAGAAATTGGTTCTGCGTATTCTCGATCCAT
CCAGTGCTATGCTGGGGATTGACGCTTTAGGTTATGAACCAGATCAAAAAGACCTATTTATGCAGGCTTTAGACAAACCA
CAAGGCATGTTGTTAATTACTGGCCCGACGGGTTCAGGTAAAACGGTATCGCTATATACAGGTTTGAATATTCTAAATAA
AGAAGACACCAATATTTCAACTGCGGAAGACCCTGTTGAGATTAACTTAGAAGGCATTAATCAGGTCAATGTGAACAATA
AAGTCGGCCTCACCTTCTCTGCTGCACTGAAGTCCTTCTTACGTCAGGATCCTGATATTGTGATGGTGGGGGAGATTCGT
GACCTTGAAACAGCCGAAATCGCGATTAAAGCCGCACAGACAGGTCACATGGTCATGTCGACACTCCACACCAATAGTGC
ACCTGAAACCCTGACACGCTTACGTAATATGGGGGTTCCCTCTTTTAACATTGCGACATCGGTCAATTTGGTGATTGCAC
AGCGTTTGGCACGCCGCTTATGCAGCCAATGCAAAGCCGCTGTCGATATTCCTGAACAAAGTTTGCTCGAAATGGGCTTT
ACTTCCACAGATATCAAAGATCCAGACTTCAAAGTTTATCAACCGGTGGGGTGTGCTGAGTGCCGTGAAGGTTATAAAGG
ACGTGTTGGTATTTATGAAGTCATGAAAGTCACGCCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCTTACAGA
TTGCGGAAGCATCTGCAAAATTAGGATTTAACAATTTACGTCGTTCAGGTTTACTCAAGGTTATGCAGGGGGTCACTTCC
TTACAAGAAGTGAATCGTGTAACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R7Q9V2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.228

100

0.812
  pilB Acinetobacter baylyi ADP1

76.667

100

0.767
  pilB Legionella pneumophila strain ERS1305867

53.933

93.684

0.505
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio cholerae strain A1552

48.677

99.474

0.484
  pilB Vibrio campbellii strain DS40M4

47.849

97.895

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

50.677

90.702

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

34.41

100

0.374
  pilF Thermus thermophilus HB27

36.602

100

0.367