Detailed information NTG0527932

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: I6I50_RS04035
• Genome accession: NZ_CP068195
• Coordinates: 798756..799814 (-)
• Length: 352 a.a.
• NCBI ID: WP_004978085.1
• Uniprot ID: N9D0Y2
• Organism: Acinetobacter johnsonii strain FDAARGOS_1093
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 793756..804814

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6I50_RS04010 (I6I50_04010)	-	794185..794667 (-)	483	WP_249303512.1	shikimate kinase	-
I6I50_RS04015 (I6I50_04015)	pilQ	794756..796834 (-)	2079	WP_159151545.1	type IV pilus secretin PilQ	Machinery gene
I6I50_RS04020 (I6I50_04020)	pilP	796854..797381 (-)	528	WP_094148569.1	pilus assembly protein PilP	Machinery gene
I6I50_RS04025 (I6I50_04025)	pilO	797381..798103 (-)	723	WP_004691497.1	type 4a pilus biogenesis protein PilO	Machinery gene
I6I50_RS04030 (I6I50_04030)	pilN	798100..798756 (-)	657	WP_004691498.1	PilN domain-containing protein	Machinery gene
I6I50_RS04035 (I6I50_04035)	pilM	798756..799814 (-)	1059	WP_004978085.1	pilus assembly protein PilM	Machinery gene
I6I50_RS04040 (I6I50_04040)	ponA	799980..802508 (+)	2529	WP_201706860.1	penicillin-binding protein PBP1a	-
I6I50_RS04045 (I6I50_04045)	-	802540..803346 (-)	807	WP_201706861.1	putative RNA methyltransferase	-
I6I50_RS04050 (I6I50_04050)	mraY	803416..804534 (-)	1119	WP_201706862.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38937.50 Da
• Isoelectric Point: 4.4181

>NTDB_id=527932 I6I50_RS04035 WP_004978085.1 798756..799814(-) (pilM) [Acinetobacter johnsonii strain FDAARGOS_1093]
MLRLYRKPSKGLIGVDISSTSVKVLELSVKNNRYWVESYALVPLPEGSVVEKNILNPEAVGDALERAVNLANVQSTDVAL
AVPTSMVITKIIEMDADMNDDEREIQIRDDAEQYIPFPLDEASLDFEVLPDRLANPNRVNVLLVATRIENVEARAEALEL
GGLTPKIADVESFAIENAFKVFSDTLPMGVNTVGILDIGHTMTTLSVMQNNKVIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKSRTLPDDYDIEVLEPFLEAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDIKKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=527932 I6I50_RS04035 WP_004978085.1 798756..799814(-) (pilM) [Acinetobacter johnsonii strain FDAARGOS_1093]
GTGCTCAGGTTATATCGTAAGCCAAGTAAGGGGTTAATAGGAGTAGATATTAGTTCGACTTCTGTTAAGGTATTGGAACT
TTCTGTTAAAAACAATCGGTATTGGGTGGAGAGCTATGCACTTGTCCCTTTACCAGAAGGAAGTGTGGTCGAAAAAAATA
TATTGAATCCAGAAGCAGTTGGTGATGCCCTTGAGCGTGCGGTAAATTTGGCCAATGTTCAATCGACAGACGTAGCACTG
GCAGTTCCGACTTCCATGGTGATTACTAAAATCATTGAAATGGATGCCGATATGAACGATGACGAACGTGAAATTCAGAT
TCGTGATGATGCTGAGCAATATATTCCATTCCCATTAGATGAAGCGAGTTTAGACTTTGAAGTCCTTCCAGACCGTTTAG
CCAATCCTAATCGTGTCAATGTACTCCTCGTTGCAACCCGCATTGAAAATGTAGAGGCACGCGCCGAAGCATTGGAGTTG
GGCGGTTTAACGCCTAAGATTGCCGATGTTGAAAGTTTCGCCATTGAAAATGCATTCAAAGTTTTTTCAGATACGCTTCC
AATGGGCGTAAATACTGTTGGGATTCTGGACATCGGTCACACCATGACCACCTTGTCCGTCATGCAAAATAATAAAGTCA
TTTATACCCGTGAACAGGTGTTCGGGGGTAAACAACTGACGCAAGAAATTCAAAATCGTTATGGTTTGTCTTTTGAAGAA
GCTGGACGTGCCAAAAAAAGCCGTACCCTGCCTGACGACTATGATATTGAAGTCCTTGAACCTTTTCTTGAAGCTGTGGT
GCAACAAGCAGCACGCTCTTTGCAGTTCTTCTTTTCGTCCTCTCAATTTAATGAGATCGACCATATTTTATTGGCAGGGG
GGAATGCCAATATTCCAGGACTGGCGAAGTTATTACAACAAAAATTAGGTTACCGGGTCACCATTGCCAACCCATTTTTA
CAGATGGGCTTTTCTCCACAAATCGACATTAAAAAAATTGAAAATGATGCTTCATCACTCATGGTGGCATGTGGATTGGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9D0Y2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	85.227	100	0.852
comM	Acinetobacter nosocomialis M2	85.227	100	0.852
comM	Acinetobacter baylyi ADP1	80.398	100	0.804
pilM	Legionella pneumophila strain ERS1305867	40.96	100	0.412