Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6I51_RS13595 Genome accession   NZ_CP068187
Coordinates   2679055..2680773 (-) Length   572 a.a.
NCBI ID   WP_201702307.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain FDAARGOS_1094     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2674055..2685773
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6I51_RS13565 (I6I51_13570) - 2674154..2674477 (+) 324 WP_125273379.1 pyrimidine/purine nucleoside phosphorylase -
  I6I51_RS13570 (I6I51_13575) rlmB 2674620..2675369 (+) 750 WP_004978746.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  I6I51_RS13575 (I6I51_13580) - 2675402..2676319 (+) 918 WP_201702301.1 DMT family transporter -
  I6I51_RS13580 (I6I51_13585) coaE 2676332..2676931 (-) 600 WP_201702303.1 dephospho-CoA kinase -
  I6I51_RS13585 (I6I51_13590) pilD 2676938..2677798 (-) 861 WP_176537049.1 A24 family peptidase Machinery gene
  I6I51_RS13590 (I6I51_13595) pilC 2677798..2679024 (-) 1227 WP_201702305.1 type II secretion system F family protein Machinery gene
  I6I51_RS13595 (I6I51_13600) pilB 2679055..2680773 (-) 1719 WP_201702307.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6I51_RS13600 (I6I51_13605) tpiA 2681053..2681844 (+) 792 WP_201702309.1 triose-phosphate isomerase -
  I6I51_RS13605 (I6I51_13610) secG 2681858..2682187 (+) 330 WP_004692710.1 preprotein translocase subunit SecG -
  I6I51_RS13635 (I6I51_13640) rimP 2683141..2683665 (+) 525 WP_004692708.1 ribosome maturation factor RimP -
  I6I51_RS13640 (I6I51_13645) nusA 2683705..2685189 (+) 1485 WP_004692706.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 63910.30 Da        Isoelectric Point: 5.0152

>NTDB_id=527916 I6I51_RS13595 WP_201702307.1 2679055..2680773(-) (pilB) [Acinetobacter johnsonii strain FDAARGOS_1094]
MSVSNGLPKFTGFIRRLVEDGRMTAENMQQAMLSAKKAQQDIVPYLIENHKLSPQMIAETISLEFGEPVFDLSVYDSVQI
LRDLVEDKLITKHRVLPIYRRGNILYIATSNPTNMDAMDAIRFNSKMNVEAIIVEHPQLEKLIEQHFTKADNFEFEDEDI
DLDLDLEQDPQNSNDDDDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRL
ASRLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKELFMQALD
KPQGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGVNQVNVNNKVGLTFSAALKSFLRQDPDIVMVGE
IRDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVQSFNIATSVNLVIAQRLARRLCSQCKVAADIPEQSLLEM
GFTSTDIKDPNFKIYQPGGCAECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRRSGLLKVMQGV
TSLQEMNRVTSE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=527916 I6I51_RS13595 WP_201702307.1 2679055..2680773(-) (pilB) [Acinetobacter johnsonii strain FDAARGOS_1094]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGACGCATGACTGCTGAAAA
TATGCAGCAGGCAATGCTCTCTGCAAAAAAGGCTCAGCAAGACATCGTTCCTTATCTGATTGAAAATCATAAACTCTCCC
CACAAATGATCGCTGAAACGATTTCTTTAGAGTTTGGCGAGCCTGTTTTTGACTTAAGTGTCTATGATTCGGTACAAATT
CTACGGGATCTGGTCGAAGACAAACTCATCACCAAACATCGTGTACTACCAATTTACCGTCGAGGCAATATCCTTTATAT
CGCAACCAGCAATCCAACCAATATGGATGCGATGGACGCGATTCGCTTCAATTCTAAAATGAACGTCGAAGCAATCATTG
TTGAACATCCTCAGCTTGAGAAATTGATTGAGCAACACTTTACTAAAGCAGATAACTTTGAGTTCGAAGATGAAGATATT
GATCTAGATTTAGACTTAGAACAAGACCCTCAAAACTCAAATGATGACGATGATGACTCACCTCAAGGGGATGAAGCACC
GATCGTTAAATACATCAATAAATTACTGATCGATGCGATTCGTATGGGCGCCTCAGACTTACACTTTGAACCTTATGAAA
AATCGTATCGTGTTCGTTATCGTGTAGATGGTGTTCTGCGTCATATTGCCAGTCCACCACTTCAACTGGCAACTCGTCTT
GCCTCTCGTTTGAAAGTCATGTCACAAATGGACATTTCTGAAAAGCGCGTCCCGCAAGATGGCCGGATTAAATTAAAGCT
ATCGAAAAGTAAAGCCATTGACTTTCGTGTCAACTCACTTCCAACCTTATTTGGTGAAAAACTGGTTTTGCGTATTCTCG
ACCCATCTAGTGCTATGCTGGGGATTGATGCTTTAGGTTATGAACCAGAGCAAAAAGAGCTGTTTATGCAGGCTTTAGAC
AAACCACAAGGTATGCTGCTAATTACTGGCCCGACGGGTTCAGGGAAAACGGTGTCGCTGTATACAGGTTTGAATATTCT
AAATAAAGAAGACACCAATATCTCAACTGCGGAAGATCCCGTTGAGATTAACTTAGAAGGGGTTAATCAAGTCAACGTGA
ACAATAAAGTTGGACTGACCTTTTCTGCAGCACTAAAGTCCTTCTTACGTCAAGATCCAGATATTGTGATGGTGGGTGAG
ATCCGTGACCTAGAAACGGCTGAAATTGCAATTAAAGCCGCACAGACAGGTCACATGGTCATGTCGACTCTCCACACCAA
TAGCGCACCTGAAACCCTGACACGCCTCCGTAATATGGGGGTTCAGTCTTTTAACATTGCAACATCGGTCAATTTGGTGA
TTGCGCAACGTTTGGCACGCCGTTTATGTAGCCAATGCAAAGTCGCTGCCGACATTCCCGAACAAAGTTTGCTCGAAATG
GGTTTTACGTCCACAGATATCAAAGACCCAAACTTCAAAATTTATCAACCAGGGGGATGTGCTGAGTGCCGTGAAGGCTA
TAAAGGACGTGTTGGTATTTATGAAGTCATGAAAGTCACGCCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCT
TGCAGATTGCGGAAGCATCTGCAAAATTAGGATTTAACAATTTACGTCGCTCAGGTTTACTCAAGGTTATGCAGGGGGTC
ACTTCCTTACAAGAAATGAATCGTGTAACCAGTGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.07

100

0.801
  pilB Acinetobacter baylyi ADP1

77.797

100

0.778
  pilB Legionella pneumophila strain ERS1305867

52.996

93.357

0.495
  pilF Neisseria gonorrhoeae MS11

48.936

98.601

0.483
  pilB Vibrio cholerae strain A1552

49.107

97.902

0.481
  pilB Vibrio campbellii strain DS40M4

47.415

98.077

0.465
  pilB Vibrio parahaemolyticus RIMD 2210633

51.06

90.734

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

34.249

100

0.371
  pilF Thermus thermophilus HB27

35.951

100

0.36