Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JJL50_RS16965 Genome accession   NZ_CP067993
Coordinates   3649040..3650767 (-) Length   575 a.a.
NCBI ID   WP_182266599.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain DHHJ     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3644040..3655767
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JJL50_RS16950 (JJL50_16950) sucC 3644267..3645436 (-) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -
  JJL50_RS16955 (JJL50_16955) - 3645842..3647455 (+) 1614 WP_032128821.1 ATP-binding protein -
  JJL50_RS16960 (JJL50_16960) pilR 3647516..3648889 (+) 1374 WP_010481514.1 sigma-54 dependent transcriptional regulator Regulator
  JJL50_RS16965 (JJL50_16965) pilB 3649040..3650767 (-) 1728 WP_182266599.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JJL50_RS16970 (JJL50_16970) - 3651397..3652629 (-) 1233 WP_201117184.1 isoprenylcysteine carboxylmethyltransferase family protein -
  JJL50_RS16975 (JJL50_16975) - 3652636..3653088 (-) 453 WP_201117186.1 pilin -
  JJL50_RS16980 (JJL50_16980) pilC 3653450..3654709 (+) 1260 WP_107432143.1 type II secretion system F family protein Machinery gene
  JJL50_RS16985 (JJL50_16985) - 3654718..3655581 (+) 864 WP_032128827.1 A24 family peptidase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62218.17 Da        Isoelectric Point: 6.1552

>NTDB_id=526205 JJL50_RS16965 WP_182266599.1 3649040..3650767(-) (pilB) [Stenotrophomonas maltophilia strain DHHJ]
MSVVSPPNLVGITGLARRLVQDGALDEASARDAMAKATAARQPLPTWFAQNKLVSASQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHALDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTIGDALGGD
DEGMANLDVGLGDEDNAGGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVKL
NQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLFH
DAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGNCKRPANLPEHA
LLAEGFTQAQLDAGIQLYEAVGCDECTEGYKGRTGIYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKKAA
AGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=526205 JJL50_RS16965 WP_182266599.1 3649040..3650767(-) (pilB) [Stenotrophomonas maltophilia strain DHHJ]
ATGAGCGTCGTTTCCCCCCCCAACCTCGTCGGCATCACCGGCCTGGCCCGCCGCTTGGTCCAGGACGGCGCCTTGGACGA
GGCCTCCGCCCGCGATGCCATGGCCAAGGCCACGGCCGCCCGGCAGCCGCTGCCCACGTGGTTCGCGCAGAACAAGCTGG
TCAGCGCCTCGCAGCTCGCCGCTGCCAACGCGGTCGAGTTCGGCATGCCCTTGTTCGACGTGTCTACGTTCGACGCCAGC
CAGAACGCCATGAGCCTGGTCAGCGAGGAACTGCTGCGCAAACACAACGTGCTGCCGCTGTTCAAGCGCGGTGGCAAGCT
GTTCGTAGGTACCAGCAACCCAACCCATGCACTGGACGAGATCAAGTTCCACACCAATCTGGTGGTGGAGCCGATCCTGG
TGGATGAAGACCAGATCCGCCGCACGCTGGAGCAGTGGCACGCCAGCCACGACACCATCGGCGATGCGCTGGGCGGCGAC
GACGAGGGCATGGCCAACCTCGACGTTGGACTGGGTGACGAAGACAACGCGGGGGGCGACTCCGGCATCGACGCCAAGGG
CGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGATGCCATCCGCAAGGGCGCATCGGACATCCACTTCG
AGCCCTACGAAGACGATTACCGCGTGCGCCTGCGCATCGACGGTCTGCTGAAGATGGTGGCGCGCGCGCCGGTGAAGTTG
AACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGATATCGCCGAGAAGCGCGTGCCGCAGGACGGCCGCAT
CAAGCTCAACCTTTCCAAGAGCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGTGAAAAGGTGGTGC
TGCGTATCCTGGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAGCAGAAGCTGTTCCAC
GACGCCATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCAACCGGTTCGGGCAAGACGGTGTCGCTGTATACCGC
GCTGGGCATCCTTAACGATGAGACGCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATTCGCTTGCCCGGCGTCAACC
AGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCTGCGGCACTGCGCTCGTTCCTGCGACAGGATCCGGACATCATC
ATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCTGTCCAC
GCTGCATACCAACGATGCGCCGCAGACCATCGCGCGTCTGATGAACATGGGTATCGCGCCGTACAACATCACCAGCTCGG
TGACGCTGGTGATCGCGCAGCGCCTGGCGCGCCGGTTGTGCGGCAACTGCAAGCGACCGGCCAACCTGCCGGAGCACGCG
CTGCTGGCCGAGGGCTTCACTCAGGCGCAGCTGGATGCGGGCATCCAGTTGTACGAGGCGGTGGGCTGCGATGAGTGCAC
CGAGGGCTACAAGGGCCGAACCGGTATCTACCAGGTGATGCCGATGACCGATGAGATCGCCACCATCGTGCTGGCCGGCG
GCAACGCACTGCAGATTGCCGAGGCGGCGCAGCAGATCGGGGTGAATGACCTGCGGCAGTCGGCGTTGAAGAAGGCGGCA
GCGGGTGTGACCAGCCTGGCCGAGATCAATCGCGTTACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.979

100

0.562
  pilB Acinetobacter baumannii D1279779

56.966

98.609

0.562
  pilB Legionella pneumophila strain ERS1305867

51.489

99.304

0.511
  pilB Vibrio cholerae strain A1552

48.601

99.478

0.483
  pilF Neisseria gonorrhoeae MS11

48.854

98.609

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

45.884

99.304

0.456
  pilB Vibrio campbellii strain DS40M4

44.991

98.957

0.445
  pilB/pilB1 Synechocystis sp. PCC 6803

36.007

100

0.383
  pilF Thermus thermophilus HB27

37.589

98.087

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.645

94.957

0.367