Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JHW41_RS05605 Genome accession   NZ_CP067396
Coordinates   1297429..1299156 (-) Length   575 a.a.
NCBI ID   WP_250449290.1    Uniprot ID   -
Organism   Lysobacter enzymogenes strain CX06     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1292429..1304156
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHW41_RS05585 (JHW41_05555) - 1293495..1294412 (+) 918 WP_250449288.1 Nudix family hydrolase -
  JHW41_RS05590 (JHW41_05560) coaE 1294631..1295245 (-) 615 WP_250449289.1 dephospho-CoA kinase -
  JHW41_RS05595 (JHW41_05565) - 1295250..1296137 (-) 888 WP_057948799.1 A24 family peptidase -
  JHW41_RS05600 (JHW41_05570) pilC 1296157..1297425 (-) 1269 WP_078998160.1 type II secretion system F family protein Machinery gene
  JHW41_RS05605 (JHW41_05575) pilB 1297429..1299156 (-) 1728 WP_250449290.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JHW41_RS05610 (JHW41_05580) - 1299234..1300940 (-) 1707 WP_250449291.1 phosphoethanolamine transferase -
  JHW41_RS05615 (JHW41_05585) - 1300999..1301439 (-) 441 WP_284499531.1 pilin -
  JHW41_RS05625 (JHW41_05590) pilR 1302039..1303457 (-) 1419 WP_250449292.1 sigma-54 dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62686.02 Da        Isoelectric Point: 6.3076

>NTDB_id=525863 JHW41_RS05605 WP_250449290.1 1297429..1299156(-) (pilB) [Lysobacter enzymogenes strain CX06]
MSVATTANLVGITGIVRRLVLDGALEDARAREALAAATSERKPIAAYLTEHRLVTPNQLAAAHSVEFGVPLFDPSAMDPG
QAAVRMIGEDLIRKHNALPLFKRGNKIFIGLSDPTNTSALDEIKFQTNAAVEAILVDEDLIRRTLDRWLETSDSLGDTVG
DGEGLETLEIGKDDDLSTKGDSGIDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYETDYRVRLRIDGLLKQVAKVPNK
LHSRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRMSTLPTLFGEKIVLRILDGSAARLGIEKLGYEDDQKELF
VGAVVKPYGMVLVTGPTGSGKTVSLYTALNILNDEQRNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPYNITSSVTLVIAQRLARRLHDCKREVHLPEHA
LLAEGFTHEEVAAGITLFEPVGCPDCTEGYKGRTGIYQVMPMTDEIQAIVLEGGNAMQIAAAATRSGVRDLRRSALVKVL
NGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=525863 JHW41_RS05605 WP_250449290.1 1297429..1299156(-) (pilB) [Lysobacter enzymogenes strain CX06]
ATGTCCGTGGCAACGACAGCCAACCTGGTCGGCATCACCGGCATCGTCCGGCGCTTGGTGCTGGACGGCGCCCTGGAGGA
CGCGCGGGCCCGCGAAGCCTTGGCCGCGGCGACCAGCGAGCGCAAGCCGATCGCCGCCTATCTCACCGAACACCGGCTGG
TCACGCCGAACCAGCTCGCGGCCGCCCACTCGGTCGAGTTCGGCGTGCCGTTGTTCGATCCCAGCGCGATGGATCCGGGC
CAGGCGGCCGTGCGCATGATCGGCGAAGACCTGATCCGCAAGCACAACGCCCTGCCGTTGTTCAAACGCGGCAACAAGAT
CTTCATCGGCCTGTCCGATCCGACCAACACCTCCGCGCTGGACGAGATCAAGTTCCAGACCAACGCCGCGGTCGAGGCGA
TCCTGGTCGACGAGGACCTGATCCGGCGCACGCTCGACCGCTGGCTGGAGACCTCCGACTCGCTCGGCGACACGGTCGGC
GACGGCGAAGGCCTGGAAACGCTGGAAATCGGCAAGGACGACGACCTGTCGACCAAGGGCGACAGCGGCATCGACGCCAA
GGGCGACGACACTCCCGTCGTCAAGTTCGTCAACAAGGTGTTGGTCGACGCGATCCGCCGCGGCGCGTCCGACATCCATT
TCGAGCCTTACGAAACCGACTACCGCGTGCGCCTGCGCATCGACGGCCTGCTCAAGCAGGTCGCCAAGGTGCCCAACAAG
CTGCACTCGCGCATCGCCGCGCGCCTGAAGGTCATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGCCG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCATGAGCACCTTGCCGACCCTGTTCGGCGAGAAGATCG
TGCTGCGTATCCTCGACGGCTCCGCCGCCCGCCTGGGCATCGAGAAGCTCGGCTACGAGGACGACCAGAAGGAGCTGTTC
GTCGGCGCGGTGGTCAAGCCCTACGGCATGGTCCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTACAC
CGCGCTCAACATCCTCAACGACGAGCAGCGCAACATCTCAACGGTCGAGGACCCGGTCGAAATCCGCGTGCCGGGCATCA
ACCAGGTGCAGATGAACGTCAAGCGCGGCATGACCTTCGCCGCCGCGCTGCGCAGCTTCCTGCGCCAGGACCCGGACGTG
ATCATGGTCGGCGAAATCCGCGACCTCGAGACCGCCGAAATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCTGTC
CACCCTGCACACCAACGACGCCCCGCAGACCATCGCACGCCTCATGAACATGGGCGTGGCGCCGTACAACATCACCTCCT
CGGTGACCCTGGTGATCGCCCAGCGACTGGCCCGGCGCCTGCACGACTGCAAGCGCGAAGTGCATCTGCCGGAACATGCG
CTGCTGGCCGAAGGCTTCACCCACGAGGAAGTCGCCGCCGGCATCACCCTGTTCGAACCGGTCGGCTGCCCCGACTGCAC
CGAAGGCTACAAGGGACGTACCGGCATCTACCAGGTCATGCCGATGACCGACGAAATCCAGGCGATCGTGCTCGAAGGCG
GCAACGCGATGCAGATCGCCGCCGCCGCGACGAGGTCGGGCGTGCGCGACCTGCGCCGTTCGGCGCTGGTCAAGGTGCTC
AACGGCGTCACCAGCCTGGCCGAAATCAACCGCGTAACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.44

100

0.558
  pilB Acinetobacter baumannii D1279779

55.282

98.783

0.546
  pilB Legionella pneumophila strain ERS1305867

50.088

99.304

0.497
  pilB Vibrio cholerae strain A1552

48.776

99.478

0.485
  pilF Neisseria gonorrhoeae MS11

46.667

99.13

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

48.479

91.478

0.443
  pilB Vibrio campbellii strain DS40M4

51.129

84.696

0.433
  pilB/pilB1 Synechocystis sp. PCC 6803

37.108

99.826

0.37
  pilF Thermus thermophilus HB27

39.279

91.652

0.36