Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JHT90_RS04080 Genome accession   NZ_CP067393
Coordinates   900279..901943 (-) Length   554 a.a.
NCBI ID   WP_201094476.1    Uniprot ID   A0A974NGQ6
Organism   Entomomonas asaccharolytica strain F2A     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 895279..906943
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHT90_RS04060 (JHT90_04060) yacG 897102..897320 (-) 219 WP_201094467.1 DNA gyrase inhibitor YacG -
  JHT90_RS04065 (JHT90_04065) coaE 897325..897927 (-) 603 WP_201094471.1 dephospho-CoA kinase -
  JHT90_RS04070 (JHT90_04070) pilD 898015..898890 (-) 876 WP_379971860.1 prepilin peptidase Machinery gene
  JHT90_RS04075 (JHT90_04075) pilC 898887..900095 (-) 1209 WP_201094475.1 type II secretion system F family protein Machinery gene
  JHT90_RS04080 (JHT90_04080) pilB 900279..901943 (-) 1665 WP_201094476.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JHT90_RS04085 (JHT90_04085) - 902109..903089 (-) 981 WP_201094479.1 HD domain-containing phosphohydrolase -
  JHT90_RS04090 (JHT90_04090) - 903094..905700 (-) 2607 WP_201094481.1 PAS domain S-box protein -

Sequence

Protein


Download         Length: 554 a.a.        Molecular weight: 61150.14 Da        Isoelectric Point: 5.3127

>NTDB_id=525819 JHT90_RS04080 WP_201094476.1 900279..901943(-) (pilB) [Entomomonas asaccharolytica strain F2A]
MSAQNPPLTGLPRLFVDNDILSENDVRQAMQQSKVEQLPLVTYLVQNKMASAKSIAILISEQLGLPLFDLGSLQIDDALK
SVVTEKLARQHRAIPLWKRGNSLFLGISDPSNQQGLRDIQFNTGLPVDAILVEEDKLNSLLDRFFDTEAEQVSAGPLQKP
GVGDDEKEDEAVVNFVNQMLLNAIRSGASDLHFEPYEKSYRVRFRSDGILQIKSRPPIELAPRIAARLKVMSDMDISERR
KPQDGRIKLKVSERKSIDFRVNTCPTLWGEKIVLRILDSSSAQMGIDSLGYEPDQKALYLEALSHPQGMILVTGPTGSGK
TVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPKQGMDFAAALRAFLRQDPDVIMVGEIRDLETAEIAIKAAQT
GHMVMSTLHTNSAPETLTRLMNMGVASFNIATSVNLIIAQRLARRLCPHCKQEMEIPHEALIKAGFPQDKIGNFTVYGPL
GCDRCNNGYKGRVGIYEVVKITPALQRIIMSEGNAIEIADEARKEGFPDLRASGLKKVMDGLTSLEEVNRVTSD

Nucleotide


Download         Length: 1665 bp        

>NTDB_id=525819 JHT90_RS04080 WP_201094476.1 900279..901943(-) (pilB) [Entomomonas asaccharolytica strain F2A]
ATGAGCGCACAAAATCCTCCTTTAACTGGACTTCCAAGGCTGTTTGTAGATAATGATATCTTATCAGAAAATGATGTGCG
CCAAGCCATGCAACAATCTAAGGTAGAACAATTACCGCTTGTTACTTATTTAGTACAGAATAAAATGGCTAGCGCGAAAA
GCATTGCTATACTTATCTCAGAACAACTTGGATTGCCTCTTTTTGATTTAGGCAGTCTACAAATAGATGATGCATTAAAA
TCTGTAGTTACTGAAAAACTGGCTCGGCAACATCGCGCAATCCCCTTATGGAAACGTGGCAACAGTCTCTTCTTAGGTAT
TTCTGACCCATCTAATCAGCAAGGCTTGAGAGATATCCAATTTAATACTGGTTTACCTGTTGATGCGATTCTTGTTGAAG
AAGATAAATTAAACTCTTTACTTGATCGTTTCTTTGATACTGAAGCAGAACAAGTCTCTGCTGGGCCTTTGCAAAAACCT
GGCGTAGGGGATGATGAAAAAGAAGACGAAGCTGTGGTTAATTTTGTTAATCAAATGCTATTGAATGCTATACGTAGCGG
TGCATCTGACTTACATTTTGAACCTTATGAAAAAAGTTATAGAGTACGTTTCCGCAGTGACGGTATCCTACAAATAAAAT
CTCGCCCTCCTATTGAATTAGCACCTCGTATTGCAGCACGTTTAAAAGTAATGTCTGATATGGATATTTCTGAACGTAGA
AAGCCTCAAGATGGACGGATTAAATTAAAAGTATCAGAAAGAAAATCTATTGACTTCCGGGTTAATACTTGCCCTACACT
CTGGGGTGAAAAAATAGTATTACGTATACTAGATTCCTCTAGCGCCCAAATGGGTATTGATTCACTTGGTTATGAACCTG
ACCAAAAAGCACTCTATTTAGAAGCCTTAAGTCACCCTCAAGGTATGATTTTAGTTACAGGACCAACAGGTTCTGGTAAA
ACTGTATCTCTCTATACGGGCTTAAATATCCTTAATACTCCCGATATTAATATCTCCACAGCAGAAGATCCTGTTGAAAT
TAACTTAGAAGGGATAAATCAAGTAAACGTTAATCCCAAACAGGGAATGGATTTCGCCGCAGCATTACGGGCATTCTTAC
GTCAGGATCCTGATGTTATCATGGTGGGTGAGATCCGTGACCTTGAAACTGCTGAAATTGCGATTAAAGCAGCACAAACA
GGTCATATGGTAATGTCTACACTGCATACCAATAGTGCTCCTGAAACATTAACTCGTTTGATGAATATGGGTGTCGCTTC
ATTTAATATTGCAACCTCTGTTAACTTAATTATTGCGCAACGATTGGCTAGGCGTTTATGCCCACATTGTAAACAGGAAA
TGGAAATTCCTCATGAAGCTTTAATAAAAGCAGGATTTCCTCAAGATAAAATCGGCAACTTCACTGTTTATGGCCCTTTA
GGTTGTGATCGTTGTAACAATGGTTACAAAGGTCGTGTGGGTATTTATGAAGTAGTAAAAATAACCCCTGCGTTACAACG
TATTATTATGTCTGAAGGTAATGCTATTGAAATTGCTGATGAGGCAAGAAAAGAAGGTTTTCCTGATTTAAGAGCTTCTG
GCCTCAAAAAAGTAATGGATGGTTTAACTAGTTTAGAAGAGGTAAATCGTGTTACATCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(178-445)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.123

100

0.608
  pilB Acinetobacter baylyi ADP1

58.615

100

0.596
  pilB Legionella pneumophila strain ERS1305867

50.874

100

0.525
  pilF Neisseria gonorrhoeae MS11

50.268

100

0.507
  pilB Vibrio cholerae strain A1552

48.929

100

0.495
  pilB Vibrio campbellii strain DS40M4

48.128

100

0.487
  pilB Vibrio parahaemolyticus RIMD 2210633

49.351

97.292

0.48
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.926

97.653

0.39
  pilF Thermus thermophilus HB27

38.121

100

0.388
  pilB/pilB1 Synechocystis sp. PCC 6803

39.189

93.502

0.366