Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JFY49_RS01635 Genome accession   NZ_CP067019
Coordinates   355565..357277 (-) Length   570 a.a.
NCBI ID   WP_086195207.1    Uniprot ID   A0A241VDC2
Organism   Acinetobacter sp. CS-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 350565..362277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JFY49_RS01605 (JFY49_01605) - 350662..350985 (+) 324 WP_086195201.1 pyrimidine/purine nucleoside phosphorylase -
  JFY49_RS01610 (JFY49_01610) rlmB 351127..351876 (+) 750 WP_086195202.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  JFY49_RS01615 (JFY49_01615) - 351908..352825 (+) 918 WP_086195203.1 DMT family transporter -
  JFY49_RS01620 (JFY49_01620) coaE 352845..353435 (-) 591 WP_086195204.1 dephospho-CoA kinase -
  JFY49_RS17615 (JFY49_01625) pilD 353451..354311 (-) 861 WP_086195205.1 prepilin peptidase Machinery gene
  JFY49_RS01630 (JFY49_01630) pilC 354311..355537 (-) 1227 WP_086195206.1 type II secretion system F family protein Machinery gene
  JFY49_RS01635 (JFY49_01635) pilB 355565..357277 (-) 1713 WP_086195207.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JFY49_RS01640 (JFY49_01640) tpiA 357558..358349 (+) 792 WP_086195208.1 triose-phosphate isomerase -
  JFY49_RS01645 (JFY49_01645) secG 358363..358692 (+) 330 WP_086195209.1 preprotein translocase subunit SecG -
  JFY49_RS01675 (JFY49_01675) rimP 359632..360156 (+) 525 WP_086195210.1 ribosome maturation factor RimP -
  JFY49_RS01680 (JFY49_01680) nusA 360197..361681 (+) 1485 WP_086195211.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63270.62 Da        Isoelectric Point: 4.8582

>NTDB_id=524461 JFY49_RS01635 WP_086195207.1 355565..357277(-) (pilB) [Acinetobacter sp. CS-2]
MAALQGSPRFTGFIRCLVEDGLVSAENMQSAILNAKKANQDIVPFLIDQLKISSLAIAEKISQEFGEPLFDLSAYDSAQI
IREGLDEKIISKHCILPIFKRGNLLYVATSNPTNIEAMDAIRFSSKMNIEAIIVEHQKLHQLIEQNFTQADSFEFSDEDI
DLNLEQDDLSQKEDEDDGPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRIDGVLRQIANPPLQLSTRLAS
RLKVMSQMDISEKRLPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKELFMQALEKP
QGMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNAKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAVKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKIPAEIPGPSLLEMGF
TEEDLKHPDFQVYQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKSGFNNLRRSGLIKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=524461 JFY49_RS01635 WP_086195207.1 355565..357277(-) (pilB) [Acinetobacter sp. CS-2]
ATGGCAGCATTACAAGGTTCACCAAGATTTACGGGTTTTATTCGATGTTTAGTCGAAGATGGCTTAGTTTCTGCCGAAAA
TATGCAGAGTGCAATATTGAATGCAAAAAAAGCCAATCAAGATATTGTGCCTTTTTTAATTGATCAATTGAAAATTTCCT
CCTTGGCTATTGCAGAAAAAATTTCTCAAGAATTTGGAGAACCTCTTTTTGATTTGAGTGCCTATGACTCTGCTCAAATT
ATCCGAGAAGGGCTGGATGAAAAGATCATTAGCAAACATTGTATTCTGCCCATTTTTAAACGCGGGAATTTACTCTACGT
CGCAACCAGTAATCCCACCAATATAGAAGCCATGGATGCGATCCGCTTCAGCAGTAAGATGAATATCGAAGCGATTATTG
TAGAGCACCAGAAATTACACCAGCTGATTGAACAAAACTTTACCCAGGCGGACAGTTTTGAGTTTTCTGATGAAGATATC
GATCTAAATCTAGAGCAAGATGATTTAAGTCAAAAAGAAGATGAAGATGATGGTCCTCAAGGGGATGAAGCTCCCATCGT
TAAGTACATCAATAAACTGTTAATTGATGCCATTCGTATGGGTGCTTCGGATTTACATTTCGAACCGTATGAAAAGTCAT
ATCGGGTGCGTTATCGTATTGATGGCGTGCTGCGTCAAATTGCCAATCCGCCGTTACAGCTGTCCACACGTTTAGCCTCA
CGTTTAAAAGTCATGTCGCAGATGGATATTTCTGAAAAACGCTTACCTCAGGATGGCCGGATTAAACTTAAGCTTTCAAA
AACCAAAGCCATCGACTTCCGTGTTAACTCATTACCTACCCTATTTGGGGAAAAACTGGTCCTGCGTATTCTGGATCCAT
CCAGTGCCATGCTGGGTATTGATGCATTGGGCTATGAACCAGAGCAAAAAGAACTGTTTATGCAAGCGCTGGAAAAACCT
CAAGGCATGCTACTGATTACAGGTCCGACAGGTTCCGGTAAAACCGTATCTCTCTATACGGGTTTGAATATTCTGAATCG
TGAAGATACCAATATTTCCACAGCTGAAGACCCGGTAGAAATTAACCTGGAAGGGATTAATCAGGTCAATGTTAATGCTA
AAGTCGGTTTAACCTTCTCTGCTGCGCTTAAATCATTTTTACGTCAAGATCCAGATATTGTCATGGTCGGTGAGATTCGA
GATCTGGAAACAGCTGAAATTGCCGTAAAAGCGGCCCAGACCGGTCATATGGTGATGTCGACCCTGCACACCAACAGTGC
ACCAGAAACCTTAACCCGTTTACGCAATATGGGTGTTCCTTCCTTTAACATTGCAACTTCTGTCAACCTAGTGATTGCAC
AACGTTTAGCACGTCGTCTATGTCCTCAGTGTAAAATTCCGGCAGAGATTCCTGGACCCAGCCTGCTGGAAATGGGCTTT
ACCGAAGAAGATTTGAAACATCCAGACTTTCAGGTATATCAACCGGTAGGCTGTTCCGAGTGCCGTGAAGGTTATAAAGG
TCGTGTCGGTATTTATGAGGTCATGAAAGTGACCCCTGAAATTTCGAAAATCATTATGGAGGATGGTAATGCACTTGAAA
TTGCAGCAGCATCTGAAAAATCGGGGTTTAATAATTTACGCCGTTCAGGATTAATTAAGGTTATGCAGGGGGTGACTTCC
CTTCAGGAAGTCAATCGTGTAACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A241VDC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80

100

0.8
  pilB Acinetobacter baylyi ADP1

76.316

100

0.763
  pilB Legionella pneumophila strain ERS1305867

52.72

93.509

0.493
  pilF Neisseria gonorrhoeae MS11

49.468

98.947

0.489
  pilB Vibrio cholerae strain A1552

48.682

99.825

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

48.929

98.246

0.481
  pilB Vibrio campbellii strain DS40M4

47.535

99.649

0.474
  pilB/pilB1 Synechocystis sp. PCC 6803

34.41

100

0.374
  pilF Thermus thermophilus HB27

36.555

99.825

0.365