Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6H70_RS16980 Genome accession   NZ_CP067013
Coordinates   3644139..3645842 (+) Length   567 a.a.
NCBI ID   WP_200290946.1    Uniprot ID   A0A9X7V2V1
Organism   Stutzerimonas balearica strain FDAARGOS_1013     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3639139..3650842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6H70_RS16960 (I6H70_16960) cysD 3639425..3640342 (+) 918 WP_200290937.1 sulfate adenylyltransferase subunit CysD -
  I6H70_RS16965 (I6H70_16965) cysN 3640355..3642253 (+) 1899 WP_200290940.1 sulfate adenylyltransferase subunit CysN -
  I6H70_RS16970 (I6H70_16970) - 3642353..3642771 (-) 419 Protein_3348 transposase -
  I6H70_RS16975 (I6H70_16975) - 3643427..3643912 (-) 486 WP_200290943.1 pilin -
  I6H70_RS16980 (I6H70_16980) pilB 3644139..3645842 (+) 1704 WP_200290946.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6H70_RS16985 (I6H70_16985) pilC 3645845..3647062 (+) 1218 WP_200290949.1 type II secretion system F family protein Machinery gene
  I6H70_RS16990 (I6H70_16990) pilD 3647062..3647931 (+) 870 WP_200290952.1 A24 family peptidase Machinery gene
  I6H70_RS16995 (I6H70_16995) coaE 3648025..3648633 (+) 609 WP_200290955.1 dephospho-CoA kinase -
  I6H70_RS17000 (I6H70_17000) yacG 3648630..3648827 (+) 198 WP_074519929.1 DNA gyrase inhibitor YacG -
  I6H70_RS17005 (I6H70_17005) - 3648821..3649531 (-) 711 WP_200290958.1 energy-coupling factor ABC transporter permease -
  I6H70_RS17010 (I6H70_17010) rplM 3649898..3650326 (+) 429 WP_041104003.1 50S ribosomal protein L13 -
  I6H70_RS17015 (I6H70_17015) rpsI 3650341..3650733 (+) 393 WP_041104005.1 30S ribosomal protein S9 -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62039.05 Da        Isoelectric Point: 5.5861

>NTDB_id=524392 I6H70_RS16980 WP_200290946.1 3644139..3645842(+) (pilB) [Stutzerimonas balearica strain FDAARGOS_1013]
MTDNVALSGLAKQMVLAGLLDDRTAQQAHQQAVRNKLSLVTYVVQNKLASGRAVAELAADQFGVAYMDLNALDKESQLKD
VVSEKLARQHRVLPLVKRGHKLFVAVSDPTNHQAVTDIQFSTGLTTEAILVEDDKLGNAIDKFFESATTGLDDLENVDLD
GVDVQTASDDAPSDDAGDAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRFRTDGILHEIARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEAQKELYLAALSQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCSTCKKEVDVPREALLREGFP
EDKIGTFKIYAPVGCDNCKGGYKGRVGIYEVVKITPALQRIIMEDGNSLDISLQMRKDGFNDLRTSALLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=524392 I6H70_RS16980 WP_200290946.1 3644139..3645842(+) (pilB) [Stutzerimonas balearica strain FDAARGOS_1013]
ATGACTGACAACGTTGCCCTCTCCGGCCTGGCCAAGCAGATGGTGCTGGCCGGCCTGCTCGATGACCGGACGGCTCAGCA
AGCACACCAGCAAGCCGTGCGAAACAAACTGTCGTTGGTGACCTATGTGGTCCAGAACAAGCTCGCCAGCGGGCGTGCCG
TGGCGGAGCTGGCGGCTGACCAGTTCGGCGTGGCCTATATGGACCTCAATGCACTGGACAAGGAAAGCCAGTTAAAGGAT
GTGGTATCCGAAAAGCTGGCGCGACAGCATCGGGTGCTCCCTTTGGTCAAACGGGGCCATAAGCTGTTTGTGGCGGTGTC
CGACCCTACCAACCATCAGGCCGTAACGGATATTCAGTTCAGCACCGGACTGACCACCGAGGCCATCCTTGTTGAAGATG
ACAAACTCGGGAATGCCATTGATAAGTTCTTCGAAAGCGCCACGACGGGGCTGGATGACTTAGAGAATGTCGATCTTGAT
GGGGTTGACGTCCAGACCGCCTCGGATGATGCACCCAGCGATGATGCCGGAGACGCCGCAGACGATGCTCCGGTCGTCCG
CTTCGTTAATAAGATGTTGCTCGACGCCATTCGCGGCGGCTCGTCAGACCTGCACTTCGAGCCGTATGAGAAGGCCTACC
GGGTCCGCTTCCGCACCGATGGCATCCTTCACGAGATCGCTCGCCCGCCCATCCAGCTCGCGCCTCGCATTTCTGCGCGG
CTGAAGGTGATGGCTGGGCTGGATATTTCCGAGCGGCGCAAGCCGCAGGACGGCCGGATCAAGATGAAGCTGTCCAAGAC
CAAGGCCATCGATTTTCGCGTGAATACCTTGCCCACCTTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACCCAACCA
GTGCGCAGATGGGCATCGATGCGCTGGGTTATGAGGAAGCGCAGAAGGAGCTGTACCTCGCTGCGTTGAGCCAGCCGCAG
GGCATGATCCTGGTGACCGGGCCGACCGGCTCGGGCAAGACCGTTTCGCTCTATACCGGGCTTAATATCCTCAACACCGT
CGACGTCAATATCTCTACGGCCGAGGACCCGGTGGAAATCAACCTGGAAGGCATCAACCAGGTCAACGTGAATCCCAAGC
AGGGCATGGACTTTTCCCAGGCGCTGCGCGCCTTCCTGCGTCAGGACCCGGACATCATCATGGTCGGCGAGATTCGCGAC
CTGGAAACGGCGGAGATTGCCATCAAGGCGGCGCAGACGGGGCACATGGTGATGTCGACCCTGCACACCAACAGCGCGGC
GGAAACCCTGACGCGCCTGCGCAACATGGGCGTGCCGGCGTTCAACATCGCCACCTCGGTCAATCTGATCATCGCCCAGC
GCCTGGCGCGCAAGCTTTGCAGTACCTGCAAGAAGGAAGTCGACGTGCCGCGCGAGGCGTTGCTGCGCGAGGGCTTTCCT
GAAGACAAGATCGGCACCTTCAAGATCTACGCGCCGGTGGGCTGCGATAACTGCAAGGGCGGCTACAAAGGCCGCGTCGG
CATTTATGAAGTAGTTAAAATCACGCCGGCTCTGCAGCGCATTATCATGGAAGACGGAAATTCGCTGGATATTTCTCTCC
AGATGCGTAAGGACGGCTTCAATGACCTGCGAACATCGGCTTTGTTGAAGGCCATGCAGGGCGTGACCAGCCTCGAAGAA
GTCAACCGGGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

60.816

99.471

0.605
  pilB Acinetobacter baumannii D1279779

59.788

100

0.598
  pilB Legionella pneumophila strain ERS1305867

55.614

100

0.559
  pilF Neisseria gonorrhoeae MS11

52.682

92.063

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

48.582

99.471

0.483
  pilB Vibrio campbellii strain DS40M4

47.965

99.647

0.478
  pilB Vibrio cholerae strain A1552

52.539

90.3

0.474
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.037

98.942

0.386
  pilF Thermus thermophilus HB27

37.097

98.413

0.365
  pilB/pilB1 Synechocystis sp. PCC 6803

39.731

91.887

0.365