Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssb   Type   Machinery gene
Locus tag   EFD32_RS09735 Genome accession   NC_018221
Coordinates   1939148..1939639 (-) Length   163 a.a.
NCBI ID   WP_014862267.1    Uniprot ID   -
Organism   Enterococcus faecalis D32     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1934074..1955102 1939148..1939639 within 0


Gene organization within MGE regions


Location: 1934074..1955102
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFD32_RS09690 (EFD32_1860) - 1934294..1934659 (+) 366 WP_014862256.1 type II toxin-antitoxin system PemK/MazF family toxin -
  EFD32_RS09695 (EFD32_1861) - 1935233..1935682 (+) 450 WP_014862257.1 hypothetical protein -
  EFD32_RS09700 (EFD32_1862) - 1935698..1936084 (+) 387 WP_014862258.1 hypothetical protein -
  EFD32_RS09705 (EFD32_1863) - 1936712..1937074 (+) 363 WP_014862259.1 hypothetical protein -
  EFD32_RS09710 (EFD32_1864) - 1937274..1937462 (-) 189 WP_014862260.1 hypothetical protein -
  EFD32_RS09715 (EFD32_1865) - 1937592..1937846 (-) 255 WP_014862261.1 hypothetical protein -
  EFD32_RS09720 (EFD32_1867) - 1937971..1938207 (-) 237 WP_014862263.1 hypothetical protein -
  EFD32_RS09725 (EFD32_1868) - 1938194..1938370 (-) 177 WP_181035826.1 hypothetical protein -
  EFD32_RS09730 (EFD32_1870) - 1938492..1939052 (-) 561 Protein_1850 thermonuclease family protein -
  EFD32_RS09735 (EFD32_1871) ssb 1939148..1939639 (-) 492 WP_014862267.1 single-stranded DNA-binding protein Machinery gene
  EFD32_RS15910 - 1939886..1941278 (+) 1393 Protein_1852 IS3 family transposase -
  EFD32_RS09750 - 1941868..1943284 (-) 1417 Protein_1853 IS1182 family transposase -
  EFD32_RS09765 (EFD32_1879) - 1944864..1945238 (-) 375 Protein_1855 replication protein RepA -
  EFD32_RS16510 (EFD32_1880) - 1945413..1945577 (+) 165 WP_154231859.1 hypothetical protein -
  EFD32_RS16340 - 1945815..1945916 (-) 102 WP_373408712.1 type I toxin-antitoxin system Fst family toxin -
  EFD32_RS09780 (EFD32_1881) istB 1946245..1947000 (-) 756 WP_010709161.1 IS21-like element helper ATPase IstB -
  EFD32_RS15915 istA 1947013..1948231 (-) 1219 Protein_1859 IS21 family transposase -
  EFD32_RS09800 (EFD32_1884) yajC 1948541..1948852 (+) 312 WP_014862279.1 preprotein translocase subunit YajC -
  EFD32_RS15920 - 1948752..1948994 (-) 243 WP_238535493.1 hypothetical protein -
  EFD32_RS16695 (EFD32_1885) - 1949391..1949528 (+) 138 WP_014862280.1 DUF3784 domain-containing protein -
  EFD32_RS09810 (EFD32_1886) - 1949836..1951167 (-) 1332 WP_014862281.1 IS1182 family transposase -
  EFD32_RS09815 (EFD32_1887) - 1951712..1952164 (-) 453 WP_014862282.1 hypothetical protein -
  EFD32_RS09825 (EFD32_1889) - 1953255..1953635 (-) 381 WP_014862284.1 hypothetical protein -
  EFD32_RS09830 (EFD32_1890) - 1953690..1955102 (-) 1413 WP_014862285.1 oligosaccharide flippase family protein -

Sequence

Protein


Download         Length: 163 a.a.        Molecular weight: 18436.41 Da        Isoelectric Point: 5.2339

>NTDB_id=52275 EFD32_RS09735 WP_014862267.1 1939148..1939639(-) (ssb) [Enterococcus faecalis D32]
MMNNVTLIGRATKDPDLRYTASGIAVATFTLAVNRNYKNQNGDREADFINCVIWRKPAETFANYVRKGTLVGIVGRIQTR
FYENQQGQRVYVTEVIVESYQLLESRSQAEAREQKQRTITNQNPPMNNTTDNISNHSSATNSMPNDDPFDGNPINISDDD
LPF

Nucleotide


Download         Length: 492 bp        

>NTDB_id=52275 EFD32_RS09735 WP_014862267.1 1939148..1939639(-) (ssb) [Enterococcus faecalis D32]
ATGATGAATAATGTGACATTAATTGGAAGAGCAACAAAAGATCCAGATTTACGTTATACAGCTAGTGGAATAGCTGTTGC
AACGTTTACTTTAGCTGTAAATCGTAATTATAAAAACCAAAATGGTGATCGTGAAGCAGACTTTATCAACTGTGTGATTT
GGCGTAAGCCGGCGGAGACGTTTGCGAACTATGTTAGAAAAGGAACGTTGGTTGGAATAGTAGGAAGAATTCAAACTCGA
TTTTATGAAAATCAACAAGGTCAGCGTGTTTATGTAACAGAAGTAATCGTGGAAAGTTATCAGTTGTTAGAATCACGTTC
TCAAGCAGAAGCAAGAGAACAAAAACAAAGAACGATAACGAATCAGAATCCACCTATGAATAATACTACAGACAATATAA
GTAACCATTCTTCAGCAACTAATTCAATGCCAAATGATGATCCATTTGATGGGAATCCAATAAATATTTCAGATGATGAC
TTACCGTTCTGA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

56

100

0.601
  ssbA Bacillus subtilis subsp. subtilis str. 168

53.409

100

0.577
  ssbB Bacillus subtilis subsp. subtilis str. 168

55.856

68.098

0.38
  ssbB/cilA Streptococcus pneumoniae TIGR4

53.097

69.325

0.368
  ssbB/cilA Streptococcus mitis NCTC 12261

52.212

69.325

0.362
  ssbB/cilA Streptococcus pneumoniae Rx1

52.212

69.325

0.362
  ssbB/cilA Streptococcus pneumoniae D39

52.212

69.325

0.362
  ssbB/cilA Streptococcus pneumoniae R6

52.212

69.325

0.362
  ssbB/cilA Streptococcus mitis SK321

52.212

69.325

0.362

Multiple sequence alignment