Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JH254_RS00985 Genome accession   NZ_CP066813
Coordinates   194041..195747 (+) Length   568 a.a.
NCBI ID   WP_200164279.1    Uniprot ID   -
Organism   Aeromonas caviae strain Aero52     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 189041..200747
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH254_RS00960 (JH254_00960) pdhR 190201..190965 (-) 765 WP_010675815.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  JH254_RS00965 (JH254_00965) ampD 191244..191813 (-) 570 WP_180907147.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  JH254_RS00970 (JH254_00970) - 191926..192396 (+) 471 WP_042077632.1 TIGR02281 family clan AA aspartic protease -
  JH254_RS00975 (JH254_00975) nadC 192400..193287 (+) 888 WP_200164277.1 carboxylating nicotinate-nucleotide diphosphorylase -
  JH254_RS00980 (JH254_00980) - 193597..194037 (+) 441 WP_200164278.1 pilin -
  JH254_RS00985 (JH254_00985) pilB 194041..195747 (+) 1707 WP_200164279.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  JH254_RS00990 (JH254_00990) pilC 195876..197117 (+) 1242 WP_200164280.1 type II secretion system F family protein Machinery gene
  JH254_RS00995 (JH254_00995) pilD 197185..198060 (+) 876 WP_200164281.1 prepilin peptidase Machinery gene
  JH254_RS01000 (JH254_01000) coaE 198079..198693 (+) 615 WP_109111851.1 dephospho-CoA kinase -
  JH254_RS01005 (JH254_01005) zapD 198731..199453 (+) 723 WP_010675825.1 cell division protein ZapD -
  JH254_RS01010 (JH254_01010) yacG 199464..199658 (+) 195 WP_039040499.1 DNA gyrase inhibitor YacG -
  JH254_RS01015 (JH254_01015) mutT 199714..200118 (-) 405 WP_171864957.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62621.98 Da        Isoelectric Point: 5.8204

>NTDB_id=522743 JH254_RS00985 WP_200164279.1 194041..195747(+) (pilB) [Aeromonas caviae strain Aero52]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDENSRLDDSVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNLDQLGFDDRQKGQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKTPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=522743 JH254_RS00985 WP_200164279.1 194041..195747(+) (pilB) [Aeromonas caviae strain Aero52]
ATGACCTCTAGCCTTAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCCTCACTCCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCCAGGGCGCAGCGTAAGCCCTTTGTCACCTTCCTGATTGAGAACGACATCCTCGACAGCAAGGCCCTCG
CCGACTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAACACCATGTGCTGCCCATCTACACCCAGGGGCACACACTCTATATCGCCATGTCGGA
TCCAACCAATGTGTCGGCCCTGGAGGATTTCGGCTTCAGCTTCGGTCTGCACACCGAGGCCCTGCTGGTCGAGGAGAGCA
AGCTGACCGCCGCCATCGGCAAATTGATGGAGAGCGAGCAGGATACCCTCGGCATGGATCATATCGACGAGGCCGAGATC
TCCGAGCTCGAGGTGTCCGACGAGAACTCCCGCCTCGACGACAGCGTCAATACGGCCGACGATGACGCCCCCATCGTCAA
GTACATCCACAAGATCATGATGGACGCCATCAAGCGCGGCGCATCGGATCTGCATTTCGAACCTTACGAGACCAAGTACC
GGATCCGCTTCCGGATCGACGGCATACTGCACGAGATCGCCACCCCGCCGGTGAACCTGGCGAACCGTTTCGCGGCTCGC
TTAAAAGTCATGGCGCGGCTCGACATCGCCGAACGGCGTCTGCCCCAGGATGGCCGGATCAAGCTCAAGATTTCGCGCAA
CCGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAGAAGATCGTCATCCGGCTGCTGGACTCCTCTG
CCGCCCGTCTCAACCTCGATCAGCTCGGCTTCGACGACCGCCAGAAGGGGCAATACCTGCACGCCCTCTCCAAGCCCCAG
GGGATGATCCTGGTCACCGGCCCCACGGGGTCGGGCAAGACGGTATCGCTCTATACCGGTCTCAACATCCTCAACACCAG
CGAGGTCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGCCCGGCGTGAACCAGGTGCAGATCAACCCCAAGG
CGGGGCTCACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGTCAGGATCCCGACATCGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTGTCCACCCTGCACACCAATTCGGCGGC
CGAGACGCTCACCCGGATGATGAACATGGGCGTCCCCGCCTTCAACATCGCCTCCTCGGTCACCCTCATCATGGCCCAGC
GCCTCGCCCGCAAGCTGTGCGATCATTGCAAGACGCCCGAAGTGGTGCCGGAAGCCGAGCTGCTGGAGCTGGGCTTTACC
CAGCAGCAGCTGGCCGCCGGCTTGCGGCTGTTCAAGCCCGTGGGGTGCAAGGAGTGCTCGGGAGGCTACCGGGGGCGGGT
CGGGATCTATGAGATCATGCTGATGTCAGACAACATCGCCAAGCTCATCATGCAAGGAGCCAACTCGCTGCAAATAGCGG
CCATTGCCCAGAAGGAAGGGATGCGCACCCTGCGTACCTCGGGCCTTGAAAAAGCCCGCCTGGGGGTCACCAGCCTGGCC
GAAATAAACAGGATCACTACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.885

99.12

0.544
  pilB Acinetobacter baumannii D1279779

54.497

99.824

0.544
  pilB Legionella pneumophila strain ERS1305867

54.078

99.296

0.537
  pilB Vibrio cholerae strain A1552

53.24

100

0.535
  pilB Vibrio campbellii strain DS40M4

49.911

99.12

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

49.734

99.12

0.493
  pilF Neisseria gonorrhoeae MS11

46.032

99.824

0.46
  pilF Thermus thermophilus HB27

39.962

92.958

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

37.862

97.183

0.368