Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JH254_RS00995 Genome accession   NZ_CP066813
Coordinates   197185..198060 (+) Length   291 a.a.
NCBI ID   WP_200164281.1    Uniprot ID   -
Organism   Aeromonas caviae strain Aero52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 192185..203060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH254_RS00975 (JH254_00975) nadC 192400..193287 (+) 888 WP_200164277.1 carboxylating nicotinate-nucleotide diphosphorylase -
  JH254_RS00980 (JH254_00980) - 193597..194037 (+) 441 WP_200164278.1 pilin -
  JH254_RS00985 (JH254_00985) pilB 194041..195747 (+) 1707 WP_200164279.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  JH254_RS00990 (JH254_00990) pilC 195876..197117 (+) 1242 WP_200164280.1 type II secretion system F family protein Machinery gene
  JH254_RS00995 (JH254_00995) pilD 197185..198060 (+) 876 WP_200164281.1 prepilin peptidase Machinery gene
  JH254_RS01000 (JH254_01000) coaE 198079..198693 (+) 615 WP_109111851.1 dephospho-CoA kinase -
  JH254_RS01005 (JH254_01005) zapD 198731..199453 (+) 723 WP_010675825.1 cell division protein ZapD -
  JH254_RS01010 (JH254_01010) yacG 199464..199658 (+) 195 WP_039040499.1 DNA gyrase inhibitor YacG -
  JH254_RS01015 (JH254_01015) mutT 199714..200118 (-) 405 WP_171864957.1 8-oxo-dGTP diphosphatase MutT -
  JH254_RS01020 (JH254_01020) - 200127..201311 (-) 1185 WP_200164282.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32210.45 Da        Isoelectric Point: 7.0270

>NTDB_id=522745 JH254_RS00995 WP_200164281.1 197185..198060(+) (pilD) [Aeromonas caviae strain Aero52]
MAPLIELAQALPWLYLSLVFLFSLMVGSFLNVVIHRLPIMMERDWHAEYTDYFGEDTSPIPATGRYNLMVPRSRCPHCDH
PIGALENIPLLSWLWLRGRCRNCRAPISARYPLVELLTALLSVAVAATLTPGWGTLAALVLTWVLVALTFIDLDKMLLPD
QLTLPLLWGGLLFNLPGGFVPLVDAVIGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSS
LAGAIIGIGLILLRNHHQGKPIPFGPYLAIAGWITLLWGDSITRWYLDVVL

Nucleotide


Download         Length: 876 bp        

>NTDB_id=522745 JH254_RS00995 WP_200164281.1 197185..198060(+) (pilD) [Aeromonas caviae strain Aero52]
ATGGCGCCCCTGATCGAACTGGCCCAGGCCCTGCCCTGGCTTTATCTCTCCCTGGTGTTTCTCTTCTCCCTGATGGTGGG
CAGCTTCCTCAACGTGGTGATCCATCGCCTGCCCATCATGATGGAGCGGGACTGGCACGCCGAATACACCGACTATTTCG
GTGAAGATACATCCCCCATCCCGGCCACAGGACGCTACAACCTGATGGTACCCCGCTCCCGCTGCCCCCATTGCGACCAC
CCCATTGGTGCGCTGGAGAACATCCCCCTGCTGAGCTGGCTCTGGCTCAGGGGGCGCTGCCGCAACTGCCGTGCCCCCAT
CTCCGCCCGCTATCCCCTGGTCGAGTTGCTCACGGCCCTGCTGTCGGTCGCCGTGGCCGCCACCCTCACTCCCGGCTGGG
GAACCCTGGCGGCCCTCGTGCTGACCTGGGTACTGGTGGCGCTCACCTTCATCGATCTGGACAAGATGCTGCTGCCGGAC
CAGCTCACCCTGCCACTGCTGTGGGGCGGCCTGCTGTTCAACCTGCCGGGAGGCTTCGTCCCCCTGGTGGATGCGGTGAT
CGGCGCCATGGCAGGCTATCTGGTGCTCTGGAGCCTCTACTGGGCCTTCAAGCTGCTGACCGGCAAGGAAGGGATGGGCT
ATGGCGACTTCAAACTGCTGGCGGCGCTGGGCGCCTGGCTCGGCTGGCAAGCCTTGCCCATCGTGCTGCTGCTTTCCTCG
CTGGCAGGCGCCATCATCGGCATCGGTCTCATCCTGCTGCGCAATCACCACCAGGGCAAACCCATTCCCTTTGGCCCCTA
TCTCGCCATCGCGGGCTGGATAACGCTGCTGTGGGGCGATAGCATCACCCGCTGGTATCTTGATGTCGTGCTCTAG

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(139-248)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

61.538

98.282

0.605
  pilD Vibrio campbellii strain DS40M4

57.241

99.656

0.57
  pilD Acinetobacter baumannii D1279779

54.122

95.876

0.519
  pilD Acinetobacter nosocomialis M2

54.122

95.876

0.519
  pilD Neisseria gonorrhoeae MS11

50.923

93.127

0.474