Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JC622_RS20905 Genome accession   NZ_CP066125
Coordinates   4466450..4468156 (+) Length   568 a.a.
NCBI ID   WP_227095387.1    Uniprot ID   -
Organism   Aeromonas veronii strain 32286     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4461450..4473156
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JC622_RS20880 pdhR 4462605..4463369 (-) 765 WP_043762834.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  JC622_RS20885 ampD 4463646..4464218 (-) 573 WP_227095384.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  JC622_RS20890 - 4464330..4464800 (+) 471 WP_227095385.1 TIGR02281 family clan AA aspartic protease -
  JC622_RS20895 nadC 4464804..4465679 (+) 876 WP_227095386.1 carboxylating nicotinate-nucleotide diphosphorylase -
  JC622_RS20900 - 4466012..4466443 (+) 432 WP_227096674.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  JC622_RS20905 pilB 4466450..4468156 (+) 1707 WP_227095387.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  JC622_RS20910 pilC 4468393..4469634 (+) 1242 WP_005336136.1 type II secretion system F family protein Machinery gene
  JC622_RS20915 pilD 4469695..4470567 (+) 873 WP_227095388.1 A24 family peptidase Machinery gene
  JC622_RS20920 coaE 4470581..4471195 (+) 615 WP_227095389.1 dephospho-CoA kinase -
  JC622_RS20925 zapD 4471233..4471955 (+) 723 WP_005355104.1 cell division protein ZapD -
  JC622_RS20930 yacG 4471965..4472159 (+) 195 WP_201908670.1 DNA gyrase inhibitor YacG -
  JC622_RS20935 mutT 4472237..4472641 (-) 405 WP_227095390.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62603.97 Da        Isoelectric Point: 5.7428

>NTDB_id=517622 JC622_RS20905 WP_227095387.1 4466450..4468156(+) (pilB) [Aeromonas veronii strain 32286]
MTSSPNSGLALSLAASSLISEEDSQRYLSQARAQRKPFVTFLIENEILDSKALADFCKLEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNIRNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=517622 JC622_RS20905 WP_227095387.1 4466450..4468156(+) (pilB) [Aeromonas veronii strain 32286]
ATGACCTCTAGCCCTAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAGGGCCCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGATTTTTGCAAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCACGTCCTGCCCATCTACACCCAGGGGCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGGCTTCATACCGAGGCGCTGCTAGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGTGAAAACGACGCCCTCGGCATGGAGGATATCGACGAGTCCGAGATT
TCTGATCTCGAGGTCTCCGACGAGAACAGCCGGCTGGACGAAAGCGTCAATACCGCCGATGACGATGCCCCCATCGTCAA
GTACATCAACAAGATCCTGATGGATGCCATCAAGCGCGGCGCCTCCGACCTGCACTTCGAGCCCTATGAGTTCAAGTATC
GGGTACGCTTTCGCATCGATGGCATTCTGCATGAAATTGCTACGCCGCCGGTCAACCTCTCCAACCGCTTCTCCGCTCGC
CTCAAGGTGATGGCGCGACTCGATATTGCCGAGCGGCGGTTGCCGCAGGATGGCCGCATCAAGTTGAAGTTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTTAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAGCCACAG
GGAATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCGCAACACCAC
CGAGGTCAATATCTCTACCGCCGAAGATCCGGTGGAGATCAATCTGCCCGGCGTCAACCAGGTGCAGGTCAACCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCTTGCGGCAAGATCCGGATGTGGTGATGGTGGGTGAAATTCGCGAT
CTCGAAACCGCCGAAATCGCCATCAAGGCAGCACAGACCGGCCACCTGGTGCTCTCCACCCTGCATACCAACTCCGCCGC
CGAGACCCTGACCAGGATGATGAACATGGGGGTTCCGGCTTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGCTGGCCCGTAAACTGTGCGACCACTGCAAGGCACCGGAAGTTGTGCCGGAAGCAGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAGCTGGCTGCCGGACTGCGACTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATATACGAAATCATGCTGATGTCGGAGAACATCGCCAAATTGATTATGCAGGGGGCAAACTCCCTGCAAATCGCCG
CTATCGCGCAGAAGGAGGGGATGCGCACCCTGCGCACCTCGGGGCTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAGGTCAACAGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Acinetobacter baumannii D1279779

55.634

100

0.556
  pilB Legionella pneumophila strain ERS1305867

56.458

95.423

0.539
  pilB Vibrio cholerae strain A1552

54.578

98.063

0.535
  pilB Vibrio campbellii strain DS40M4

50.871

100

0.514
  pilB Vibrio parahaemolyticus RIMD 2210633

51.241

99.296

0.509
  pilF Neisseria gonorrhoeae MS11

46.631

99.296

0.463
  pilF Thermus thermophilus HB27

39.602

97.359

0.386
  pilB/pilB1 Synechocystis sp. PCC 6803

39.209

97.887

0.384