Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JC624_RS17665 Genome accession   NZ_CP066124
Coordinates   3681286..3682998 (+) Length   570 a.a.
NCBI ID   WP_068565291.1    Uniprot ID   -
Organism   Acinetobacter pittii strain 32292     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3676286..3687998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JC624_RS17630 nusA 3677174..3678658 (-) 1485 WP_002115134.1 transcription termination factor NusA -
  JC624_RS17635 rimP 3678696..3679220 (-) 525 WP_002114922.1 ribosome maturation factor RimP -
  JC624_RS17655 secG 3679858..3680187 (-) 330 WP_002115012.1 preprotein translocase subunit SecG -
  JC624_RS17660 tpiA 3680200..3680994 (-) 795 WP_068565290.1 triose-phosphate isomerase -
  JC624_RS17665 pilB 3681286..3682998 (+) 1713 WP_068565291.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JC624_RS17670 pilC 3683025..3684251 (+) 1227 WP_005079343.1 type II secretion system F family protein Machinery gene
  JC624_RS19500 pilD 3684251..3685111 (+) 861 WP_267461696.1 prepilin peptidase Machinery gene
  JC624_RS17685 coaE 3685113..3685709 (+) 597 WP_068565303.1 dephospho-CoA kinase -
  JC624_RS17690 - 3685706..3686620 (-) 915 WP_068565305.1 DMT family transporter -
  JC624_RS17695 rlmB 3686656..3687405 (-) 750 WP_005079348.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  JC624_RS17700 - 3687508..3687834 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63529.90 Da        Isoelectric Point: 5.0294

>NTDB_id=517591 JC624_RS17665 WP_068565291.1 3681286..3682998(+) (pilB) [Acinetobacter pittii strain 32292]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVNLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLVLKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILAQQFTEESSFEFSDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKTPADIPKQSLLEMGF
TEQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=517591 JC624_RS17665 WP_068565291.1 3681286..3682998(+) (pilB) [Acinetobacter pittii strain 32292]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAGAGAGCTAAACGGGATACAGTTGCGTACCTGATTGAAGAGGTTAATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATACAAGCCAAATT
CCCAAAGATTTGGTCGATCAGAAACTGGTTTTAAAACATCGGGTCTTACCTCTCATACAAAGAGGACAGATTTTATATGT
CGCAACCAGCAACCCGAGTAATATTGAAGCGATTGATGCCATTCGCTTTAATAGTAAGCTCATGGTTGAACCAGTCATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGGCGCAGCAATTTACCGAAGAAAGCAGCTTCGAATTTAGTGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCTCCACCCTCACAAGAGGATGAGGAAGAAACGCCTCAAGGTGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCAATTCGTATGGGGGCTTCAGACTTACATTTTGAACCCTATGAGAAATCAT
ATCGGGTCCGTTATCGAGTCGACGGCGTGTTACGTCAGATTGCAAATCCACCTTTGCAATTGGCTAACCGTTTGGCTTCA
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAGCGGGTACCTCAAGATGGCCGTATCAAGCTGAAGTTATCAAA
AACTAAAGCAATCGATTTTCGTGTGAACTCACTTCCAACGTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CTAGCGCGATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTCGATAAACCA
CAAGGTATGCTTTTAATTACAGGTCCTACCGGTTCTGGTAAAACGGTATCTTTATATACTGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGATCCAGTCGAAATCAACCTTGAAGGCATTAATCAGGTTAACGTTAACCCAA
AAGTAGGTCTTACTTTTTCGGCCGCACTGAAATCATTTTTACGTCAAGACCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTAGAAACGGCTGAAATTGCGATTAAAGCTGCACAAACCGGTCACATGGTAATGTCCACACTGCATACCAATAGTGC
GCCAGAAACACTTACTCGCCTACGTAATATGGGTGTTCCATCCTTTAATATCGCAACGTCTGTTAATCTGGTGATTGCTC
AACGTTTGGCGCGTCGGTTATGCCCACAGTGTAAAACACCTGCCGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGAACAAGATCTGACTCATCCCGATTTTCAAGTTTTCCAGCCTGTCGGTTGTACTGAGTGTCGTGAAGGCTACAAAGG
TCGAGTAGGTATTTACGAGGTGATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTCAATAATCTGCGTCGTTCAGGGTTAAAGAAAGTCATGCAAGGTGTAACTTCC
TTACAAGAAGTTAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.912

100

0.949
  pilB Acinetobacter baylyi ADP1

78.07

100

0.781
  pilB Legionella pneumophila strain ERS1305867

51.499

99.474

0.512
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

51.718

91.93

0.475
  pilB Vibrio campbellii strain DS40M4

50.766

91.579

0.465
  pilB Vibrio cholerae strain A1552

50.096

91.053

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.505

100

0.382
  pilF Thermus thermophilus HB27

36.833

98.596

0.363