Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I9190_RS01930 Genome accession   NZ_CP066121
Coordinates   421228..422940 (-) Length   570 a.a.
NCBI ID   WP_035300190.1    Uniprot ID   A0A8I1AKW3
Organism   Acinetobacter bereziniae strain GD0320     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 416228..427940
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9190_RS01900 (I9190_01900) - 416232..416555 (+) 324 WP_004718357.1 pyrimidine/purine nucleoside phosphorylase -
  I9190_RS01905 (I9190_01905) rlmB 416699..417451 (+) 753 WP_151780914.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  I9190_RS01910 (I9190_01910) - 417492..418412 (+) 921 WP_151780913.1 DMT family transporter -
  I9190_RS01915 (I9190_01915) coaE 418459..419058 (-) 600 WP_151780912.1 dephospho-CoA kinase -
  I9190_RS01920 (I9190_01920) pilD 419084..419965 (-) 882 WP_171496493.1 prepilin peptidase Machinery gene
  I9190_RS01925 (I9190_01925) pilC 419968..421200 (-) 1233 WP_151780911.1 type II secretion system F family protein Machinery gene
  I9190_RS01930 (I9190_01930) pilB 421228..422940 (-) 1713 WP_035300190.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I9190_RS01935 (I9190_01935) tpiA 423219..424013 (+) 795 WP_151780909.1 triose-phosphate isomerase -
  I9190_RS01940 (I9190_01940) secG 424023..424364 (+) 342 WP_004826367.1 preprotein translocase subunit SecG -
  I9190_RS01965 (I9190_01965) rimP 425158..425682 (+) 525 WP_004826368.1 ribosome maturation factor RimP -
  I9190_RS01970 (I9190_01970) nusA 425717..427201 (+) 1485 WP_151780908.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63142.64 Da        Isoelectric Point: 4.9084

>NTDB_id=517509 I9190_RS01930 WP_035300190.1 421228..422940(-) (pilB) [Acinetobacter bereziniae strain GD0320]
MSMQNGSPKFSGYIRRLVDEGFLSAQQMKDALTSAQKSKTHLVPYLIEKLAISPLLIAEMISSEFGEPIFDLDSFNPEML
PKDIVDAGLISKHGVIPLVQRGNILYVATSDPTNIEALDAIRFSCGLNIEPIIVEQHKLDKALAKHFTVEESFNFGDEDF
DVDIIATDENEPAELDEETAEEEAPIVKYVNKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRTITNPPLQLANRLAS
RLKVMSQMDISEKRIPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFMEALEKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNLKAGLTFSVALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCNHCKAPIEIPKQSLLELGF
TEQDLNHPDLQIFQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKLGFDNLRRSGLKKVMNGLTS
LQEINRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=517509 I9190_RS01930 WP_035300190.1 421228..422940(-) (pilB) [Acinetobacter bereziniae strain GD0320]
ATGTCTATGCAAAATGGTTCACCTAAGTTTTCGGGTTATATCCGTCGCTTAGTCGATGAAGGCTTTTTATCTGCTCAGCA
AATGAAAGATGCCTTAACAAGTGCTCAAAAAAGTAAAACCCATTTGGTGCCTTACCTGATTGAGAAATTAGCGATCTCAC
CATTGCTGATCGCTGAAATGATCTCCTCCGAGTTCGGTGAGCCTATTTTTGATCTAGACAGTTTTAATCCTGAAATGTTG
CCCAAGGACATTGTGGACGCCGGTTTAATCAGCAAACATGGGGTGATTCCATTGGTGCAACGCGGCAATATCTTATATGT
TGCCACCAGCGATCCCACCAATATCGAGGCACTTGATGCGATTCGTTTTAGTTGTGGTCTAAACATTGAGCCAATTATCG
TTGAACAACATAAATTAGACAAAGCTTTAGCCAAACATTTTACTGTAGAAGAAAGCTTTAATTTTGGCGATGAAGATTTT
GATGTTGATATTATTGCAACAGATGAAAATGAACCCGCCGAGCTTGATGAAGAAACTGCTGAAGAAGAAGCGCCGATTGT
TAAATATGTCAATAAACTATTGATTGATGCCATTCGAATGGGCGCCTCGGACTTACATTTTGAACCTTATGAAAAAAGTT
ATCGTGTTCGTTATCGTGTAGATGGTGTACTCAGAACTATCACCAATCCCCCTCTGCAATTGGCGAATCGTTTAGCATCC
CGTTTGAAAGTCATGTCTCAAATGGATATTTCAGAGAAACGTATTCCTCAAGATGGGCGGATCAAACTAAAACTGTCTAA
AACCAAAGCTATTGATTTCCGTGTCAACTCCCTGCCAACATTGTTTGGTGAAAAATTAGTTTTACGTATTCTTGATCCTT
CCAGTGCCATGTTAGGGATTGATGCTTTGGGCTATGAAGAAGACCAAAAAGCTTTATTTATGGAAGCCTTAGAAAAACCG
CAAGGCATGTTATTGATTACTGGTCCAACAGGTTCTGGTAAAACCGTATCGTTGTATACAGGCTTAAATATCCTCAATAC
CGAAAGTTCCAATATTTCAACCGCAGAAGACCCTGTTGAGATTAACTTGGAGGGGATTAACCAAGTTAACGTCAATCTTA
AAGCAGGATTGACCTTTTCAGTCGCGCTAAAATCATTTCTTCGTCAAGATCCAGATATTATCATGGTCGGGGAGATTCGA
GACCTTGAAACGGCTGAAATTGCAATCAAAGCAGCCCAAACAGGTCATATGGTGATGTCCACACTGCATACCAACAGTGC
ACCTGAAACCTTAACCCGTTTGCGCAATATGGGCGTGCCATCCTTTAATATTGCAACATCGGTCAATCTTGTGATTGCAC
AACGTCTTGCTCGACGTTTATGCAACCACTGTAAAGCACCTATTGAAATTCCTAAACAAAGTTTGTTAGAACTGGGCTTT
ACTGAACAAGACTTAAACCATCCTGATTTACAGATTTTTCAGCCTGTGGGCTGTTCTGAATGTAGAGAAGGTTATAAAGG
ACGAGTCGGTATTTATGAGGTGATGAAAGTAACACCTGAAATTTCAAAAATTATCATGGAAGATGGCAACGCACTGCAAA
TTGCAGCAGCATCTGAAAAATTGGGGTTTGATAATTTGCGACGTTCGGGTTTAAAAAAGGTTATGAATGGTTTAACGTCT
TTACAAGAAATTAACCGCGTAACCAGCGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.245

100

0.805
  pilB Acinetobacter baylyi ADP1

75.088

100

0.751
  pilB Legionella pneumophila strain ERS1305867

51.215

100

0.518
  pilF Neisseria gonorrhoeae MS11

49.644

98.596

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

48.654

97.719

0.475
  pilB Vibrio cholerae strain A1552

48.282

97.018

0.468
  pilB Vibrio campbellii strain DS40M4

49.712

91.404

0.454
  pilB/pilB1 Synechocystis sp. PCC 6803

34.91

100

0.375
  pilF Thermus thermophilus HB27

37.217

100

0.375