Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   THIVI_RS03900 Genome accession   NC_018012
Coordinates   867776..868294 (-) Length   172 a.a.
NCBI ID   WP_014777334.1    Uniprot ID   I3Y775
Organism   Thiocystis violascens DSM 198     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 862776..873294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THIVI_RS03890 (Thivi_0797) - 865233..867089 (+) 1857 WP_083845694.1 sulfotransferase -
  THIVI_RS03895 (Thivi_0798) - 867115..867441 (-) 327 WP_014777333.1 hypothetical protein -
  THIVI_RS03900 (Thivi_0799) pilE 867776..868294 (-) 519 WP_014777334.1 pilin Machinery gene
  THIVI_RS03905 (Thivi_0800) - 868779..869255 (+) 477 WP_014777335.1 SEL1-like repeat protein -
  THIVI_RS03910 (Thivi_0801) - 869793..870998 (-) 1206 Protein_796 IS3 family transposase -
  THIVI_RS03920 - 871077..871259 (+) 183 WP_041446814.1 hypothetical protein -
  THIVI_RS03930 (Thivi_0804) mazG 871693..872505 (+) 813 WP_014777337.1 nucleoside triphosphate pyrophosphohydrolase -

Sequence

Protein


Download         Length: 172 a.a.        Molecular weight: 16977.54 Da        Isoelectric Point: 7.7831

>NTDB_id=51645 THIVI_RS03900 WP_014777334.1 867776..868294(-) (pilE) [Thiocystis violascens DSM 198]
MKKFQQGFTLIELMIVVAIIGILAAIALPAYQDYIVRSKVSEAIIAASSAKSSASEAFMSDGVTGLAAASAAWGLIPAAE
KATKFVADVTMAAGGTVTVTTAGTAASGLPTAAAGMTLVFSPNVQNAAPAAGVTGGIDWACGSTTVATAGTRGLTNANTG
TMPAKYAPSECK

Nucleotide


Download         Length: 519 bp        

>NTDB_id=51645 THIVI_RS03900 WP_014777334.1 867776..868294(-) (pilE) [Thiocystis violascens DSM 198]
ATGAAAAAGTTCCAACAAGGCTTTACCCTGATCGAACTCATGATCGTCGTGGCGATCATTGGTATTTTGGCGGCTATCGC
CTTGCCGGCTTATCAAGACTACATCGTGCGTTCCAAGGTTTCCGAGGCCATTATCGCGGCATCCTCGGCTAAGTCCTCCG
CCAGCGAAGCATTTATGAGCGACGGCGTGACGGGCCTCGCTGCCGCATCGGCGGCTTGGGGTCTCATCCCCGCGGCTGAA
AAAGCAACCAAATTCGTCGCGGATGTGACCATGGCCGCTGGCGGTACGGTGACCGTCACCACGGCGGGTACGGCCGCTTC
CGGGTTGCCGACCGCTGCGGCTGGTATGACGCTTGTTTTCTCGCCAAACGTCCAAAACGCCGCTCCCGCCGCTGGCGTGA
CTGGTGGGATCGACTGGGCCTGCGGTTCAACCACCGTAGCAACAGCCGGTACACGCGGCTTGACTAACGCCAACACTGGT
ACCATGCCCGCCAAGTATGCTCCGTCCGAGTGCAAATAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-172)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I3Y775

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

53.723

100

0.587
  pilA Ralstonia pseudosolanacearum GMI1000

42.857

100

0.436
  comP Acinetobacter baylyi ADP1

40.571

100

0.413
  pilA2 Legionella pneumophila str. Paris

41.667

97.674

0.407
  pilA2 Legionella pneumophila strain ERS1305867

40.476

97.674

0.395
  pilE Neisseria gonorrhoeae MS11

38.15

100

0.384
  pilE Neisseria gonorrhoeae strain FA1090

36.628

100

0.366

Multiple sequence alignment