NTDB

Overview

Name	comEA/celA/cilE	Type	Machinery gene
Locus tag	I6H77_RS00685	Genome accession	NZ_CP066021
Coordinates	144081..144731 (-)	Length	216 a.a.
NCBI ID	WP_000452010.1	Uniprot ID	A0A7H9FBM2
Organism	Streptococcus oralis strain FDAARGOS_1020
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 139081..149731

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6H77_RS00665 (I6H77_00665)	rplT	140463..140822 (-)	360	WP_000124830.1	50S ribosomal protein L20	-
I6H77_RS00670 (I6H77_00670)	rpmI	140874..141074 (-)	201	WP_001125942.1	50S ribosomal protein L35	-
I6H77_RS00675 (I6H77_00675)	infC	141107..141637 (-)	531	WP_000848184.1	translation initiation factor IF-3	-
I6H77_RS00680 (I6H77_00680)	comEC/celB	141857..144097 (-)	2241	WP_000083028.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
I6H77_RS00685 (I6H77_00685)	comEA/celA/cilE	144081..144731 (-)	651	WP_000452010.1	helix-hairpin-helix domain-containing protein	Machinery gene
I6H77_RS00690 (I6H77_00690)	-	144798..145367 (-)	570	WP_000443753.1	GNAT family N-acetyltransferase	-
I6H77_RS00695 (I6H77_00695)	ald	145544..146656 (+)	1113	WP_000904713.1	alanine dehydrogenase	-
I6H77_RS00700 (I6H77_00700)	-	146707..147693 (-)	987	WP_000658168.1	PhoH family protein	-
I6H77_RS00705 (I6H77_00705)	-	147774..147989 (-)	216	WP_001232091.1	YozE family protein	-
I6H77_RS00710 (I6H77_00710)	-	147986..148591 (-)	606	WP_000727655.1	GrpB family protein	-
I6H77_RS00715 (I6H77_00715)	cvfB	148603..149457 (-)	855	WP_001095468.1	RNA-binding virulence regulatory protein CvfB	-

Sequence

Protein

Download Length: 216 a.a. Molecular weight: 23183.41 Da Isoelectric Point: 5.9255

                                >NTDB_id=516433 I6H77_RS00685 WP_000452010.1 144081..144731(-) (comEA/celA/cilE) [Streptococcus oralis strain FDAARGOS_1020]

MEVIIEKIKEYKIIVICAVLGLALGAFFLLKPTSQTSVKETNLQAEVAAVSKDSSSEKEIKKEEKEESPEQDLITVDVKG

AVKSPGIYDLPVGSRVHDAVQKAGGLTDEADSKSLNLAQKVSDEALVYVPTKGEEAASQQAASGTTPSTSKEKKVNLNKA

SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTAD

Nucleotide

Download Length: 651 bp

                                >NTDB_id=516433 I6H77_RS00685 WP_000452010.1 144081..144731(-) (comEA/celA/cilE) [Streptococcus oralis strain FDAARGOS_1020]

ATGGAAGTAATTATCGAGAAAATCAAAGAGTATAAAATCATTGTCATCTGTGCTGTTTTAGGCTTGGCCTTGGGCGCATT

TTTCCTACTAAAGCCAACTTCACAAACATCTGTGAAAGAAACAAACTTGCAGGCTGAAGTTGCAGCTGTTTCAAAGGATT

CATCATCTGAAAAGGAAATCAAGAAGGAAGAAAAGGAAGAATCTCCTGAACAAGATCTAATCACAGTAGATGTTAAGGGG

GCTGTTAAATCGCCAGGGATTTATGATTTGCCAGTAGGGAGTCGTGTTCATGATGCCGTTCAGAAGGCAGGTGGCTTGAC

AGATGAGGCAGATAGCAAGTCGCTCAATCTAGCACAGAAAGTCAGTGACGAGGCTCTTGTCTATGTTCCAACTAAGGGAG

AAGAAGCAGCTAGTCAGCAGGCTGCCTCTGGAACGACTCCTTCGACAAGTAAAGAAAAGAAAGTCAACCTCAATAAAGCT

AGTCTGGAAGAACTAAAGCAGGTCAAAGGCTTGGGAGGAAAACGAGCCCAGGATATTATCGACCATCGTGAGGCAAATGG

CAAATTCAAGTCGGTTGATGAATTAAAGAAGGTCTCTGGTATTGGCGCTAAGACCATAGAAAAGCTAAAAGATTATGTCA

CAGCGGATTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(151-214)

SLBB domain (SLBB)

(76-126)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A7H9FBM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA/celA/cilE	Streptococcus mitis NCTC 12261	89.815	100	0.898
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	89.352	100	0.894
comEA/celA/cilE	Streptococcus pneumoniae D39	87.5	100	0.875
comEA/celA/cilE	Streptococcus pneumoniae R6	87.5	100	0.875
comEA/celA/cilE	Streptococcus pneumoniae Rx1	87.5	100	0.875
comEA/celA/cilE	Streptococcus mitis SK321	85.648	100	0.856
comEA	Lactococcus lactis subsp. cremoris KW2	41.485	100	0.44
comEA	Streptococcus thermophilus LMD-9	39.32	95.37	0.375

Detailed information NTG0516433