Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   I6I17_RS09180 Genome accession   NZ_CP065999
Coordinates   1808979..1810262 (-) Length   427 a.a.
NCBI ID   WP_003782394.1    Uniprot ID   F0EYR9
Organism   Kingella denitrificans strain FDAARGOS_1060     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1803979..1815262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6I17_RS09150 (I6I17_09150) rph 1804426..1805154 (-) 729 WP_040564714.1 ribonuclease PH -
  I6I17_RS09155 (I6I17_09155) - 1805405..1806025 (-) 621 WP_003782385.1 L-threonylcarbamoyladenylate synthase -
  I6I17_RS09160 (I6I17_09160) pncC 1806070..1806546 (-) 477 WP_040564260.1 nicotinamide-nucleotide amidase -
  I6I17_RS09165 (I6I17_09165) zapD 1806568..1807317 (-) 750 WP_003782389.1 cell division protein ZapD -
  I6I17_RS09170 (I6I17_09170) coaE 1807408..1808016 (-) 609 WP_003782391.1 dephospho-CoA kinase -
  I6I17_RS09175 (I6I17_09175) pilD 1808020..1808856 (-) 837 WP_040564261.1 A24 family peptidase Machinery gene
  I6I17_RS09180 (I6I17_09180) pilG 1808979..1810262 (-) 1284 WP_003782394.1 type II secretion system F family protein Machinery gene
  I6I17_RS09185 (I6I17_09185) pilF 1810306..1811985 (-) 1680 WP_115324816.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6I17_RS09190 (I6I17_09190) lysA 1812203..1813426 (-) 1224 WP_003782402.1 diaminopimelate decarboxylase -
  I6I17_RS09195 (I6I17_09195) lptM 1813413..1813598 (-) 186 WP_003782403.1 LPS translocon maturation chaperone LptM -
  I6I17_RS09200 (I6I17_09200) cyaY 1813684..1814004 (+) 321 WP_040564715.1 iron donor protein CyaY -
  I6I17_RS09205 (I6I17_09205) prfB 1814069..1815172 (-) 1104 WP_003782406.1 peptide chain release factor 2 -

Sequence

Protein


Download         Length: 427 a.a.        Molecular weight: 46681.08 Da        Isoelectric Point: 10.1640

>NTDB_id=516256 I6I17_RS09180 WP_003782394.1 1808979..1810262(-) (pilG) [Kingella denitrificans strain FDAARGOS_1060]
MAERKPARKVASKKAPVKKAARPEKKEKGNRYQFEGRNLSTDSIVRGEVVAKNEEDARQKLARRQIRVLQLNKMKSAREK
KITQQDITVFTRQLSTMMKAGLPLMQAFDIVAKGHSNAAVTRLLTEIRNDVEQGESLGTSFSKHPKYFDKFYCNLVAAGE
AGGVLEGLLDKLALYKEKTQAIKKKVKSALTYPIAIVVVAVVLVIVMMMFVLPEFGKVYSSMGAELPPLTQFMMNISNAF
VAYGWLIILGLIGGVVGLIKFHQSSPAFQKKVDSWLLKLPIFGAIVRKATLARWARTTATLFTAGVPLVEVLESVAGASG
NIIYEEATMVIRSKVNQGLSLTSGLQGAGTDMFPNMMVQMAAIGEESGALDDMLNKIAEFYEEEVDVAVASLSSLMEPVI
MVVLGVIIGTILVAMYLPLFNLGNVVA

Nucleotide


Download         Length: 1284 bp        

>NTDB_id=516256 I6I17_RS09180 WP_003782394.1 1808979..1810262(-) (pilG) [Kingella denitrificans strain FDAARGOS_1060]
ATGGCTGAAAGAAAACCAGCAAGAAAGGTGGCTTCTAAAAAAGCACCTGTGAAAAAAGCGGCAAGACCGGAGAAGAAAGA
AAAGGGTAACCGCTATCAGTTTGAAGGCCGCAATTTAAGCACGGATTCCATTGTTCGCGGCGAGGTGGTTGCCAAAAACG
AAGAGGATGCACGTCAAAAATTGGCGCGCCGTCAGATTCGTGTTTTGCAATTGAACAAAATGAAAAGCGCCCGTGAGAAA
AAAATCACGCAACAGGACATTACTGTGTTCACGCGCCAATTGTCTACCATGATGAAAGCTGGCCTTCCGTTGATGCAGGC
ATTCGATATTGTGGCGAAAGGCCATAGTAATGCTGCTGTTACCCGATTGCTGACGGAAATCCGCAATGATGTGGAACAAG
GTGAATCTTTAGGCACATCGTTTTCCAAACACCCAAAATATTTCGATAAGTTCTATTGCAATTTGGTTGCCGCAGGTGAA
GCAGGTGGTGTATTAGAAGGACTGTTGGATAAGCTGGCACTTTATAAAGAAAAAACCCAAGCCATCAAGAAAAAAGTAAA
ATCGGCGTTGACTTACCCGATTGCCATTGTGGTGGTGGCCGTGGTTTTGGTCATTGTGATGATGATGTTCGTACTGCCGG
AATTTGGTAAGGTATACAGCAGTATGGGCGCTGAATTGCCACCATTGACACAGTTTATGATGAATATCTCTAACGCATTT
GTGGCTTATGGTTGGTTGATTATTCTTGGCCTGATCGGTGGTGTGGTTGGTTTGATTAAATTCCACCAAAGCTCTCCGGC
ATTCCAGAAAAAAGTCGATTCTTGGTTGTTGAAATTACCTATTTTCGGAGCCATTGTACGGAAAGCAACGCTGGCACGCT
GGGCGCGGACCACAGCAACTCTGTTTACGGCAGGTGTGCCATTGGTGGAAGTATTGGAATCTGTGGCAGGTGCTTCGGGT
AATATTATTTACGAAGAGGCCACGATGGTCATCCGTTCCAAAGTGAATCAAGGTCTTTCTTTGACTTCAGGCTTGCAAGG
TGCCGGTACTGATATGTTCCCCAATATGATGGTGCAGATGGCGGCCATTGGTGAGGAATCGGGTGCACTGGACGATATGC
TGAATAAAATCGCCGAGTTTTATGAAGAAGAAGTGGATGTTGCGGTAGCGAGCTTGTCTTCTTTGATGGAACCGGTCATC
ATGGTGGTACTGGGCGTTATCATTGGTACCATCTTGGTGGCGATGTACTTGCCGTTGTTCAATTTGGGTAACGTAGTCGC
TTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(90-213)

Type II secretion system (T2SS), protein F (T2SSF)

(294-418)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F0EYR9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

65.509

94.379

0.618
  pilG Neisseria meningitidis 44/76-A

64.516

94.379

0.609
  pilC Legionella pneumophila strain ERS1305867

47.5

93.677

0.445
  pilC Acinetobacter baumannii D1279779

45.22

90.632

0.41
  pilC Pseudomonas stutzeri DSM 10701

46.257

87.588

0.405
  pilC Acinetobacter baylyi ADP1

43.928

90.632

0.398