Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   I6I17_RS09185 Genome accession   NZ_CP065999
Coordinates   1810306..1811985 (-) Length   559 a.a.
NCBI ID   WP_115324816.1    Uniprot ID   -
Organism   Kingella denitrificans strain FDAARGOS_1060     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1805306..1816985
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6I17_RS09155 (I6I17_09155) - 1805405..1806025 (-) 621 WP_003782385.1 L-threonylcarbamoyladenylate synthase -
  I6I17_RS09160 (I6I17_09160) pncC 1806070..1806546 (-) 477 WP_040564260.1 nicotinamide-nucleotide amidase -
  I6I17_RS09165 (I6I17_09165) zapD 1806568..1807317 (-) 750 WP_003782389.1 cell division protein ZapD -
  I6I17_RS09170 (I6I17_09170) coaE 1807408..1808016 (-) 609 WP_003782391.1 dephospho-CoA kinase -
  I6I17_RS09175 (I6I17_09175) pilD 1808020..1808856 (-) 837 WP_040564261.1 A24 family peptidase Machinery gene
  I6I17_RS09180 (I6I17_09180) pilG 1808979..1810262 (-) 1284 WP_003782394.1 type II secretion system F family protein Machinery gene
  I6I17_RS09185 (I6I17_09185) pilF 1810306..1811985 (-) 1680 WP_115324816.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6I17_RS09190 (I6I17_09190) lysA 1812203..1813426 (-) 1224 WP_003782402.1 diaminopimelate decarboxylase -
  I6I17_RS09195 (I6I17_09195) lptM 1813413..1813598 (-) 186 WP_003782403.1 LPS translocon maturation chaperone LptM -
  I6I17_RS09200 (I6I17_09200) cyaY 1813684..1814004 (+) 321 WP_040564715.1 iron donor protein CyaY -
  I6I17_RS09205 (I6I17_09205) prfB 1814069..1815172 (-) 1104 WP_003782406.1 peptide chain release factor 2 -
  I6I17_RS09210 (I6I17_09210) - 1815271..1815726 (-) 456 WP_003782407.1 HAD domain-containing protein -
  I6I17_RS09215 (I6I17_09215) rlmH 1815740..1816210 (-) 471 WP_003782409.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62155.99 Da        Isoelectric Point: 5.4569

>NTDB_id=516257 I6I17_RS09185 WP_115324816.1 1810306..1811985(-) (pilF) [Kingella denitrificans strain FDAARGOS_1060]
MSVGLLRVLVQKKLVPQENLAKYQEAIKKEKPILPLVFSDKIITPDALAQLCSDVFNYPMLDLNFFPRSRIVPDVLTEEQ
MQELRCIPLHKRGKKLYVAVSDPTNLQNIQKAVFSTGLSADLVVVRDDQLSTVLEWFGQSNTALLKEMGMDVQKEESAQT
MIVDGEEEEDGPVARFIQKILHDAVTSGASDIHFEFYEFMARVRFRTDGQLREIVQPPLALRSQLASRIKVMAKLDISEK
RVPQDGRIQLQFSKNTKAIDFRVNTMPCLFGEKVVMRILNSDAASLNIDQLGFEDFQKEMIMEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTDDVNISTAEDPAEINLPGINQVNVNDKQGLTFEAALKAFLRQDPDIIMIGEIRDLGTADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASAVNLIMAQRLARRLCSACKAPMERPPEQALRQAGFTDEDLAKDWTM
YRAVGCDACKGKGYKGRAGLYEVMPMTEKMKAVIMKGGTEVDIANIAYEEGLCDLRRSGLLKVMQGITTLEEVIATTNE

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=516257 I6I17_RS09185 WP_115324816.1 1810306..1811985(-) (pilF) [Kingella denitrificans strain FDAARGOS_1060]
ATGAGCGTCGGCTTATTGCGCGTATTGGTGCAGAAAAAATTAGTTCCACAAGAAAACCTCGCAAAATATCAAGAAGCTAT
TAAAAAAGAAAAACCGATTCTGCCCCTGGTGTTCAGCGACAAAATCATTACGCCGGATGCGTTGGCGCAACTGTGCTCCG
ACGTTTTCAACTATCCGATGCTGGATTTGAATTTTTTCCCGCGTTCGCGCATTGTTCCCGACGTATTGACCGAAGAGCAA
ATGCAGGAATTGCGCTGCATACCGCTGCATAAACGCGGTAAAAAACTGTATGTGGCCGTTTCCGATCCGACCAATCTGCA
AAATATCCAAAAGGCGGTTTTCAGTACCGGTTTGTCGGCCGATTTGGTTGTGGTACGTGATGATCAGTTAAGCACCGTTT
TGGAATGGTTTGGTCAAAGCAACACCGCTCTGTTGAAAGAAATGGGCATGGATGTGCAGAAAGAAGAGTCTGCGCAAACC
ATGATTGTGGATGGCGAAGAGGAAGAGGATGGTCCGGTAGCGCGTTTCATCCAAAAAATCCTGCATGATGCCGTCACTTC
CGGTGCATCCGATATTCACTTTGAGTTTTACGAATTTATGGCACGCGTGCGTTTCCGTACCGACGGCCAGTTGCGCGAAA
TCGTACAGCCGCCTTTGGCATTGCGCAGCCAGTTGGCATCGCGTATCAAGGTAATGGCGAAGCTGGATATTTCTGAAAAA
CGCGTACCTCAGGATGGACGCATCCAGTTGCAGTTTTCCAAAAATACCAAAGCCATCGACTTCCGTGTGAATACCATGCC
CTGCCTGTTTGGCGAAAAAGTCGTGATGCGTATTCTGAATTCCGATGCCGCCAGCTTGAATATCGACCAATTGGGTTTTG
AAGATTTCCAAAAAGAGATGATTATGGAGGCGATTCACCGTCCGTACGGCATGGTGTTGGTAACAGGACCTACCGGTTCG
GGTAAAACCGTATCGCTTTATACCTGCCTGAATATTCTGAACACGGACGATGTGAATATTTCGACTGCAGAAGACCCGGC
AGAGATTAACTTGCCCGGTATCAACCAGGTAAACGTGAACGATAAACAAGGCCTGACGTTTGAAGCGGCGCTGAAAGCAT
TCCTTCGACAAGACCCGGATATCATCATGATTGGTGAGATTCGCGACTTGGGCACTGCCGATATTGCGATTAAAGCTGCA
CAAACAGGCCACATGGTGTTTTCTACCTTGCACACGAACAATGCACCGGCTACATTGTCGCGTATGTTGAACATGGGTGT
GGCGCCGTTTAATATTGCATCGGCAGTTAATCTGATTATGGCGCAACGTTTGGCGCGCCGCTTGTGTTCAGCCTGTAAAG
CGCCAATGGAACGTCCGCCGGAGCAGGCATTGCGTCAAGCGGGCTTTACCGACGAGGACTTGGCGAAAGATTGGACCATG
TATCGCGCCGTAGGTTGCGATGCCTGCAAAGGTAAAGGCTATAAAGGGCGTGCAGGTTTGTATGAAGTTATGCCGATGAC
GGAAAAAATGAAAGCCGTTATTATGAAAGGCGGTACTGAGGTTGATATTGCGAATATCGCCTATGAAGAAGGATTGTGTG
ATTTGCGTCGTTCAGGCTTGTTGAAAGTCATGCAGGGCATTACGACGTTAGAGGAAGTGATTGCCACAACCAACGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

69.946

100

0.699
  pilB Acinetobacter baumannii D1279779

52.313

100

0.526
  pilB Acinetobacter baylyi ADP1

51.957

100

0.522
  pilB Legionella pneumophila strain ERS1305867

48.036

100

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

45.213

100

0.456
  pilB Vibrio cholerae strain A1552

45.887

97.853

0.449
  pilB Vibrio campbellii strain DS40M4

46.402

94.454

0.438
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.848

94.275

0.376
  pilF Thermus thermophilus HB27

38.563

94.633

0.365