Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   YSA_RS11325 Genome accession   NC_017986
Coordinates   2327694..2328956 (-) Length   420 a.a.
NCBI ID   WP_017148006.1    Uniprot ID   A0A7L9GK88
Organism   Pseudomonas putida ND6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2322694..2333956
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  YSA_RS11310 (YSA_04289) - 2324360..2325961 (-) 1602 WP_012053948.1 AAA family ATPase -
  YSA_RS11315 (YSA_04291) aroB 2325977..2327074 (-) 1098 WP_003249149.1 3-dehydroquinate synthase -
  YSA_RS11320 (YSA_04292) aroK 2327171..2327689 (-) 519 WP_010955625.1 shikimate kinase AroK -
  YSA_RS11325 (YSA_04297) pilQ 2327694..2328956 (-) 1263 WP_017148006.1 secretin N-terminal domain-containing protein Machinery gene
  YSA_RS11330 (YSA_04298) - 2328880..2329881 (-) 1002 WP_014754728.1 pilus assembly protein PilP -
  YSA_RS11335 (YSA_04299) - 2329893..2330429 (-) 537 WP_004575490.1 PilN domain-containing protein -
  YSA_RS11340 (YSA_04302) pilM 2330426..2331307 (-) 882 WP_017148007.1 pilus assembly protein PilM -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45716.39 Da        Isoelectric Point: 4.9839

>NTDB_id=51576 YSA_RS11325 WP_017148006.1 2327694..2328956(-) (pilQ) [Pseudomonas putida ND6]
MKTWMKYLLATLLSIPLASATPYQGEPLSLNFQDVEVRAVLQVLADYAGVNLVASDAVQGSVTLRLQDVPWDQALDLVLR
SKGLSRRQEGNVLLVAPAAEFAAQSADARVSQVLDAQLQPLRRELLTIHHAKAADLAQLLLASLEGGGISTGSLSVDERT
NTLVVYLPADRLAEMRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYGEKLRPGKELFVDLGLERAGSSIGL
GLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVSFREASLSLEVTPQITPDNRV
IMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEKKS
ELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=51576 YSA_RS11325 WP_017148006.1 2327694..2328956(-) (pilQ) [Pseudomonas putida ND6]
ATGAAAACGTGGATGAAATACCTGCTGGCAACCCTGCTGTCGATCCCGCTGGCTTCGGCGACGCCTTACCAGGGTGAACC
CCTGTCGCTGAACTTCCAGGATGTAGAGGTGCGTGCGGTGCTGCAGGTGCTGGCTGACTACGCCGGCGTCAACCTGGTTG
CCAGTGATGCGGTACAAGGCAGCGTCACTTTGCGTTTGCAGGACGTGCCGTGGGATCAGGCGCTCGACCTGGTGTTGCGC
AGCAAGGGCCTGTCCCGGCGCCAGGAGGGCAATGTGCTCTTGGTGGCGCCTGCGGCCGAGTTTGCCGCGCAATCGGCAGA
CGCCCGGGTGAGCCAAGTGCTGGACGCGCAGCTGCAGCCATTGCGTCGGGAGCTGCTGACCATTCACCACGCCAAGGCCG
CCGATTTGGCGCAGTTGCTGCTGGCAAGTCTGGAGGGTGGCGGTATCTCCACGGGTAGCCTGAGTGTCGATGAGCGCACC
AATACGCTGGTGGTGTACCTGCCCGCTGACCGCCTGGCCGAGATGCGCCAGTTGGTGGCGCAGTTGGACGTGCCGGTGCG
TCAGGTAGCGATCGAGGCGCGTATCGTCGAGGCCAACGTCGATTATGAGAAAAGCCTGGGGGTACGCTGGGGTGGGCCGC
TGTACGGCGAGAAACTGCGGCCCGGCAAGGAGCTGTTCGTCGACCTCGGTCTGGAACGAGCCGGCAGCAGTATCGGCCTG
GGTCTGTTGCGCGGTGACGTGCTGCTGGACCTGGAGCTCAGTGCCATGGAAAAAAGCGGCAACGGCGAGATCATTTCGCA
GCCCAAGGTGGTCACGGCTGACAAGGAAACGGCGAGGATCCTCAAGGGTACCGAGGTGCCTTACCAGGAAACGAGCCAAA
GCGGCGCTACCTCGGTATCGTTTCGCGAAGCCTCGCTGTCGCTCGAAGTAACACCACAGATCACTCCGGACAACAGGGTG
ATCATGACCGTCAGGGTGACCAAGGACGAGCCGGATTACGTCAATGCGTTGAACAACGTACCGCCGATCCGCAAGAACGA
GGTCAATGCCAAGGTTCGTGTGGCTGACGGCGAAACGATCGTGATCGGTGGCGTCTACTCGACTTCGCAAAACAATGTGG
TCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCC
GAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(255-411)

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(126-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7L9GK88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.633

100

0.619
  comQ Acinetobacter baylyi ADP1

42.667

100

0.457
  pilQ Acinetobacter baumannii strain A118

40.781

100

0.448
  pilQ Acinetobacter baumannii D1279779

40.781

100

0.448
  pilQ Legionella pneumophila strain ERS1305867

43.029

99.048

0.426
  pilQ Legionella pneumophila strain Lp02

43.029

99.048

0.426
  pilQ Vibrio campbellii strain DS40M4

41.244

100

0.426
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.651

98.81

0.421
  pilQ Vibrio cholerae strain A1552

42.651

98.81

0.421

Multiple sequence alignment