Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6G33_RS10600 Genome accession   NZ_CP065721
Coordinates   2232959..2234662 (-) Length   567 a.a.
NCBI ID   WP_102827579.1    Uniprot ID   -
Organism   Stutzerimonas degradans strain FDAARGOS_876     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2227959..2239662
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G33_RS10565 (I6G33_10565) rpsI 2227986..2228378 (-) 393 WP_009867980.1 30S ribosomal protein S9 -
  I6G33_RS10570 (I6G33_10570) rplM 2228393..2228821 (-) 429 WP_009867981.1 50S ribosomal protein L13 -
  I6G33_RS10575 (I6G33_10575) - 2229234..2229917 (+) 684 WP_102827574.1 energy-coupling factor ABC transporter permease -
  I6G33_RS10580 (I6G33_10580) yacG 2229963..2230160 (-) 198 WP_102827575.1 DNA gyrase inhibitor YacG -
  I6G33_RS10585 (I6G33_10585) coaE 2230157..2230762 (-) 606 WP_102827576.1 dephospho-CoA kinase -
  I6G33_RS10590 (I6G33_10590) pilD 2230867..2231736 (-) 870 WP_102827577.1 A24 family peptidase Machinery gene
  I6G33_RS10595 (I6G33_10595) pilC 2231740..2232957 (-) 1218 WP_102827578.1 type II secretion system F family protein Machinery gene
  I6G33_RS10600 (I6G33_10600) pilB 2232959..2234662 (-) 1704 WP_102827579.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6G33_RS10605 (I6G33_10605) pilE 2234984..2235508 (+) 525 WP_003302811.1 pilin Machinery gene
  I6G33_RS10610 (I6G33_10610) tfpZ 2235669..2236403 (+) 735 WP_102827580.1 TfpX/TfpZ family type IV pilin accessory protein -
  I6G33_RS10615 (I6G33_10615) cysN 2236714..2238612 (-) 1899 WP_102827581.1 sulfate adenylyltransferase subunit CysN -
  I6G33_RS10620 (I6G33_10620) cysD 2238625..2239542 (-) 918 WP_008567291.1 sulfate adenylyltransferase subunit CysD -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62116.35 Da        Isoelectric Point: 5.6172

>NTDB_id=513494 I6G33_RS10600 WP_102827579.1 2232959..2234662(-) (pilB) [Stutzerimonas degradans strain FDAARGOS_876]
MSDNISLSGLARQMVLAGVLDEKTAQQAQIQALRSKASLVSYVVQNKLAKGRMVAELASEQFGVALLDLSAVDKETMPKE
LVSEKLARQHRLLPLARRGNKLYVAVSDPANHQAITDVQFSTGLHIEAILVEDDKLGQALDKFYESDATGLEDLGDVDLD
GLDVETVADDVKGDEAGGDADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRLRTDGILHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKISKTKAIDFRVNTLPTLWGEKIVMRILDPSSARMGIDALGYEEAQKELYMNALRQPQ
GMILVTGPTGSGKTVSLYTGLNILNTIDVNISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPSCKKEIDVPRDTLLDEGFP
VDRLGTFKIYAPVGCDNCKGGYKGRVGIYEVVKITPALQRIIMEDGNSIDIATQMRKDGFNDLRTSALAKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=513494 I6G33_RS10600 WP_102827579.1 2232959..2234662(-) (pilB) [Stutzerimonas degradans strain FDAARGOS_876]
ATGAGCGACAATATTTCCCTTTCGGGGCTGGCCCGGCAGATGGTGCTGGCCGGCGTTCTGGATGAAAAGACCGCCCAGCA
GGCGCAGATCCAGGCGTTGCGCAGCAAGGCGTCGCTGGTCAGCTATGTAGTGCAGAATAAGCTGGCGAAAGGGCGAATGG
TGGCCGAGCTGGCGTCTGAGCAGTTCGGTGTCGCGCTGTTGGATCTGTCGGCGGTGGACAAGGAAACCATGCCGAAGGAG
CTGGTCAGCGAGAAACTGGCGCGCCAGCATCGGCTGCTGCCGCTGGCGCGGCGTGGCAACAAGCTCTATGTCGCGGTGTC
GGATCCGGCCAACCATCAGGCCATTACCGATGTCCAGTTCAGTACCGGGCTGCATATCGAGGCCATCCTGGTCGAGGATG
ACAAGCTCGGCCAGGCGCTGGACAAGTTCTATGAAAGCGATGCGACCGGGCTGGAAGATCTGGGCGACGTCGACCTGGAT
GGCCTCGATGTCGAAACCGTCGCCGATGACGTCAAAGGAGACGAGGCGGGCGGCGACGCCGATGATGCGCCGGTGGTCCG
CTTCGTCAACAAGATGCTGCTCGATGCGATTCGCGGCGGTTCGTCGGACCTGCACTTTGAGCCCTATGAAAAGGCCTACC
GGGTCCGATTGCGTACCGATGGCATCCTGCATGAGGTGGCGCGGCCGCCGATCCAGCTCGCGCCGCGCATCTCGGCACGT
CTCAAGGTGATGGCTGGGCTGGATATCTCCGAGCGACGCAAGCCGCAGGATGGCCGCATCAAGATGAAGATATCCAAAAC
CAAGGCCATCGACTTTCGGGTCAACACGCTGCCGACGCTGTGGGGGGAGAAGATCGTCATGCGGATTCTCGATCCATCCA
GTGCGCGGATGGGCATCGATGCGCTGGGTTATGAGGAAGCGCAGAAGGAGCTGTACATGAATGCCTTGCGCCAGCCGCAG
GGCATGATCCTGGTGACCGGGCCGACCGGTTCCGGCAAGACGGTTTCGCTGTATACCGGCCTGAACATCCTCAATACCAT
CGATGTGAATATCTCGACGGCCGAAGACCCGGTGGAGATCAACTTGGAGGGCATCAACCAGGTCAACGTCAACCCTCGCC
AGGGTATGGACTTCGCCCAGGCGCTGCGTGCGTTCCTGCGCCAGGACCCGGACATCATCATGGTTGGCGAGATTCGCGAC
CTGGAAACCGCTGAGATCGCCATCAAGGCGGCGCAGACCGGGCACATGGTGATGTCGACGCTGCACACCAACAGCGCTGC
TGAGACGCTGACGCGCCTGCGCAACATGGGCGTGCCGGCGTTCAACATTGCGACTTCGGTGAACCTGATTATCGCGCAAC
GTTTGGCGCGCAAGCTTTGTCCGAGCTGCAAGAAGGAAATCGACGTGCCGCGCGATACGCTGCTGGACGAGGGCTTCCCT
GTCGATCGGCTCGGTACGTTCAAAATATACGCCCCGGTGGGTTGCGATAACTGCAAGGGGGGCTACAAGGGGCGTGTCGG
TATTTATGAAGTGGTTAAAATCACGCCTGCATTGCAGCGCATTATCATGGAAGACGGCAATTCAATCGATATAGCCACCC
AGATGCGTAAAGACGGTTTCAATGATCTGCGAACTTCGGCACTTGCGAAGGCCATGCAGGGCGTTACCAGTCTGGAAGAA
GTCAACCGGGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.929

99.471

0.596
  pilB Acinetobacter baylyi ADP1

59.752

99.471

0.594
  pilB Legionella pneumophila strain ERS1305867

55.732

100

0.557
  pilF Neisseria gonorrhoeae MS11

49.381

99.647

0.492
  pilB Vibrio cholerae strain A1552

51.103

95.944

0.49
  pilB Vibrio campbellii strain DS40M4

48.85

99.647

0.487
  pilB Vibrio parahaemolyticus RIMD 2210633

52.5

91.711

0.481
  pilB/pilB1 Synechocystis sp. PCC 6803

36.364

100

0.395