Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   I6G43_RS01765 Genome accession   NZ_CP065706
Coordinates   363966..364982 (+) Length   338 a.a.
NCBI ID   WP_068981970.1    Uniprot ID   -
Organism   Streptococcus oralis strain FDAARGOS_886     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 358966..369982
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G43_RS01745 (I6G43_01745) nagA 360539..361690 (+) 1152 WP_001134442.1 N-acetylglucosamine-6-phosphate deacetylase -
  I6G43_RS01750 (I6G43_01750) tnpA 361832..362347 (-) 516 Protein_329 IS200/IS605 family transposase -
  I6G43_RS01755 (I6G43_01755) - 362646..363011 (+) 366 WP_038805747.1 DUF1033 family protein -
  I6G43_RS01760 (I6G43_01760) comGA/cglA/cilD 363077..364018 (+) 942 WP_038805745.1 competence type IV pilus ATPase ComGA Machinery gene
  I6G43_RS01765 (I6G43_01765) comGB/cglB 363966..364982 (+) 1017 WP_068981970.1 competence type IV pilus assembly protein ComGB Machinery gene
  I6G43_RS01770 (I6G43_01770) comGC/cglC 364984..365307 (+) 324 WP_038805744.1 competence type IV pilus major pilin ComGC Machinery gene
  I6G43_RS01775 (I6G43_01775) comGD/cglD 365270..365704 (+) 435 WP_038805743.1 competence type IV pilus minor pilin ComGD Machinery gene
  I6G43_RS01780 (I6G43_01780) comGE/cglE 365667..365969 (+) 303 WP_000413373.1 competence type IV pilus minor pilin ComGE Machinery gene
  I6G43_RS01785 (I6G43_01785) comGF/cglF 365932..366393 (+) 462 WP_000531552.1 competence type IV pilus minor pilin ComGF Machinery gene
  I6G43_RS01790 (I6G43_01790) comGG/cglG 366371..366796 (+) 426 WP_000265654.1 competence type IV pilus minor pilin ComGG Machinery gene
  I6G43_RS01795 (I6G43_01795) - 366806..367396 (+) 591 WP_000679771.1 class I SAM-dependent methyltransferase -
  I6G43_RS01800 (I6G43_01800) comYH 367455..368408 (+) 954 WP_001008566.1 class I SAM-dependent methyltransferase Machinery gene
  I6G43_RS01805 (I6G43_01805) - 368458..369648 (+) 1191 WP_000167782.1 acetate kinase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38567.71 Da        Isoelectric Point: 9.6718

>NTDB_id=513191 I6G43_RS01765 WP_068981970.1 363966..364982(+) (comGB/cglB) [Streptococcus oralis strain FDAARGOS_886]
MDISQVFRLKRKKLATAKQKKIITLFHNLFSSGFHLVEIISFLGRSALLEKDYVAQMHQGLSQGKSFSEMMESLGFSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLSKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LALVLGCSLILLLALTFYKRSSKMRVFSILARFPFLGIFVQTYLTAYYAREWGNMISQGMELTQIFQIMKEQGSQLFKEI
GQDLAQALQNGREFSQTIATYPFFKKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=513191 I6G43_RS01765 WP_068981970.1 363966..364982(+) (comGB/cglB) [Streptococcus oralis strain FDAARGOS_886]
ATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTGGCTACAGCTAAGCAAAAGAAAATCATCACCCTGTTTCA
CAATCTCTTCTCCAGTGGTTTTCATTTGGTGGAAATTATTTCTTTCTTGGGTAGAAGTGCCCTGCTGGAAAAGGACTATG
TAGCCCAGATGCACCAAGGCTTGTCTCAGGGGAAATCCTTCTCAGAAATGATGGAAAGTTTGGGCTTTTCAAGTGCTATT
GTGACTCAATTATCTCTAGCTGAAGTTCATGGAAATCTCCATCTGAGTCTGGGGAAGATAGAAGAATATCTGGATAATTT
GTCCAAGGTCAAGAAGAAGTTAATTGAAGTAGCGACTTATCCCCTGATTTTGCTGGGATTTCTCCTGCTAATCATGTTGG
GGCTCAGGAATTATTTGCTCCCCCAACTGGACAGTAGCAATATCGCCACTCAAATCATCGGCAATCTGCCACAAATTTTT
CTGGCACTAGTGCTGGGTTGTTCTCTAATTCTACTTTTAGCCCTCACTTTCTACAAAAGAAGTTCAAAAATGCGCGTCTT
TTCGATATTGGCACGGTTTCCCTTTCTGGGAATCTTTGTCCAGACCTATCTGACGGCCTATTACGCGCGTGAATGGGGCA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATCATGAAGGAACAAGGTTCCCAACTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAGCCCTACAAAATGGCCGTGAATTTTCTCAAACCATAGCGACCTATCCCTTCTTTAAAAAGGA
GTTGAGTCTCATCATTGAGTATGGGGAAGTCAAATCCAAGCTGGGGAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATCTGGTGCAGCCACTGGTTTTTATCTTTGTGGCCCTAATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis SK321

93.195

100

0.932
  comGB/cglB Streptococcus mitis NCTC 12261

92.308

100

0.923
  comGB/cglB Streptococcus pneumoniae Rx1

90.828

100

0.908
  comGB/cglB Streptococcus pneumoniae D39

90.828

100

0.908
  comGB/cglB Streptococcus pneumoniae R6

90.828

100

0.908
  comGB/cglB Streptococcus pneumoniae TIGR4

90.828

100

0.908
  comYB Streptococcus gordonii str. Challis substr. CH1

72.917

99.408

0.725
  comYB Streptococcus mutans UA140

58.934

94.379

0.556
  comYB Streptococcus mutans UA159

58.934

94.379

0.556
  comGB Lactococcus lactis subsp. cremoris KW2

52.679

99.408

0.524