Detailed information NTG0513195

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: I6G43_RS01785
• Genome accession: NZ_CP065706
• Coordinates: 365932..366393 (+)
• Length: 153 a.a.
• NCBI ID: WP_000531552.1
• Uniprot ID: A0A4V0BPL9
• Organism: Streptococcus oralis strain FDAARGOS_886
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 360932..371393

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6G43_RS01750 (I6G43_01750)	tnpA	361832..362347 (-)	516	Protein_329	IS200/IS605 family transposase	-
I6G43_RS01755 (I6G43_01755)	-	362646..363011 (+)	366	WP_038805747.1	DUF1033 family protein	-
I6G43_RS01760 (I6G43_01760)	comGA/cglA/cilD	363077..364018 (+)	942	WP_038805745.1	competence type IV pilus ATPase ComGA	Machinery gene
I6G43_RS01765 (I6G43_01765)	comGB/cglB	363966..364982 (+)	1017	WP_068981970.1	competence type IV pilus assembly protein ComGB	Machinery gene
I6G43_RS01770 (I6G43_01770)	comGC/cglC	364984..365307 (+)	324	WP_038805744.1	competence type IV pilus major pilin ComGC	Machinery gene
I6G43_RS01775 (I6G43_01775)	comGD/cglD	365270..365704 (+)	435	WP_038805743.1	competence type IV pilus minor pilin ComGD	Machinery gene
I6G43_RS01780 (I6G43_01780)	comGE/cglE	365667..365969 (+)	303	WP_000413373.1	competence type IV pilus minor pilin ComGE	Machinery gene
I6G43_RS01785 (I6G43_01785)	comGF/cglF	365932..366393 (+)	462	WP_000531552.1	competence type IV pilus minor pilin ComGF	Machinery gene
I6G43_RS01790 (I6G43_01790)	comGG/cglG	366371..366796 (+)	426	WP_000265654.1	competence type IV pilus minor pilin ComGG	Machinery gene
I6G43_RS01795 (I6G43_01795)	-	366806..367396 (+)	591	WP_000679771.1	class I SAM-dependent methyltransferase	-
I6G43_RS01800 (I6G43_01800)	comYH	367455..368408 (+)	954	WP_001008566.1	class I SAM-dependent methyltransferase	Machinery gene
I6G43_RS01805 (I6G43_01805)	-	368458..369648 (+)	1191	WP_000167782.1	acetate kinase	-
I6G43_RS01810 (I6G43_01810)	rnpA	369808..370179 (+)	372	WP_000748125.1	ribonuclease P protein component	-
I6G43_RS01815 (I6G43_01815)	-	370154..370966 (+)	813	WP_000728102.1	YidC/Oxa1 family membrane protein insertase	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17907.67 Da
• Isoelectric Point: 9.8000

>NTDB_id=513195 I6G43_RS01785 WP_000531552.1 365932..366393(+) (comGF/cglF) [Streptococcus oralis strain FDAARGOS_886]
MGQRKCWILKSSKVRAFTLLESLLALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELERSQFKKVEDN
HLYLKQDGKDIAMGKSKSDDFRKTDSSGRGYQPMVYGLKSAQITEENQLIRFRFQFQKGLEREFIYRVEKAKS

Nucleotide

• Length: 462 bp

>NTDB_id=513195 I6G43_RS01785 WP_000531552.1 365932..366393(+) (comGF/cglF) [Streptococcus oralis strain FDAARGOS_886]
ATGGGTCAGAGAAAATGTTGGATCTTAAAGAGCAGTAAGGTAAGAGCTTTTACTCTTTTAGAATCTCTGCTTGCCCTTAT
CGTCATTAGCGGTGGTTTGCTTCTCTTTCAAGCCATGAGTCAGCTCCTTATTTCAGAAGTGCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTATTTGTGGACCAGCTAGAGGCTGAGTTAGAGCGTTCGCAGTTTAAAAAGGTGGAGGACAAT
CACCTCTATCTGAAACAAGACGGCAAGGATATCGCTATGGGTAAGTCCAAATCAGATGATTTTCGGAAAACCGATAGCAG
TGGTCGAGGTTACCAGCCAATGGTTTATGGCCTCAAATCAGCTCAGATCACAGAGGAAAATCAACTGATTCGTTTTCGTT
TCCAATTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGCAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

3D structure

• AlphaFold DB: A0A4V0BPL9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus mitis NCTC 12261	85.621	100	0.856
comGF/cglF	Streptococcus mitis SK321	85.621	100	0.856
comGF/cglF	Streptococcus pneumoniae D39	84.967	100	0.85
comGF/cglF	Streptococcus pneumoniae TIGR4	84.967	100	0.85
comGF/cglF	Streptococcus pneumoniae R6	84.967	100	0.85
comGF/cglF	Streptococcus pneumoniae Rx1	84.967	100	0.85
comGF	Lactococcus lactis subsp. cremoris KW2	41.429	91.503	0.379
comYF	Streptococcus mutans UA159	42.222	88.235	0.373
comYF	Streptococcus mutans UA140	42.222	88.235	0.373