Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   I6G67_RS15310 Genome accession   NZ_CP065666
Coordinates   3079300..3081012 (+) Length   570 a.a.
NCBI ID   WP_004692712.1    Uniprot ID   A0A380UA98
Organism   Acinetobacter johnsonii strain FDAARGOS_910     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3074300..3086012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G67_RS15265 (I6G67_15265) nusA 3074879..3076363 (-) 1485 WP_004692706.1 transcription termination factor NusA -
  I6G67_RS15270 (I6G67_15270) rimP 3076403..3076927 (-) 525 WP_004692708.1 ribosome maturation factor RimP -
  I6G67_RS15300 (I6G67_15300) secG 3077886..3078215 (-) 330 WP_004692710.1 preprotein translocase subunit SecG -
  I6G67_RS15305 (I6G67_15305) tpiA 3078229..3079020 (-) 792 WP_004692711.1 triose-phosphate isomerase -
  I6G67_RS15310 (I6G67_15310) pilB 3079300..3081012 (+) 1713 WP_004692712.1 type IV-A pilus assembly ATPase PilB Machinery gene
  I6G67_RS15315 (I6G67_15315) pilC 3081043..3082269 (+) 1227 WP_004692713.1 type II secretion system F family protein Machinery gene
  I6G67_RS15320 (I6G67_15320) pilD 3082269..3083129 (+) 861 WP_004692714.1 A24 family peptidase Machinery gene
  I6G67_RS15325 (I6G67_15325) coaE 3083136..3083735 (+) 600 WP_004692715.1 dephospho-CoA kinase -
  I6G67_RS15330 (I6G67_15330) - 3083749..3084666 (-) 918 WP_004692716.1 DMT family transporter -
  I6G67_RS15335 (I6G67_15335) rlmB 3084699..3085448 (-) 750 WP_004692717.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  I6G67_RS15340 (I6G67_15340) - 3085591..3085914 (-) 324 WP_004692718.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63578.01 Da        Isoelectric Point: 5.1455

>NTDB_id=512798 I6G67_RS15310 WP_004692712.1 3079300..3081012(+) (pilB) [Acinetobacter johnsonii strain FDAARGOS_910]
MSVSNGLPKFTGFIRRLVEDGRMTAENMQLAMLSAKKAQQDIVPYLIENHKLSPQMIAETISLEFGEPVFDLSVYDSVQI
LRDLVEDKLITKHRVLPIYRRGNILYIATSNPTNMDAMDAIRFNSKMNIEAIIVEHPQLEKLIEQNFAQADSFEFSDEDI
DLDLEQDHQNSNDKEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKDLFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAAVDIPEQSLLEMGF
TSTDIKDPDFKVYQPVGCAECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=512798 I6G67_RS15310 WP_004692712.1 3079300..3081012(+) (pilB) [Acinetobacter johnsonii strain FDAARGOS_910]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGACGCATGACTGCTGAAAA
TATGCAGCTGGCAATGCTCTCTGCAAAAAAAGCTCAGCAAGACATCGTTCCTTATCTGATTGAAAATCATAAACTCTCCC
CACAAATGATCGCTGAAACGATTTCTTTAGAGTTTGGCGAGCCTGTTTTTGACTTAAGTGTCTATGATTCTGTACAAATT
CTACGGGATCTGGTCGAAGACAAACTTATCACCAAGCATCGTGTGTTGCCGATTTACCGTCGTGGCAATATCCTCTATAT
CGCCACTAGCAATCCAACCAACATGGATGCGATGGACGCGATTCGTTTCAATTCTAAAATGAACATTGAAGCGATTATTG
TTGAACATCCTCAACTTGAAAAACTAATCGAACAAAATTTCGCGCAAGCCGATAGCTTTGAATTTTCTGATGAAGATATT
GATTTAGACTTAGAGCAAGACCATCAGAACTCAAATGACAAAGAAGATGACTCACCTCAAGGGGATGAAGCTCCGATCGT
CAAATACATCAATAAATTACTGATCGATGCGATTCGTATGGGCGCCTCAGACTTACACTTTGAGCCTTATGAAAAATCTT
ACCGTGTTCGTTACCGTGTAGATGGAGTTCTGCGTCATATTGCCAGTCCGCCATTACAACTGGCGACTCGTCTTGCCTCA
CGTTTAAAAGTTATGTCACAAATGGACATCTCTGAAAAGCGCGTCCCGCAAGATGGCCGAATTAAATTAAAGCTGTCGAA
AAGCAAAGCCATCGACTTCCGTGTCAACTCACTTCCAACCTTATTTGGCGAAAAGCTGGTTCTACGTATTCTCGATCCAT
CCAGTGCTATGCTGGGGATTGATGCTTTAGGTTATGAACCAGATCAAAAAGACCTATTTATGCAGGCTTTAGACAAACCG
CAAGGCATGTTATTAATTACTGGCCCGACAGGTTCAGGTAAAACGGTATCGCTGTATACAGGTTTGAATATTCTAAATAA
AGAAGACACCAATATTTCAACTGCGGAAGACCCTGTTGAGATTAACTTAGAAGGCATTAATCAGGTCAATGTGAACAATA
AAGTCGGCCTCACCTTCTCTGCTGCGCTGAAGTCCTTCTTACGTCAGGATCCTGATATTGTAATGGTGGGTGAGATTCGT
GACCTTGAAACGGCTGAAATCGCGATTAAAGCCGCACAGACAGGTCACATGGTCATGTCGACACTCCACACCAATAGTGC
ACCTGAAACCCTGACCCGCTTACGTAATATGGGGGTTCCATCTTTTAACATTGCGACCTCGGTCAATTTGGTGATTGCAC
AGCGTTTGGCACGCCGCTTATGCAGCCAATGCAAAGCCGCTGTCGATATTCCTGAACAAAGCTTGCTCGAAATGGGCTTT
ACTTCCACAGATATCAAAGATCCAGACTTCAAAGTTTATCAACCGGTGGGGTGTGCTGAGTGCCGTGAAGGCTATAAAGG
ACGTGTTGGTATTTATGAAGTCATGAAAGTCACGCCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCTTACAGA
TTGCGGAAGCATCTGCAAAATTAGGATTTAACAATTTACGTCGTTCAGGTTTACTCAAGGTTATGCAGGGGGTCACTTCC
TTACAAGAAGTGAATCGTGTAACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A380UA98

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.228

100

0.812
  pilB Acinetobacter baylyi ADP1

76.491

100

0.765
  pilB Legionella pneumophila strain ERS1305867

53.283

93.509

0.498
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio cholerae strain A1552

50.566

92.982

0.47
  pilB Vibrio parahaemolyticus RIMD 2210633

50.677

90.702

0.46
  pilB Vibrio campbellii strain DS40M4

49.338

92.807

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

34.628

100

0.375
  pilF Thermus thermophilus HB27

36.427

100

0.365