Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   I6G86_RS02405 Genome accession   NZ_CP065637
Coordinates   416763..417257 (+) Length   164 a.a.
NCBI ID   WP_003133131.1    Uniprot ID   A0A1I4HNK8
Organism   Lactococcus garvieae strain FDAARGOS_929     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 364170..418940 416763..417257 within 0


Gene organization within MGE regions


Location: 364170..418940
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G86_RS02110 (I6G86_02110) - 365456..365815 (+) 360 WP_332871907.1 ArsC/Spx/MgsR family protein -
  I6G86_RS02115 (I6G86_02115) - 365977..366141 (+) 165 WP_169740176.1 hypothetical protein -
  I6G86_RS02120 (I6G86_02120) - 366385..366681 (+) 297 WP_042219880.1 BRCT domain-containing protein -
  I6G86_RS02125 (I6G86_02125) - 366856..367461 (+) 606 WP_042219918.1 recombinase family protein -
  I6G86_RS02130 (I6G86_02130) - 367513..367887 (-) 375 WP_042219915.1 ArsC/Spx/MgsR family protein -
  I6G86_RS10765 - 368092..368193 (-) 102 WP_255204113.1 putative holin-like toxin -
  I6G86_RS02135 (I6G86_02135) - 368373..371342 (-) 2970 WP_197911534.1 BspA family leucine-rich repeat surface protein -
  I6G86_RS02140 (I6G86_02140) - 371768..372103 (-) 336 WP_162174787.1 ArsC/Spx/MgsR family protein -
  I6G86_RS02145 (I6G86_02145) - 372168..372362 (-) 195 WP_042219940.1 hypothetical protein -
  I6G86_RS02150 (I6G86_02150) - 372368..372796 (-) 429 WP_232254523.1 polysaccharide deacetylase family protein -
  I6G86_RS02155 (I6G86_02155) - 372851..373741 (-) 891 WP_042219927.1 IS982-like element ISLll1 family transposase -
  I6G86_RS02160 (I6G86_02160) - 373818..374135 (-) 318 WP_150385311.1 polysaccharide deacetylase family protein -
  I6G86_RS02165 (I6G86_02165) - 374301..375455 (-) 1155 Protein_368 IS3 family transposase -
  I6G86_RS02170 (I6G86_02170) - 375726..376238 (+) 513 WP_042219033.1 hypothetical protein -
  I6G86_RS02175 (I6G86_02175) - 376235..376453 (+) 219 WP_019291529.1 hypothetical protein -
  I6G86_RS02180 (I6G86_02180) - 376578..376793 (+) 216 WP_042219031.1 hypothetical protein -
  I6G86_RS02185 (I6G86_02185) - 376837..377973 (+) 1137 WP_042219030.1 site-specific integrase -
  I6G86_RS02190 (I6G86_02190) recG 378110..380116 (+) 2007 WP_042219028.1 ATP-dependent DNA helicase RecG -
  I6G86_RS02195 (I6G86_02195) - 380153..380407 (+) 255 WP_042219026.1 hypothetical protein -
  I6G86_RS02200 (I6G86_02200) mnmE 380446..381810 (-) 1365 WP_003133081.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  I6G86_RS02205 (I6G86_02205) - 381941..382651 (+) 711 WP_014025528.1 pseudouridine synthase -
  I6G86_RS02210 (I6G86_02210) - 382654..383379 (+) 726 WP_023889716.1 ABC transporter ATP-binding protein -
  I6G86_RS02215 (I6G86_02215) - 383369..384460 (+) 1092 WP_042219024.1 ABC transporter permease -
  I6G86_RS02220 (I6G86_02220) rpiA 384537..385208 (+) 672 WP_042219022.1 ribose-5-phosphate isomerase RpiA -
  I6G86_RS02225 (I6G86_02225) - 385233..385685 (-) 453 WP_042219020.1 NUDIX domain-containing protein -
  I6G86_RS02230 (I6G86_02230) mreC 385805..386662 (+) 858 WP_042219018.1 rod shape-determining protein MreC -
  I6G86_RS02235 (I6G86_02235) mreD 386665..387171 (+) 507 WP_003133093.1 rod shape-determining protein MreD -
  I6G86_RS02240 (I6G86_02240) - 387313..388698 (+) 1386 WP_042219016.1 CHAP domain-containing protein -
  I6G86_RS02245 (I6G86_02245) - 388878..390080 (+) 1203 WP_042219014.1 CHAP domain-containing protein -
  I6G86_RS02250 (I6G86_02250) dnaJ 390292..391422 (+) 1131 WP_003133099.1 molecular chaperone DnaJ -
  I6G86_RS02255 (I6G86_02255) - 391494..392021 (+) 528 WP_042219012.1 YqeG family HAD IIIA-type phosphatase -
  I6G86_RS02260 (I6G86_02260) zwf 392195..393682 (-) 1488 WP_003133102.1 glucose-6-phosphate dehydrogenase -
  I6G86_RS02265 (I6G86_02265) - 393706..393885 (-) 180 WP_014025518.1 hypothetical protein -
  I6G86_RS02270 (I6G86_02270) - 393995..394840 (+) 846 WP_003133104.1 sulfite exporter TauE/SafE family protein -
  I6G86_RS02275 (I6G86_02275) - 394837..395205 (+) 369 WP_014025517.1 DUF1634 domain-containing protein -
  I6G86_RS02285 (I6G86_02285) - 395388..395594 (+) 207 WP_016171078.1 hypothetical protein -
  I6G86_RS02290 (I6G86_02290) - 395605..396489 (+) 885 WP_042219010.1 NAD(P)-dependent oxidoreductase -
  I6G86_RS02295 (I6G86_02295) argS 396529..398217 (-) 1689 WP_003133108.1 arginine--tRNA ligase -
  I6G86_RS02300 (I6G86_02300) argR 398592..399038 (+) 447 WP_003133109.1 arginine repressor -
  I6G86_RS02305 (I6G86_02305) - 399028..399369 (+) 342 WP_003133110.1 YlbF family regulator -
  I6G86_RS02310 (I6G86_02310) mutS 399478..401985 (+) 2508 WP_042219008.1 DNA mismatch repair protein MutS -
  I6G86_RS02315 (I6G86_02315) - 402051..402713 (+) 663 WP_042219006.1 hypothetical protein -
  I6G86_RS02320 (I6G86_02320) mutL 402886..404775 (+) 1890 WP_042219004.1 DNA mismatch repair endonuclease MutL -
  I6G86_RS02325 (I6G86_02325) - 404795..405757 (+) 963 WP_042219002.1 glycosyltransferase -
  I6G86_RS02330 (I6G86_02330) ruvA 405757..406347 (+) 591 WP_042219001.1 Holliday junction branch migration protein RuvA -
  I6G86_RS02335 (I6G86_02335) ruvB 406385..407383 (+) 999 WP_014025511.1 Holliday junction branch migration DNA helicase RuvB -
  I6G86_RS02340 (I6G86_02340) - 407480..407914 (+) 435 WP_042218999.1 low molecular weight protein-tyrosine-phosphatase -
  I6G86_RS02345 (I6G86_02345) - 408001..408366 (+) 366 WP_003133119.1 MerR family transcriptional regulator -
  I6G86_RS02350 (I6G86_02350) glnA 408423..409763 (+) 1341 WP_003133120.1 type I glutamate--ammonia ligase -
  I6G86_RS02355 (I6G86_02355) - 409828..410259 (+) 432 WP_042218996.1 hypothetical protein -
  I6G86_RS02360 (I6G86_02360) - 410252..410521 (-) 270 WP_042218995.1 chorismate mutase -
  I6G86_RS02365 (I6G86_02365) - 410661..410927 (+) 267 WP_042218993.1 DUF2089 family protein -
  I6G86_RS02370 (I6G86_02370) - 410929..411222 (+) 294 WP_042218991.1 hypothetical protein -
  I6G86_RS02375 (I6G86_02375) - 411279..411758 (+) 480 WP_014025504.1 GAF domain-containing protein -
  I6G86_RS02380 (I6G86_02380) - 411771..412391 (+) 621 WP_042218989.1 hypothetical protein -
  I6G86_RS02385 (I6G86_02385) dnaX 412404..414062 (+) 1659 WP_042218986.1 DNA polymerase III subunit gamma/tau -
  I6G86_RS02390 (I6G86_02390) - 414095..415558 (-) 1464 WP_003133128.1 amino acid permease -
  I6G86_RS02395 (I6G86_02395) - 416028..416240 (+) 213 WP_016171080.1 hypothetical protein -
  I6G86_RS02400 (I6G86_02400) rpsF 416432..416725 (+) 294 WP_003133129.1 30S ribosomal protein S6 -
  I6G86_RS02405 (I6G86_02405) ssb 416763..417257 (+) 495 WP_003133131.1 single-stranded DNA-binding protein Machinery gene
  I6G86_RS02410 (I6G86_02410) rpsR 417371..417616 (+) 246 WP_003133134.1 30S ribosomal protein S18 -
  I6G86_RS02415 (I6G86_02415) - 417732..418940 (+) 1209 WP_042218983.1 hypothetical protein -

Sequence


Protein


Download         Length: 164 a.a.        Molecular weight: 18003.88 Da        Isoelectric Point: 4.9533

>NTDB_id=512391 I6G86_RS02405 WP_003133131.1 416763..417257(+) (ssb) [Lactococcus garvieae strain FDAARGOS_929]
MINNVVLVGRVVRDPELRYTPQNTAVATFTIAVNRRFKNAQGEREADFINCVIWRQSAENLANWAKKGALIGVTGSIQVR
NYENKEGQRVYVTEVLADNFQMLESRAAREGGSGSYSAPQQQQSQNRPFAGSNSSAGNATPDFGRDADPFGGSPMEISDD
DLPF

Nucleotide


Download         Length: 495 bp        

>NTDB_id=512391 I6G86_RS02405 WP_003133131.1 416763..417257(+) (ssb) [Lactococcus garvieae strain FDAARGOS_929]
ATGATTAATAATGTTGTTTTGGTCGGACGCGTTGTGCGTGACCCTGAACTGCGATATACACCGCAAAATACTGCGGTTGC
TACATTTACGATTGCTGTTAACCGTCGTTTTAAAAACGCGCAAGGCGAACGTGAAGCTGATTTCATTAACTGTGTCATCT
GGCGTCAATCTGCTGAAAACTTGGCAAATTGGGCTAAAAAAGGTGCCTTGATTGGTGTAACAGGTAGTATTCAAGTGCGA
AATTATGAGAACAAAGAAGGTCAACGTGTTTACGTGACTGAAGTATTGGCTGATAACTTCCAAATGCTTGAGAGTCGTGC
CGCACGTGAAGGTGGCTCTGGTTCATATTCTGCACCACAGCAACAACAATCACAAAATCGTCCTTTTGCTGGCTCTAACA
GCTCAGCAGGAAATGCAACACCTGATTTTGGTCGTGATGCAGATCCATTTGGTGGCTCACCAATGGAAATCTCAGATGAT
GATCTTCCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A1I4HNK8

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

57.31

100

0.598

  ssbA Bacillus subtilis subsp. subtilis str. 168

53.933

100

0.585

  ssbB/cilA Streptococcus pneumoniae TIGR4

49.242

80.488

0.396

  ssbB/cilA Streptococcus pneumoniae D39

48.485

80.488

0.39

  ssbB/cilA Streptococcus pneumoniae R6

48.485

80.488

0.39

  ssbB/cilA Streptococcus mitis SK321

48.485

80.488

0.39

  ssbB/cilA Streptococcus mitis NCTC 12261

48.485

80.488

0.39

  ssbB/cilA Streptococcus pneumoniae Rx1

48.485

80.488

0.39

  ssbB Bacillus subtilis subsp. subtilis str. 168

57.547

64.634

0.372