Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q7A_RS03045 Genome accession   NC_017857
Coordinates   651934..653652 (-) Length   572 a.a.
NCBI ID   WP_014705867.1    Uniprot ID   I1XGH3
Organism   Methylophaga nitratireducenticrescens     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 646934..658652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7A_RS03010 (Q7A_639) - 647548..647733 (+) 186 WP_014705860.1 zinc-finger domain-containing protein -
  Q7A_RS03015 (Q7A_640) mutT 647737..648138 (+) 402 WP_014705861.1 8-oxo-dGTP diphosphatase MutT -
  Q7A_RS03020 (Q7A_641) yacG 648195..648380 (-) 186 WP_014705862.1 DNA gyrase inhibitor YacG -
  Q7A_RS03025 (Q7A_642) zapD 648383..649153 (-) 771 WP_014705863.1 cell division protein ZapD -
  Q7A_RS03030 (Q7A_643) coaE 649199..649807 (-) 609 WP_014705864.1 dephospho-CoA kinase -
  Q7A_RS03035 (Q7A_644) pilD 649809..650678 (-) 870 WP_014705865.1 A24 family peptidase Machinery gene
  Q7A_RS03040 (Q7A_645) pilC 650683..651930 (-) 1248 WP_014705866.1 type II secretion system F family protein Machinery gene
  Q7A_RS03045 (Q7A_646) pilB 651934..653652 (-) 1719 WP_014705867.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q7A_RS03050 (Q7A_647) lptM 653858..653986 (+) 129 WP_014705868.1 LPS translocon maturation chaperone LptM -
  Q7A_RS03055 (Q7A_648) lysA 653989..655242 (+) 1254 WP_014705869.1 diaminopimelate decarboxylase -
  Q7A_RS03060 (Q7A_649) dapF 655247..656095 (+) 849 WP_014705870.1 diaminopimelate epimerase -
  Q7A_RS03065 (Q7A_650) - 656071..656781 (+) 711 WP_014705871.1 DUF484 family protein -
  Q7A_RS03070 (Q7A_651) xerC 656783..657679 (+) 897 WP_407939581.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62942.17 Da        Isoelectric Point: 5.3152

>NTDB_id=51052 Q7A_RS03045 WP_014705867.1 651934..653652(-) (pilB) [Methylophaga nitratireducenticrescens]
MANMTAPIRLSGLARRLVSEGLLSEEQAHTAQVTAKQASQSFVTYLVNNKLLKSDVIGAIASQEFGVPLFDLDSMNMELA
PTSLFQEKLIRQHNALPLFQRGKRLYIAVSDPTNLQALDEFQFNTALNTFPVLVDEQKLSITIEKALDNKNQVLDDFDDA
ELDDIDLGPDDDAEAGAELTEKNIDDTPVVRFINGILTQAIKAGASDIHFEPYEKKYRVRVRIDGILHETKTAPVALGGR
IAARLKVLSRMNIAERRVPQDGRMRLKISKSKAIDFRVSTCPTLFGEKIVLRILDPTSAQLGIDALGYEEDQKKIFMETM
HKPYGMVLVTGPTGSGKTVSLYTALNILNTDDRNISTAEDPAEINLPGINQVNVHPQIGLGFTECLRAFLRQDPDVIMVG
EIRDLETAEIAIKAAQTGHMVFSTLHTNDAPQTLTRLANMGVPAFNIASAVTLIIAQRLARRLCQHCKKGETLPKETLIE
EGFSPEQIATDFKVYHAVGCEHCTEGYKGRVGIYQVMPVSEAMGRMIMEGGNSIQLADQAKAEGIDDLRASGLKKVMAGA
TSLEEINRVIKE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=51052 Q7A_RS03045 WP_014705867.1 651934..653652(-) (pilB) [Methylophaga nitratireducenticrescens]
ATGGCAAATATGACAGCACCAATTAGATTAAGCGGTCTTGCTAGGCGACTAGTCAGTGAAGGATTGCTGAGTGAAGAGCA
GGCACATACTGCTCAGGTCACCGCTAAACAGGCCTCACAATCTTTTGTGACCTATCTGGTCAATAATAAATTACTTAAAA
GTGATGTAATTGGTGCTATTGCTTCGCAGGAGTTTGGGGTTCCTCTGTTTGATCTTGATAGTATGAATATGGAGCTTGCC
CCTACCAGTCTTTTTCAGGAAAAACTGATTCGTCAGCATAATGCTTTACCGTTATTTCAACGGGGAAAACGCTTATATAT
AGCGGTATCCGATCCAACGAACCTGCAAGCACTGGACGAATTTCAGTTTAATACCGCGCTGAATACCTTTCCAGTTTTGG
TTGATGAGCAGAAATTATCCATCACTATTGAAAAGGCTCTGGATAATAAAAACCAGGTTTTAGATGATTTTGATGACGCG
GAACTGGATGATATTGATCTTGGGCCGGATGATGATGCCGAAGCTGGTGCTGAACTGACTGAAAAAAATATTGATGACAC
GCCTGTTGTCAGATTTATCAATGGCATTTTGACTCAAGCGATTAAAGCGGGTGCATCAGATATACACTTTGAACCTTATG
AGAAAAAATATCGAGTCAGAGTGCGTATTGACGGTATTTTACATGAAACAAAAACAGCTCCTGTCGCTTTAGGGGGAAGG
ATTGCCGCTCGTCTTAAAGTTTTATCACGTATGAATATTGCTGAACGTCGGGTACCTCAGGATGGCCGAATGCGGTTGAA
GATTTCCAAATCCAAAGCCATCGATTTTCGTGTCAGTACTTGCCCAACCTTGTTTGGTGAAAAAATCGTATTACGTATTC
TGGACCCAACCAGTGCCCAATTGGGGATCGATGCCCTGGGTTACGAGGAAGATCAGAAAAAAATCTTTATGGAAACCATG
CATAAACCCTATGGCATGGTGCTGGTCACCGGCCCGACGGGTAGTGGTAAAACCGTTTCTTTGTATACAGCACTGAATAT
TCTCAACACTGACGATCGTAATATCTCTACAGCAGAAGATCCTGCTGAAATTAATCTTCCCGGCATCAATCAGGTCAATG
TACATCCGCAGATTGGTTTGGGATTTACTGAATGCTTAAGAGCGTTCTTACGGCAGGATCCAGACGTTATCATGGTGGGT
GAGATCCGCGATCTGGAAACGGCTGAAATTGCCATTAAGGCTGCGCAGACTGGTCATATGGTGTTTTCAACATTGCATAC
CAACGATGCTCCCCAAACACTTACCCGTTTGGCCAATATGGGTGTGCCTGCGTTTAATATTGCCTCCGCCGTAACATTAA
TTATTGCGCAGCGTCTGGCACGTCGACTTTGTCAGCATTGTAAAAAGGGAGAAACATTACCAAAAGAAACCTTGATTGAA
GAAGGTTTCAGTCCTGAACAAATTGCGACAGATTTCAAAGTTTACCACGCTGTAGGTTGCGAACATTGCACCGAAGGGTA
TAAAGGTCGTGTTGGTATTTATCAAGTCATGCCCGTATCCGAAGCAATGGGCCGCATGATTATGGAAGGCGGAAATTCGA
TTCAACTTGCGGATCAGGCAAAAGCTGAAGGGATTGATGATTTGCGTGCATCCGGTCTGAAAAAGGTCATGGCCGGAGCG
ACTAGTTTGGAAGAAATTAACCGCGTCATTAAGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I1XGH3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.794

100

0.57
  pilB Acinetobacter baylyi ADP1

55.148

100

0.552
  pilB Legionella pneumophila strain ERS1305867

52.951

100

0.533
  pilB Vibrio parahaemolyticus RIMD 2210633

49.03

99.126

0.486
  pilB Vibrio cholerae strain A1552

50

96.503

0.483
  pilB Vibrio campbellii strain DS40M4

48.063

99.301

0.477
  pilF Neisseria gonorrhoeae MS11

48.387

97.552

0.472
  pilB/pilB1 Synechocystis sp. PCC 6803

36.748

100

0.395
  pilF Thermus thermophilus HB27

37.213

99.126

0.369

Multiple sequence alignment