Detailed information NTG0051050

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: Q7A_RS03035
• Genome accession: NC_017857
• Coordinates: 649809..650678 (-)
• Length: 289 a.a.
• NCBI ID: WP_014705865.1
• Uniprot ID: I1XGH1
• Organism: Methylophaga nitratireducenticrescens
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 644809..655678

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q7A_RS03005 (Q7A_638)	nadA	646418..647530 (+)	1113	WP_014705859.1	quinolinate synthase NadA	-
Q7A_RS03010 (Q7A_639)	-	647548..647733 (+)	186	WP_014705860.1	zinc-finger domain-containing protein	-
Q7A_RS03015 (Q7A_640)	mutT	647737..648138 (+)	402	WP_014705861.1	8-oxo-dGTP diphosphatase MutT	-
Q7A_RS03020 (Q7A_641)	yacG	648195..648380 (-)	186	WP_014705862.1	DNA gyrase inhibitor YacG	-
Q7A_RS03025 (Q7A_642)	zapD	648383..649153 (-)	771	WP_014705863.1	cell division protein ZapD	-
Q7A_RS03030 (Q7A_643)	coaE	649199..649807 (-)	609	WP_014705864.1	dephospho-CoA kinase	-
Q7A_RS03035 (Q7A_644)	pilD	649809..650678 (-)	870	WP_014705865.1	A24 family peptidase	Machinery gene
Q7A_RS03040 (Q7A_645)	pilC	650683..651930 (-)	1248	WP_014705866.1	type II secretion system F family protein	Machinery gene
Q7A_RS03045 (Q7A_646)	pilB	651934..653652 (-)	1719	WP_014705867.1	type IV-A pilus assembly ATPase PilB	Machinery gene
Q7A_RS03050 (Q7A_647)	lptM	653858..653986 (+)	129	WP_014705868.1	LPS translocon maturation chaperone LptM	-
Q7A_RS03055 (Q7A_648)	lysA	653989..655242 (+)	1254	WP_014705869.1	diaminopimelate decarboxylase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 31737.66 Da
• Isoelectric Point: 6.6829

>NTDB_id=51050 Q7A_RS03035 WP_014705865.1 649809..650678(-) (pilD) [Methylophaga nitratireducenticrescens]
MSLIYLLETSSIFFIASIFILGLLVGSFLNVVIHRLPLMMQREWAQQCHELIGTTEPETDELTLSKPRSRCPHCGHAITA
LENIPVLSYLALKGRCKECKAPISKRYPLIELFTAVISAIVAWHFGFDIAVVGALLLSWALLALTFIDVDHQLLPDSITL
PLLWLGLSFNLFAIYTDISSAVIGAIAGYLALWLVYHAFRLVTGKEGMGYGDFKLLAALGGWMGWQALPMIILLSSLVGA
LVGISLILLKQQHRDIPIPFGPYLAAAGWLALVWGDSLTTGYLNWSGLS

Nucleotide

• Length: 870 bp

>NTDB_id=51050 Q7A_RS03035 WP_014705865.1 649809..650678(-) (pilD) [Methylophaga nitratireducenticrescens]
ATGTCGCTAATTTATCTGCTGGAAACCTCCAGCATTTTTTTTATTGCCAGTATTTTCATTCTGGGATTACTGGTCGGCAG
CTTTCTCAATGTGGTGATTCACCGACTGCCGCTGATGATGCAGCGTGAGTGGGCCCAACAGTGCCATGAATTGATTGGAA
CGACTGAACCTGAGACAGATGAACTTACGTTAAGCAAACCGCGTTCTCGCTGCCCGCATTGTGGTCATGCGATTACTGCA
TTGGAAAATATTCCGGTACTCAGTTATCTCGCTCTGAAAGGTCGTTGCAAGGAATGCAAAGCGCCAATTTCCAAACGCTA
TCCGCTGATTGAATTATTCACTGCAGTGATCTCGGCGATAGTGGCATGGCACTTTGGTTTCGACATTGCCGTAGTGGGCG
CCTTATTGTTAAGTTGGGCTCTGCTGGCATTGACGTTTATCGATGTTGATCATCAACTGTTGCCCGACAGCATTACTTTA
CCCTTATTGTGGTTAGGACTCAGTTTTAACCTGTTTGCTATCTATACCGACATTAGCTCTGCCGTTATTGGTGCCATTGC
CGGTTATCTGGCGCTTTGGCTGGTGTATCACGCCTTTCGTTTGGTCACAGGTAAGGAAGGCATGGGCTATGGCGATTTTA
AGCTGCTGGCAGCCTTGGGAGGCTGGATGGGTTGGCAGGCGTTACCGATGATCATTTTATTATCCTCTTTAGTCGGTGCA
TTGGTTGGTATCAGCCTGATTTTGTTAAAACAACAGCATCGTGATATTCCCATTCCATTTGGTCCCTATCTGGCAGCAGC
CGGTTGGCTGGCGCTGGTCTGGGGCGACTCCCTGACCACCGGATACCTGAATTGGAGCGGATTAAGCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

3D structure

• AlphaFold DB: I1XGH1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	57.455	95.156	0.547
pilD	Vibrio campbellii strain DS40M4	53.455	95.156	0.509
pilD	Acinetobacter baumannii D1279779	52.313	97.232	0.509
pilD	Acinetobacter nosocomialis M2	52.313	97.232	0.509
pilD	Neisseria gonorrhoeae MS11	48.043	97.232	0.467