Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   Q7A_RS00295 Genome accession   NC_017857
Coordinates   63680..64738 (-) Length   352 a.a.
NCBI ID   WP_014705300.1    Uniprot ID   I1XEV5
Organism   Methylophaga nitratireducenticrescens     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 58680..69738
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7A_RS00270 (Q7A_57) aroK 59174..59701 (-) 528 WP_041354156.1 shikimate kinase AroK -
  Q7A_RS00275 (Q7A_59) pilQ 59794..61971 (-) 2178 WP_052501223.1 type IV pilus secretin PilQ -
  Q7A_RS00280 (Q7A_60) - 61982..62530 (-) 549 WP_014705297.1 pilus assembly protein PilP -
  Q7A_RS00285 (Q7A_61) - 62530..63117 (-) 588 WP_014705298.1 type 4a pilus biogenesis protein PilO -
  Q7A_RS00290 (Q7A_62) - 63120..63680 (-) 561 WP_014705299.1 PilN domain-containing protein -
  Q7A_RS00295 (Q7A_63) pilM 63680..64738 (-) 1059 WP_014705300.1 pilus assembly protein PilM Machinery gene
  Q7A_RS00300 (Q7A_64) - 64927..67326 (+) 2400 WP_041354158.1 penicillin-binding protein 1A -
  Q7A_RS15600 (Q7A_65) - 67474..68571 (-) 1098 WP_014705302.1 methyl-accepting chemotaxis protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38171.41 Da        Isoelectric Point: 4.8623

>NTDB_id=51047 Q7A_RS00295 WP_014705300.1 63680..64738(-) (pilM) [Methylophaga nitratireducenticrescens]
MFGRKKSPLLGIDISSSVVKLLELNKRGDRYTVESYAVEPLPVNAVVEGRIENSDEVAGAIKRAVKRSGTKSRDASVAVS
ANSAITKVVSFPSSISERDMEENVMLEAENYIPYPLDEVRLDFEVLGPSAADADAVDVLLAASRRENVDARTSVLEKAGL
KPRLVDVEAYTVENAFSLIARQLPNQGNGLTVAVADIGANLTTLHVLVNGKIVFTREQTFGGRMLTEDIERYYGMSYQEA
GRAKKDKSLPDDYEPKVLEPFKRSMAVTITRALQFFFSASSQYQSIDHIILAGGCASIEGVDKVVEVETGTSTSIANPFT
DMALGARVKVQALSNDAPSMMIACGLAMRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=51047 Q7A_RS00295 WP_014705300.1 63680..64738(-) (pilM) [Methylophaga nitratireducenticrescens]
ATGTTTGGACGTAAGAAATCCCCTTTACTAGGGATAGATATCAGCTCTTCAGTAGTAAAGCTGTTGGAGCTTAATAAGCG
CGGCGATCGTTATACGGTTGAATCCTATGCGGTGGAACCTCTTCCGGTAAATGCGGTGGTAGAAGGTCGCATCGAAAACA
GTGATGAAGTTGCTGGTGCGATAAAGCGTGCAGTGAAACGTTCTGGGACGAAATCTCGAGATGCTTCTGTCGCGGTTTCA
GCGAATTCAGCCATTACTAAAGTTGTTTCTTTTCCATCTTCCATTTCAGAACGTGATATGGAAGAAAATGTAATGTTGGA
AGCAGAAAATTATATTCCCTATCCACTTGATGAAGTCCGACTTGACTTTGAGGTTTTAGGTCCATCTGCCGCTGATGCCG
ATGCCGTAGATGTTTTATTAGCCGCTTCCCGCCGCGAAAATGTCGATGCTCGTACCAGTGTGCTGGAAAAAGCCGGACTC
AAACCGAGACTGGTCGATGTGGAAGCCTATACTGTCGAAAATGCTTTTTCTCTAATCGCCAGACAACTACCCAATCAAGG
TAATGGTTTAACCGTTGCTGTTGCAGATATCGGAGCTAATTTAACGACGTTACATGTTCTGGTAAACGGCAAAATTGTTT
TTACCAGAGAGCAGACTTTTGGCGGCCGAATGCTGACCGAAGACATTGAGCGCTATTATGGAATGTCTTATCAGGAAGCA
GGCCGTGCCAAAAAAGATAAGAGCTTGCCTGACGATTATGAACCAAAGGTACTGGAGCCTTTCAAACGTTCCATGGCAGT
AACCATCACTCGAGCCCTGCAGTTCTTCTTTTCAGCATCAAGCCAGTACCAATCAATTGATCATATTATCCTTGCTGGTG
GTTGTGCCTCGATTGAGGGAGTAGATAAGGTTGTTGAGGTAGAAACTGGTACCTCAACATCTATTGCCAATCCATTCACC
GACATGGCCTTGGGCGCTAGAGTCAAGGTTCAGGCGCTCAGTAACGATGCCCCCTCGATGATGATTGCTTGTGGGCTGGC
CATGAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(11-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I1XEV5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

49.716

100

0.497
  comM Acinetobacter nosocomialis M2

49.148

100

0.491
  comM Acinetobacter baylyi ADP1

47.159

100

0.472
  pilM Legionella pneumophila strain ERS1305867

44.253

98.864

0.438

Multiple sequence alignment