Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q7C_RS10675 Genome accession   NC_017856
Coordinates   2218357..2220075 (-) Length   572 a.a.
NCBI ID   WP_014704783.1    Uniprot ID   I1YKC1
Organism   Methylophaga frappieri     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2213357..2225075
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7C_RS10645 (Q7C_2221) - 2214072..2214257 (+) 186 WP_014704776.1 zinc-finger domain-containing protein -
  Q7C_RS10650 (Q7C_2222) mutT 2214254..2214694 (+) 441 WP_014704777.1 8-oxo-dGTP diphosphatase MutT -
  Q7C_RS13550 (Q7C_2223) yacG 2214651..2214845 (-) 195 WP_014704778.1 DNA gyrase inhibitor YacG -
  Q7C_RS10655 (Q7C_2224) zapD 2214845..2215621 (-) 777 WP_014704779.1 cell division protein ZapD -
  Q7C_RS10660 (Q7C_2225) coaE 2215628..2216239 (-) 612 WP_014704780.1 dephospho-CoA kinase -
  Q7C_RS10665 (Q7C_2226) pilD 2216243..2217112 (-) 870 WP_014704781.1 prepilin peptidase Machinery gene
  Q7C_RS10670 (Q7C_2227) pilC 2217112..2218353 (-) 1242 WP_014704782.1 type II secretion system F family protein Machinery gene
  Q7C_RS10675 (Q7C_2228) pilB 2218357..2220075 (-) 1719 WP_014704783.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q7C_RS13555 (Q7C_2229) lptM 2220218..2220346 (+) 129 WP_014704784.1 LPS translocon maturation chaperone LptM -
  Q7C_RS10680 (Q7C_2230) lysA 2220353..2221600 (+) 1248 WP_014704785.1 diaminopimelate decarboxylase -
  Q7C_RS10685 (Q7C_2231) dapF 2221604..2222452 (+) 849 WP_014704786.1 diaminopimelate epimerase -
  Q7C_RS10690 (Q7C_2232) - 2222428..2223132 (+) 705 WP_014704787.1 DUF484 family protein -
  Q7C_RS10695 (Q7C_2233) xerC 2223116..2224036 (+) 921 WP_014704788.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 63135.15 Da        Isoelectric Point: 4.9915

>NTDB_id=51041 Q7C_RS10675 WP_014704783.1 2218357..2220075(-) (pilB) [Methylophaga frappieri]
MANTSVPIRLSGLARRLVNEGLLSEEEAQSAQMAAKQAKQSLVTYLVNHKLLSSQKIADIASQEFGVPLFDIDTMNLELA
PTSLFQEKLIRQHNALPLFQRGKRLFVAVSDPTNLQALDEFQFNTALNTYPVLVDEQKLALSIEKALDKREQQLDEFANA
DLDDIDLGPDDEVDRSADITESNIDDTPVVRFINGIMTQSIKAGASDIHFEPYENKYRVRIRIDGILHETKTAPVALSGR
IAARLKVLSRMNIAERRVPQDGRMRLKLSKNKAIDFRVNTIPTLFGEKIVLRILDPTSAQMGIDALGYEEDQKQIFLDTM
HKPYGMILVTGPTGSGKTVSLYTALNILNTDDRNISTAEDPAEINLPGINQVNVHPQIGLDFSQCLRAFLRQDPDVIMVG
EIRDLETAEIAIKAAQTGHMVFSTLHTNDAPQTLTRLANMGVPAFNIASAVSLIIAQRLARRLCPKCKQPEDVPAETLLA
EGFSQQQVDTGFKPYKAVGCDSCTAGYKGRVGIYQVMPVSEEMGRIIMENGNAIQLADQAEQEGIDDLRASGLKKVAAGL
TSLEEINRVIKE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=51041 Q7C_RS10675 WP_014704783.1 2218357..2220075(-) (pilB) [Methylophaga frappieri]
ATGGCAAATACATCCGTACCGATTCGATTAAGCGGTCTGGCCCGGCGGCTGGTAAATGAAGGCCTGCTTTCGGAAGAAGA
AGCCCAATCTGCGCAAATGGCGGCCAAACAAGCGAAGCAATCGCTGGTTACCTATTTGGTTAATCACAAACTGTTATCCA
GCCAAAAAATCGCGGATATTGCCTCTCAAGAATTCGGTGTTCCTCTATTTGATATCGATACCATGAATCTGGAACTGGCG
CCGACCAGTTTGTTTCAGGAAAAATTGATTCGCCAGCATAACGCGCTGCCTTTATTTCAGCGGGGTAAACGATTATTTGT
CGCCGTTTCAGATCCCACTAATCTGCAGGCGCTGGATGAATTTCAATTTAACACGGCATTAAATACTTATCCGGTTTTGG
TTGATGAACAAAAACTGGCACTAAGTATTGAAAAAGCCTTAGACAAACGCGAACAACAACTAGACGAATTTGCCAATGCA
GACCTTGATGATATTGATCTTGGTCCCGATGACGAGGTTGATCGTTCTGCTGACATTACCGAAAGCAATATTGATGACAC
CCCCGTTGTTCGGTTCATCAATGGCATCATGACCCAGTCTATCAAAGCAGGCGCTTCGGACATTCACTTCGAGCCATATG
AAAATAAATACCGGGTTCGTATTCGTATTGATGGTATTTTGCATGAGACCAAGACCGCACCGGTTGCTTTAAGTGGCCGT
ATTGCAGCACGTTTAAAAGTGCTATCGCGAATGAATATTGCCGAAAGACGAGTCCCTCAAGATGGTCGCATGCGGCTGAA
ATTATCCAAAAACAAAGCCATCGACTTCCGGGTCAACACCATCCCAACGCTGTTTGGTGAAAAAATTGTATTACGGATCC
TGGATCCAACCAGTGCACAGATGGGTATTGATGCGCTCGGTTATGAGGAAGACCAGAAACAAATCTTTCTCGATACCATG
CATAAACCTTATGGCATGATTCTGGTTACTGGCCCGACGGGTAGCGGTAAAACCGTCTCGCTCTATACGGCTCTGAATAT
TCTCAATACCGATGATCGGAATATTTCAACCGCTGAAGACCCCGCAGAAATCAACTTGCCCGGCATTAACCAGGTGAATG
TACATCCGCAAATCGGCCTCGACTTTTCACAATGTTTGCGGGCTTTTTTGCGTCAGGATCCCGATGTCATCATGGTCGGT
GAGATCCGGGATCTGGAAACCGCTGAAATTGCGATTAAAGCGGCACAAACTGGTCATATGGTGTTCTCTACTCTGCATAC
TAATGATGCGCCACAAACCCTGACACGACTTGCCAATATGGGTGTGCCCGCCTTTAATATCGCCTCTGCCGTATCGCTCA
TCATTGCACAGCGGCTGGCTCGCCGATTGTGTCCCAAGTGCAAGCAGCCCGAGGATGTGCCTGCGGAAACTTTGCTAGCA
GAAGGGTTCAGCCAACAACAAGTCGATACCGGTTTTAAACCGTACAAAGCCGTCGGGTGTGATTCCTGTACTGCCGGTTA
CAAAGGCCGCGTGGGGATTTATCAAGTAATGCCTGTTTCTGAAGAAATGGGTCGCATCATTATGGAAAATGGTAATGCCA
TCCAACTGGCCGATCAGGCTGAACAAGAAGGTATTGATGATTTACGGGCATCTGGACTGAAAAAAGTGGCGGCAGGCCTG
ACCAGTCTGGAAGAAATTAATCGCGTGATTAAGGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I1YKC1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.36

98.951

0.558
  pilB Acinetobacter baylyi ADP1

54.766

100

0.552
  pilB Legionella pneumophila strain ERS1305867

52.88

100

0.53
  pilB Vibrio parahaemolyticus RIMD 2210633

49.204

98.776

0.486
  pilB Vibrio cholerae strain A1552

49.909

96.329

0.481
  pilB Vibrio campbellii strain DS40M4

48.233

98.951

0.477
  pilF Neisseria gonorrhoeae MS11

47.943

97.727

0.469
  pilB/pilB1 Synechocystis sp. PCC 6803

37.338

100

0.402
  pilF Thermus thermophilus HB27

37.102

98.951

0.367

Multiple sequence alignment