Detailed information NTG0051039

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: Q7C_RS10665
• Genome accession: NC_017856
• Coordinates: 2216243..2217112 (-)
• Length: 289 a.a.
• NCBI ID: WP_014704781.1
• Uniprot ID: I1YKB9
• Organism: Methylophaga frappieri
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2211243..2222112

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q7C_RS10640 (Q7C_2220)	nadA	2212945..2214057 (+)	1113	WP_014704775.1	quinolinate synthase NadA	-
Q7C_RS10645 (Q7C_2221)	-	2214072..2214257 (+)	186	WP_014704776.1	zinc-finger domain-containing protein	-
Q7C_RS10650 (Q7C_2222)	mutT	2214254..2214694 (+)	441	WP_014704777.1	8-oxo-dGTP diphosphatase MutT	-
Q7C_RS13550 (Q7C_2223)	yacG	2214651..2214845 (-)	195	WP_014704778.1	DNA gyrase inhibitor YacG	-
Q7C_RS10655 (Q7C_2224)	zapD	2214845..2215621 (-)	777	WP_014704779.1	cell division protein ZapD	-
Q7C_RS10660 (Q7C_2225)	coaE	2215628..2216239 (-)	612	WP_014704780.1	dephospho-CoA kinase	-
Q7C_RS10665 (Q7C_2226)	pilD	2216243..2217112 (-)	870	WP_014704781.1	prepilin peptidase	Machinery gene
Q7C_RS10670 (Q7C_2227)	pilC	2217112..2218353 (-)	1242	WP_014704782.1	type II secretion system F family protein	Machinery gene
Q7C_RS10675 (Q7C_2228)	pilB	2218357..2220075 (-)	1719	WP_014704783.1	type IV-A pilus assembly ATPase PilB	Machinery gene
Q7C_RS13555 (Q7C_2229)	lptM	2220218..2220346 (+)	129	WP_014704784.1	LPS translocon maturation chaperone LptM	-
Q7C_RS10680 (Q7C_2230)	lysA	2220353..2221600 (+)	1248	WP_014704785.1	diaminopimelate decarboxylase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 31748.01 Da
• Isoelectric Point: 8.1644

>NTDB_id=51039 Q7C_RS10665 WP_014704781.1 2216243..2217112(-) (pilD) [Methylophaga frappieri]
MPVVDLLVSSTLAFVITATILGLLIGSFLNVVIHRIPLIMQRDWERQCHELMDSKAPTQSTLTLSRPRSRCPDCGKAIPA
LDNIPILSYLILGGRCRACRAPISIRYPLIELLTGVLSAIVAAHFGFSWACLGALLLTWLLIALTFIDVDKQLLPDNLTL
PLLWMGLVFNLFNVFTDLTSAVIGAISGYMALWLVYQLFRLITGKEGMGYGDFKLLAALGAWLGWQFLPVIIILSSLVGA
VLGICLILFKQHQHSQPIPFGPYLAAAGWLALIWGEQLNHAYLRLAGLA

Nucleotide

• Length: 870 bp

>NTDB_id=51039 Q7C_RS10665 WP_014704781.1 2216243..2217112(-) (pilD) [Methylophaga frappieri]
ATGCCGGTTGTCGATTTGCTAGTCAGTTCAACGCTGGCTTTTGTAATAACAGCCACTATTTTAGGATTGCTGATTGGCAG
CTTTTTAAATGTCGTCATCCACCGCATCCCCCTTATCATGCAACGCGACTGGGAGCGACAATGTCATGAGCTAATGGATT
CCAAGGCCCCGACACAATCGACACTGACACTGAGCCGCCCTCGTTCTCGTTGTCCTGATTGTGGCAAGGCTATTCCCGCC
TTGGATAATATTCCTATCCTGAGTTACCTGATCCTTGGTGGGCGCTGCCGGGCATGTCGGGCGCCGATTTCGATTCGCTA
TCCACTGATCGAATTATTAACTGGTGTTTTGTCGGCGATTGTAGCGGCTCACTTTGGGTTTAGCTGGGCATGCCTTGGCG
CTTTATTACTCACCTGGCTATTGATTGCGCTGACCTTTATTGATGTTGATAAACAGCTGTTACCTGACAACTTAACCCTG
CCGCTACTCTGGATGGGGTTGGTATTCAACCTATTTAATGTTTTTACGGATCTGACCTCGGCAGTGATAGGCGCTATCTC
TGGCTATATGGCGCTATGGCTGGTTTATCAACTATTTCGACTGATTACGGGCAAAGAAGGCATGGGCTACGGTGATTTCA
AATTACTGGCGGCTTTAGGCGCTTGGCTGGGCTGGCAGTTTTTACCCGTCATTATTATTTTGTCGTCATTAGTGGGGGCA
GTGCTTGGAATTTGCCTTATCCTGTTTAAACAGCATCAACATAGTCAGCCGATCCCGTTTGGCCCCTATCTGGCTGCAGC
TGGCTGGCTGGCCTTGATATGGGGCGAACAACTTAATCATGCCTATTTACGGCTGGCAGGACTGGCTTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

• Type IV leader peptidase family (Peptidase_A24) (136-244)

3D structure

• AlphaFold DB: I1YKB9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	54.579	94.464	0.516
pilD	Vibrio cholerae strain A1552	53.48	94.464	0.505
pilD	Acinetobacter nosocomialis M2	51.59	97.924	0.505
pilD	Acinetobacter baumannii D1279779	51.237	97.924	0.502
pilD	Neisseria gonorrhoeae MS11	52.768	93.772	0.495