Detailed information NTG0509134

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: I5Z97_RS07535
• Genome accession: NZ_CP065386
• Coordinates: 1572990..1574375 (+)
• Length: 461 a.a.
• NCBI ID: WP_196980196.1
• Uniprot ID: -
• Organism: Glaesserella parasuis strain LHDR_HPS_1_2
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1567990..1579375

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I5Z97_RS07515 (I5Z97_07505)	gpt	1569593..1570072 (+)	480	WP_160403664.1	xanthine phosphoribosyltransferase	-
I5Z97_RS07520 (I5Z97_07510)	gmk	1570235..1570861 (+)	627	WP_196980194.1	guanylate kinase	-
I5Z97_RS07525 (I5Z97_07515)	radA	1570914..1572290 (-)	1377	WP_005711784.1	DNA repair protein RadA	-
I5Z97_RS07530 (I5Z97_07520)	pilA	1572478..1572942 (+)	465	WP_196980195.1	pilin	Machinery gene
I5Z97_RS07535 (I5Z97_07525)	pilB	1572990..1574375 (+)	1386	WP_196980196.1	GspE/PulE family protein	Machinery gene
I5Z97_RS07540 (I5Z97_07530)	pilC	1574368..1575564 (+)	1197	WP_196980197.1	type II secretion system F family protein	Machinery gene
I5Z97_RS07545 (I5Z97_07535)	-	1575561..1576229 (+)	669	WP_196980198.1	prepilin peptidase	-
I5Z97_RS07550 (I5Z97_07540)	dapE	1576313..1577446 (+)	1134	WP_196980199.1	succinyl-diaminopimelate desuccinylase	-
I5Z97_RS07555 (I5Z97_07545)	clpX	1577520..1578770 (-)	1251	WP_196980200.1	ATP-dependent protease ATP-binding subunit ClpX	-
I5Z97_RS07560 (I5Z97_07550)	clpP	1578777..1579358 (-)	582	WP_005711766.1	ATP-dependent Clp endopeptidase proteolytic subunit ClpP	-

Sequence

Protein

• Length: 461 a.a.
• Molecular weight: 52618.28 Da
• Isoelectric Point: 4.9724

>NTDB_id=509134 I5Z97_RS07535 WP_196980196.1 1572990..1574375(+) (pilB) [Glaesserella parasuis strain LHDR_HPS_1_2]
MSQYTVCEQQTERIFDISPSRWQQNCEEKELLLRYLAVPVQETEHKLWLAVDDENNLTACEIFAFMTHKQIEPVVIASDE
LKYLLNALSPEQQPFYEESELAFAEQEQEQLNLSDPIIQLLDNLFKFCLAQNASDIHIEPRKQKLIIRLRIDGVLHLYKS
LSIQLASRLTSRIKLLAKLDISELRQPQDGQFSFTTALADTLDFRVSSLPTIYGEKLVLRLQKNKPTSFDFLQLGFNPQQ
QTTLLNALKQPQGLILVTGPTGSGKSITLYSALNYLNQMDKHILTAEDPVEIEIDGLIQTQVNQSINLDFSQLLRTFLRQ
DPDIIMLGEIRDEESAKMALRASQTGHLVLSTLHTNDALSAVERLLQLGIQEYEIQNSLLLVIAQRLVRKLCKKCCGTGC
DQCYQGYKGRIGIYQCLSRTAKKFDKQTAYLDFSSLRESAKQKIEQQLTNEIEVDRVLGDE

Nucleotide

• Length: 1386 bp

>NTDB_id=509134 I5Z97_RS07535 WP_196980196.1 1572990..1574375(+) (pilB) [Glaesserella parasuis strain LHDR_HPS_1_2]
ATGTCCCAATACACCGTCTGCGAACAACAAACCGAACGCATTTTTGATATATCGCCTAGCCGTTGGCAACAAAATTGCGA
AGAAAAAGAGCTGTTATTGCGTTATCTTGCCGTTCCTGTGCAAGAAACTGAGCATAAATTATGGCTTGCCGTTGATGATG
AAAACAATCTCACCGCTTGCGAAATCTTTGCTTTTATGACCCATAAGCAGATTGAGCCTGTAGTTATTGCTTCTGATGAA
CTTAAATACTTGCTTAACGCACTCTCGCCTGAGCAACAGCCCTTTTATGAAGAGAGCGAACTGGCTTTCGCAGAACAAGA
GCAAGAACAGCTCAACTTAAGCGATCCGATTATTCAACTGCTCGATAATCTTTTTAAATTCTGTTTAGCCCAAAATGCGT
CGGACATTCATATTGAACCACGCAAGCAAAAATTGATTATTCGCCTACGCATTGACGGTGTTTTGCACCTGTATAAAAGC
CTGTCTATTCAACTGGCAAGCCGACTTACTTCACGGATAAAACTGCTGGCGAAGCTAGATATTAGTGAATTACGCCAGCC
ACAAGATGGGCAATTTAGCTTTACCACCGCCTTGGCGGACACCCTTGATTTTCGGGTATCTAGCCTGCCGACGATCTACG
GCGAAAAACTGGTATTGCGCCTACAAAAAAACAAACCAACCAGTTTTGATTTTCTGCAACTTGGCTTTAATCCACAACAG
CAGACCACACTACTCAACGCCTTGAAACAACCGCAAGGGCTGATTTTAGTGACAGGACCAACGGGCAGTGGCAAAAGTAT
CACCCTTTACAGCGCCTTAAACTATCTCAACCAAATGGATAAACATATTCTCACCGCCGAAGATCCCGTGGAAATTGAGA
TTGACGGCTTAATTCAAACCCAAGTCAATCAAAGTATTAACCTTGATTTTAGCCAGCTGTTACGCACCTTTCTACGCCAA
GATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAGAGTGCAAAAATGGCATTAAGAGCAAGCCAGACGGGGCA
TTTAGTCCTTTCAACCCTGCATACCAATGACGCACTATCAGCGGTCGAACGTTTGTTACAACTAGGTATTCAAGAGTATG
AAATCCAAAATTCGCTATTGTTAGTGATTGCACAACGCCTTGTGCGTAAGCTCTGCAAAAAGTGTTGTGGCACAGGCTGT
GACCAATGCTATCAAGGCTATAAAGGGCGGATCGGGATTTACCAATGCTTAAGCAGAACTGCAAAAAAATTCGATAAACA
GACCGCTTACTTGGACTTTTCCAGCTTACGAGAGAGTGCCAAACAGAAAATTGAACAACAACTAACCAACGAAATCGAAG
TGGATAGGGTATTAGGCGATGAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (129-390)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (24-92)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Glaesserella parasuis strain SC1401	96.529	100	0.965
pilB	Haemophilus influenzae 86-028NP	56.957	99.783	0.568
pilB	Haemophilus influenzae Rd KW20	57.582	98.698	0.568
pilB	Vibrio cholerae strain A1552	38.554	100	0.416
pilB	Vibrio parahaemolyticus RIMD 2210633	39.004	100	0.408
pilB	Vibrio campbellii strain DS40M4	39.085	100	0.408
pilB	Acinetobacter baylyi ADP1	36.832	100	0.403
pilB	Acinetobacter baumannii D1279779	35.992	100	0.401
pilB	Legionella pneumophila strain ERS1305867	40.909	95.445	0.39
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.085	100	0.388
pilF	Neisseria gonorrhoeae MS11	36.421	100	0.375