Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   IT970_RS13175 Genome accession   NZ_CP065231
Coordinates   2919805..2921031 (+) Length   408 a.a.
NCBI ID   WP_118844622.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. A41-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2914805..2926031
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IT970_RS13145 (IT970_13140) ampE 2915231..2916082 (-) 852 WP_196857406.1 beta-lactamase regulator AmpE -
  IT970_RS13150 (IT970_13145) ampD 2916106..2916648 (-) 543 WP_118844619.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IT970_RS13155 (IT970_13150) nadC 2916838..2917686 (+) 849 WP_074988951.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IT970_RS13160 (IT970_13155) - 2918184..2918624 (+) 441 WP_118844620.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  IT970_RS16105 (IT970_13160) - 2918643..2919068 (+) 426 WP_118844621.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  IT970_RS13170 (IT970_13165) - 2919065..2919673 (-) 609 WP_118844822.1 fimbrial assembly protein -
  IT970_RS13175 (IT970_13170) pilC 2919805..2921031 (+) 1227 WP_118844622.1 type II secretion system F family protein Machinery gene
  IT970_RS13180 (IT970_13175) pilD 2921211..2922107 (+) 897 WP_118844623.1 A24 family peptidase Machinery gene
  IT970_RS13185 (IT970_13180) coaE 2922110..2922706 (+) 597 WP_118844624.1 dephospho-CoA kinase -
  IT970_RS13190 (IT970_13185) pilB 2922881..2924557 (+) 1677 WP_118844625.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IT970_RS13195 (IT970_13190) yacG 2924625..2924858 (+) 234 WP_036965896.1 DNA gyrase inhibitor YacG -
  IT970_RS13200 (IT970_13195) - 2924920..2925450 (-) 531 WP_118844626.1 Spy/CpxP family protein refolding chaperone -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44443.60 Da        Isoelectric Point: 9.6292

>NTDB_id=508274 IT970_RS13175 WP_118844622.1 2919805..2921031(+) (pilC) [Pseudoalteromonas sp. A41-2]
MSKVQEKQSLDTFEWVGVSARGKKLEGELSGQSIALVKAQLRKQGITPSKVKRKAKPLFGTRVQKITPKDIALITRQIAT
MLMAGVPLIQSIEMIGSGAKNKSVARLMEEIGDEVKAGVPLSQALRKHPRYFDDLYCDLVASGEQSGALDKIFDRVALYK
EKAEALKSKIKKAMFYPIAVLVVALIVTSILLIFVVPQFQDIFNGFGAELPAFTLMVIGISEFMQEYWWIMLIAVIAFGY
AYKEALLKSKKLRDANDRAILKLPVIGMILNKAAVARYARTLSTTFAAGVPLVDALDSAAGASGNAVYRYAILEIKAEVS
SGNLMNWAMRNSKIFPDMVIQMVAIGEESGSLDGMLAKVATIYEQEVDDAVDGLSSLLEPLIMAVLGVLVGGLIIAMYLP
IFQLGSVI

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=508274 IT970_RS13175 WP_118844622.1 2919805..2921031(+) (pilC) [Pseudoalteromonas sp. A41-2]
ATGAGTAAAGTTCAAGAAAAGCAATCTTTAGATACTTTTGAATGGGTTGGTGTAAGTGCTCGAGGCAAGAAATTAGAAGG
AGAGCTTTCAGGGCAAAGCATTGCCTTAGTCAAAGCTCAATTGCGTAAGCAAGGCATCACCCCTTCAAAAGTAAAACGTA
AGGCAAAACCTTTATTTGGAACTCGGGTTCAAAAAATTACCCCTAAAGACATTGCTTTAATTACACGCCAAATAGCGACC
ATGCTAATGGCAGGTGTACCGCTTATTCAATCTATTGAAATGATAGGTTCAGGTGCTAAGAATAAAAGTGTTGCTAGGCT
GATGGAAGAAATTGGTGACGAAGTGAAAGCGGGTGTGCCACTCTCGCAAGCTTTACGCAAACACCCCCGCTATTTTGATG
ATTTATATTGCGACCTTGTTGCGTCTGGTGAGCAATCGGGTGCCCTTGATAAAATTTTCGACCGTGTCGCACTTTATAAA
GAAAAAGCCGAAGCATTAAAGTCTAAAATCAAAAAAGCAATGTTCTACCCGATTGCGGTATTAGTTGTGGCCTTAATCGT
TACCTCAATCTTACTTATTTTTGTGGTGCCTCAATTCCAAGATATTTTTAACGGCTTTGGTGCTGAACTTCCTGCCTTTA
CTTTAATGGTGATAGGTATATCTGAATTTATGCAAGAGTATTGGTGGATAATGCTGATTGCTGTTATTGCTTTTGGCTAT
GCCTATAAAGAAGCCCTGCTTAAAAGCAAAAAACTCAGAGATGCCAACGACAGAGCTATATTAAAGTTACCTGTTATTGG
CATGATTTTAAATAAAGCAGCAGTAGCTCGCTATGCACGCACTCTTTCAACAACCTTTGCTGCGGGTGTACCCTTGGTTG
ATGCGCTCGATTCAGCCGCGGGAGCCTCAGGTAATGCGGTGTACCGTTACGCTATTTTAGAAATTAAAGCAGAAGTTAGC
TCAGGTAATTTGATGAACTGGGCGATGCGGAACTCTAAAATATTTCCTGATATGGTGATCCAAATGGTTGCAATTGGTGA
AGAGTCAGGCTCTTTAGATGGCATGCTTGCCAAAGTAGCCACTATTTATGAGCAAGAGGTTGATGACGCAGTCGATGGCC
TTTCAAGCTTATTAGAGCCGCTGATCATGGCTGTACTTGGTGTGCTGGTCGGTGGTTTAATTATTGCCATGTATTTACCT
ATTTTCCAGTTAGGTTCCGTCATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(279-400)

Type II secretion system (T2SS), protein F (T2SSF)

(75-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

63.384

97.059

0.615
  pilC Acinetobacter baumannii D1279779

55.911

99.51

0.556
  pilC Acinetobacter baylyi ADP1

54.568

99.265

0.542
  pilC Legionella pneumophila strain ERS1305867

55.164

97.304

0.537
  pilC Vibrio cholerae strain A1552

44.254

100

0.444
  pilG Neisseria meningitidis 44/76-A

43.766

96.324

0.422
  pilG Neisseria gonorrhoeae MS11

43.511

96.324

0.419
  pilC Vibrio campbellii strain DS40M4

42.607

97.794

0.417