Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   I5R27_RS02755 Genome accession   NZ_CP065202
Coordinates   575425..576756 (+) Length   443 a.a.
NCBI ID   WP_196883630.1    Uniprot ID   A0A9Q6VLY9
Organism   Pseudomonas fragi strain A13BB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 570425..581756
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5R27_RS02735 (I5R27_02735) pilM 572777..573703 (+) 927 WP_196883626.1 type IV pilus assembly protein PilM -
  I5R27_RS02740 (I5R27_02740) - 573703..574269 (+) 567 WP_016783186.1 PilN domain-containing protein -
  I5R27_RS02745 (I5R27_02745) - 574269..574889 (+) 621 WP_196883628.1 type 4a pilus biogenesis protein PilO -
  I5R27_RS02750 (I5R27_02750) - 574886..575413 (+) 528 WP_169846156.1 pilus assembly protein PilP -
  I5R27_RS02755 (I5R27_02755) pilQ 575425..576756 (+) 1332 WP_196883630.1 type IV pilus secretin PilQ Machinery gene
  I5R27_RS02760 (I5R27_02760) aroK 576761..577279 (+) 519 WP_029611693.1 shikimate kinase AroK -
  I5R27_RS02765 (I5R27_02765) aroB 577436..578536 (+) 1101 WP_016783191.1 3-dehydroquinate synthase -
  I5R27_RS02770 (I5R27_02770) - 578547..580124 (+) 1578 WP_196883632.1 AAA family ATPase -

Sequence

Protein


Download         Length: 443 a.a.        Molecular weight: 47584.57 Da        Isoelectric Point: 5.0583

>NTDB_id=507949 I5R27_RS02755 WP_196883630.1 575425..576756(+) (pilQ) [Pseudomonas fragi strain A13BB]
MKRISPVCVLALWSALNSQETLAAGQAAPSSIASAQYVGEPMSLNVQNLEVRTVLQSLADFNGFNLMVSDTVKGSITLSL
QDVPWDQALALVLGSKGLGKRLEGNVLWVAPLDEIAARERPEPAPLRRELVQVNYARAADIVMLFQSVTGGQAGLEGQGT
ITVDERTNNIIAFESSERLDELRRIVAQLDVPVRQVMIEAWIVEAGVDFEKSLGVRWGGASSNSGLWSASGITQPLQSGA
APGNASAPFVDLGVSRSSSGLGIAFVTNNVLLDLELTAMEKTGNGEIISQPKVVTADKETAKILKGTQIPYQQASKTGAT
SVSFKEASLSLEVTPQITPDNSVIMAVVVTKDEPDYMSRLQDVPPIKKNEVRAKVLVKDGETIVIGGVFSKTQSKVVDKV
PFLGDLPYVGRLFHRDVVLEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 1332 bp        

>NTDB_id=507949 I5R27_RS02755 WP_196883630.1 575425..576756(+) (pilQ) [Pseudomonas fragi strain A13BB]
ATGAAAAGGATTTCACCGGTCTGTGTACTGGCGCTATGGAGTGCGCTGAATTCGCAGGAAACACTGGCGGCCGGGCAGGC
CGCGCCTTCATCCATCGCGAGCGCGCAGTATGTGGGCGAACCCATGTCCCTGAACGTGCAGAACCTTGAAGTACGCACGG
TGCTGCAATCGCTGGCGGATTTTAACGGGTTCAACCTGATGGTCAGCGACACCGTGAAAGGCAGTATCACCCTCAGTTTG
CAGGACGTGCCTTGGGATCAGGCGCTGGCTCTGGTGCTTGGCAGCAAGGGCCTGGGCAAGCGCCTTGAGGGTAATGTGCT
GTGGGTCGCGCCCCTGGACGAGATCGCCGCCCGCGAGCGGCCAGAGCCTGCGCCTTTGCGTCGGGAGCTGGTGCAGGTCA
ATTACGCCAGGGCCGCAGATATCGTCATGCTGTTCCAGTCAGTGACCGGCGGTCAGGCCGGTCTCGAGGGGCAGGGCACG
ATCACGGTAGATGAGCGCACCAATAACATCATTGCCTTTGAATCCAGTGAACGACTGGATGAGCTGCGGCGCATCGTGGC
CCAACTGGACGTGCCGGTGCGCCAGGTGATGATTGAGGCCTGGATTGTCGAGGCGGGAGTGGACTTCGAAAAAAGCCTGG
GTGTTCGCTGGGGTGGGGCGTCAAGCAACAGCGGCCTGTGGAGTGCCAGCGGTATCACCCAGCCACTGCAGTCAGGGGCT
GCGCCCGGCAACGCCAGCGCGCCTTTTGTCGACCTGGGGGTTTCGCGCAGCTCATCGGGGTTGGGCATCGCCTTTGTGAC
CAACAATGTGCTGCTGGACCTGGAGCTGACGGCCATGGAAAAAACCGGTAATGGCGAAATCATCTCGCAGCCCAAGGTGG
TTACCGCAGACAAGGAAACCGCAAAAATCCTCAAAGGCACGCAAATTCCCTATCAACAGGCCAGCAAAACCGGCGCCACA
TCGGTCTCGTTCAAGGAGGCCTCGCTGTCGCTGGAAGTCACGCCGCAAATCACCCCGGACAACAGTGTCATCATGGCCGT
CGTGGTGACCAAGGATGAGCCTGACTACATGAGCCGGTTGCAGGATGTGCCGCCCATCAAGAAGAACGAGGTCAGGGCCA
AGGTGTTGGTCAAGGATGGCGAAACCATCGTGATTGGGGGGGTGTTCTCAAAAACTCAAAGCAAAGTGGTAGATAAAGTG
CCATTTTTGGGCGATCTGCCGTATGTTGGCCGACTTTTCCATAGAGACGTTGTCCTGGAAAAAAAATCCGAACTGCTGGT
GTTCCTGACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(278-434)

Bacterial type II/III secretion system short domain (Secretin_N)

(130-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

64.76

98.646

0.639
  comQ Acinetobacter baylyi ADP1

42.411

100

0.429
  pilQ Acinetobacter baumannii strain A118

39.362

100

0.418
  pilQ Acinetobacter baumannii D1279779

39.362

100

0.418
  pilQ Vibrio campbellii strain DS40M4

40.222

100

0.409
  pilQ Legionella pneumophila strain Lp02

40.826

98.42

0.402
  pilQ Legionella pneumophila strain ERS1305867

40.596

98.42

0.4
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.72

96.614

0.384
  pilQ Vibrio cholerae strain A1552

39.72

96.614

0.384