Detailed information NTG0504711

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IWH25_RS15595
• Genome accession: NZ_CP064781
• Coordinates: 3263954..3265147 (+)
• Length: 397 a.a.
• NCBI ID: WP_203386683.1
• Uniprot ID: A0A974PY63
• Organism: Azospira restricta strain DSM 18626
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3258954..3270147

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IWH25_RS15580 (IWH25_15570)	-	3259448..3261754 (-)	2307	WP_203386680.1	DNA translocase FtsK	-
IWH25_RS15585 (IWH25_15575)	trxB	3261950..3262912 (+)	963	WP_203386681.1	thioredoxin-disulfide reductase	-
IWH25_RS15590 (IWH25_15580)	-	3262928..3263932 (+)	1005	WP_203386682.1	Smr/MutS family protein	-
IWH25_RS15595 (IWH25_15585)	pilU	3263954..3265147 (+)	1194	WP_203386683.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IWH25_RS15600 (IWH25_15590)	-	3265294..3269334 (-)	4041	WP_203386684.1	helix-turn-helix transcriptional regulator	-
IWH25_RS15605 (IWH25_15595)	glnK	3269591..3269929 (-)	339	WP_203386685.1	P-II family nitrogen regulator	-

Sequence

Protein

• Length: 397 a.a.
• Molecular weight: 44100.75 Da
• Isoelectric Point: 6.7804

>NTDB_id=504711 IWH25_RS15595 WP_203386683.1 3263954..3265147(+) (pilU) [Azospira restricta strain DSM 18626]
MIQNEDIIGDELKNYIHALLKAMVQHGGSDLFISKDFPPTMKIHGTMKALSSQKLSADVSKKLAWILMNRDQRTEFEREM
ECNFAISLPGIARFRVNVFVQQQAVGMVIRTITSEIPNFEKLGLPPVLKDVIMAKRGLVLVVGGTGSGKSTTMAAMIDHR
NSTAPGHIITVEDPVEYVHTSKQCLITHREVGVDTMSWHAALKNTLRQAPDVILIGEIRDLETMEHALAFAETGHLCMGT
LHANNANQTLDRIVNFFPDERKPQVLMDIASNLKAVVSQRLVRSQDGKRKAAIEILLNTATVAELLSKGQSAAIKPIMDK
SRQLGMQTFDQALFDLYDQGHISYDEAIRNADSTNELRLAIKLKSKRGEPSTASGMSLSIEEEKKEEKAEGEEEEKK

Nucleotide

• Length: 1194 bp

>NTDB_id=504711 IWH25_RS15595 WP_203386683.1 3263954..3265147(+) (pilU) [Azospira restricta strain DSM 18626]
ATGATCCAGAACGAAGACATCATCGGCGACGAGCTGAAGAACTACATCCATGCGCTGTTGAAGGCGATGGTGCAGCACGG
CGGCTCCGACCTGTTCATCTCGAAGGACTTCCCGCCGACGATGAAGATCCACGGGACGATGAAGGCGCTGTCGTCGCAGA
AGCTCTCCGCCGACGTCTCGAAGAAGCTGGCGTGGATCCTGATGAACCGCGACCAGCGGACCGAATTCGAGCGCGAGATG
GAATGCAACTTCGCGATCTCGCTGCCCGGCATCGCCCGCTTCCGCGTCAACGTCTTCGTCCAGCAGCAGGCCGTGGGCAT
GGTCATCCGCACCATCACCAGCGAAATCCCGAACTTCGAGAAACTGGGCCTGCCGCCGGTGCTGAAGGACGTGATCATGG
CCAAGCGCGGCCTCGTGCTGGTCGTCGGCGGCACCGGCTCCGGCAAGTCGACGACGATGGCGGCGATGATCGACCACCGC
AACAGCACGGCGCCCGGCCACATCATCACCGTCGAGGACCCGGTCGAGTACGTGCACACCTCCAAGCAATGCCTGATCAC
GCACCGCGAGGTCGGCGTCGACACGATGTCCTGGCACGCCGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTCATCC
TGATCGGCGAGATCCGCGACCTGGAGACGATGGAGCACGCGCTGGCCTTCGCCGAGACCGGCCACCTGTGCATGGGCACG
CTGCACGCCAACAACGCCAACCAGACGCTGGACCGCATCGTCAATTTCTTCCCCGACGAGCGCAAGCCGCAGGTGCTGAT
GGACATCGCCTCCAACCTCAAGGCGGTCGTCTCGCAACGCCTCGTCCGCTCGCAGGACGGCAAGCGCAAGGCGGCGATCG
AGATCCTGCTGAACACCGCGACGGTCGCCGAACTGCTGTCCAAGGGCCAGTCGGCGGCGATCAAGCCGATCATGGACAAG
AGCCGCCAGCTCGGCATGCAGACCTTCGACCAGGCCCTCTTCGACCTCTACGACCAGGGCCACATCAGCTACGACGAAGC
CATCCGCAACGCCGACTCGACCAACGAGCTGCGGCTGGCGATCAAGCTGAAGAGTAAGCGCGGGGAGCCGTCGACGGCGA
GCGGCATGTCGCTGTCGATCGAGGAGGAAAAGAAGGAAGAGAAGGCCGAGGGCGAAGAGGAAGAGAAGAAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (105-284)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.342	91.94	0.509
pilU	Acinetobacter baylyi ADP1	53.107	89.169	0.474
pilU	Vibrio cholerae strain A1552	50.556	90.68	0.458
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.092	87.406	0.385
pilT	Legionella pneumophila strain ERS1305867	44.817	82.62	0.37
pilT	Legionella pneumophila strain Lp02	44.817	82.62	0.37
pilT	Pseudomonas aeruginosa PAK	43.675	83.627	0.365
pilT	Pseudomonas stutzeri DSM 10701	43.373	83.627	0.363